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Article
Determination of Serum CA125 and evaluate its efficiency as screening tool For Early Detection of Ovarian Tumors
تحديد المستوى المصلي للمستضد السرطاني 125 وتقييم كفاءته كوسيلة فحص للتشخيص المبكر لأورام المبيض

Authors: Munther J. Hussein منذر جعفر حسين --- Maisaa G. Jumaa ميساء غازي جمعة --- Khalid A. Habib خالد عبد الرزاق حبيب
Journal: Baghdad Science Journal مجلة بغداد للعلوم ISSN: 20788665 24117986 Year: 2015 Volume: 12 Issue: 1 Pages: 55-62
Publisher: Baghdad University جامعة بغداد

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Abstract

Epithelial ovarian cancer is the leading cause of cancer deaths in women. To date, an effective screening tool for ovarian cancer has not been identified Several clinical and biological factors including serum cancer antigen 125 (CA- 125) have been assessed for prognostic and predictive relevance CA-125 is an epithelial marker derived from coelomic epithelium. It is elevated in 90% of advanced ovarian cancers and in 50% of early ovarian cancers while 20% of ovarian cancers have low or no expression of CA- 125 CA-125 concentrations were measured by Mini Vidas test (VIDAS CA125 II / BIOMERIEUX / France). The median CA-125 levels were significantly higher in the sera of ovarian cancer patients than in those with benign tumors and in healthy controls. However in correlation with stages the results showed that Patients with stage II have highly significant differences in level of serum CA125 compare with stage I in and stage III.CA125 showed low sensitivity to detect stage I carcinoma of the ovary which limits its value as an initial screening tool therefore combining of CA125 with other markers might enable improved early detection of ovarian cancer as compared with use of this marker alone.

يعتبر سرطان المبيض الطلائي احد المسببات المؤدية الى الموت من بين الامراض النسائية.الى هذا التاريخ لم يتم تشخيص وسيلة فحص فعالة للكشف عن سرطان المبيض. العديد من العوامل السريرية و البايلوجية ومنها الانتجين السرطاني 125 تم تقييمها من حيث علاقتها بالمرض وتقدمه. الانتجين السرطاني 125 هو معلم طلائي مستمد من التجويف الطلائي وهو يرتفع في حوالي 90% من حالات سرطان المبيض المتقدمة وفقط 50% من حالات سرطان المبيض المبكر في حين 20% من هذه الحالات لا تظهر او تظهر تعبير منخفض لهذا الانتيجين السرطاني. تم قياس تراكيز للانتجين السرطاني 125 باستخدام اختبار MiniVidas test. وقد اظهرت النتائج ان متوسط تركيز للانتجين السرطاني 125 قد اظهر فروقات عالية المعنوية في مصول المريضات المصابات بسرطان المبيض عند المقارنة مع تلك الماخوذة من المريضات المصابات بأورام المبيض الحميدة ومجموعة السيطرة.لكن عند المقارنة مع مراحل الورم اظهرت النتائج ان عينات المرضى في المرحلة الثانية قد اظهروا فروقات عالية المعنوية عند المقارنة مع مرضى المرحلتين الاولى والثالثة. ان الانتجين السرطاني 125 يعتبر واطئ الحساسية في تشخيص المرحلة السرطانية الاولى للمبيض مما يحدد قيمته كوسيلة اولية للفحص لذلك فان ارفاق الانتجين السرطاني 125 بمعلمات اخرى قد يساهم في تحسن عملية التشخيص المبكر لسرطان المبيض بالمقارنة مع استخدامه بشكل مفرد.

Keywords

Ovarian cancer --- CA125 --- Serum


Article
EXPRESSION OF P53 PROTEIN IN NEOPLASTIC AND NON NEOPLASTIC OVARIAN LESIONS

Authors: Fahem M. Mahmood فاهم محسن محمود --- Haider S. Kadhim حيدر صباح كاظم --- Liqaa R. Al Khuzaee لقاء رياض موسى الخزاعي
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2013 Volume: 11 Issue: 3 Pages: 280-284
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Ovarian cancer is one of the most common causes of gynecologic neoplasm all over the world.Objective:The objective is to shed light on the role of p53 protein and patient`s age in the pathogenesis of ovarian lesions.Methods:Paraffin embedded blocks of 62 patients with ovarian lesions were studied. Thirty-five cases of surface epithelial ovarian tumors, (31 cases of invasive surface epithelial ovarian tumors, and 4 cases of borderline intermediate malignancy cases of neoplastic ovarian cystic lesions). In addition, eighteen cases of benign neoplastic ovarian cystic lesions and 9 cases of non- neoplastic functional one were enrolled in this study. All of cases included, were stained with p53 by immunohistochemistry.Results:Immunohistochemistry for p53 showed that malignant cases were positive for p53 while all benign cases were negative for p53 and the borderline cases were also negative for p53. The non-neoplastic cases were negative for p53. There is a significant statistical difference in P53 expression in malignant cases compared to other groups (P < 0.001). A significant difference in mean age of malignant and border line cases in comparison with benign and non-neoplastic cases; (P < 0.001).Conclusion:Protein p53 may play a role in the pathogenesis of malignant ovarian cancer but not in benign lesions. The age of the patient has a role as a risk factor in ovarian lesions.Keywords:Ovarian lesion, ovarian cancer, p53, immunohistochemistry.


Article
Performance of Ultrasound as a Second Line Test to Serum Ca125 in Ovarian Cancer Screening in Postmenopausal Women

Authors: Nada Salih Amin --- Asmaa Mohammed Abid
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 1 Pages: 29-36
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND: Epithelial ovarian cancer is uncommon before 40 years of age but the incidence then rises steeply until the mid sixth and seventh decades for which performance of transvaginal ultrasonography as a screening test for ovarian cancer in asymptomatic postmenopausal women with an elevated serum CA 125 had been performed.OBJECTIVE: Prospective ovarian cancer screening trial had been performed to estimate sensitivity, specifity and positive predictive value of different ultrasound criteria for detection of index cancer (e.g. primary invasive epithelial carcinoma of ovary) in postmenopausal women.PATIENTS AND METHODS:This study was carried out at the department of obstetrics and gynecology in AL-Yarmouk Teaching Hospital from October 2002 through October2003.The study included 110 Postmenopausal women ≥ 45 years, they underwent measurement of serum CA125.Women with CA 125 of 30 IU/ml (or more) were recalled for an ultrasound examination. RESULTS: Of the 110 women included in this study, 9 women underwent 30 scans during a follow up of one year. The sensitivity for detection of ovarian cancer of different ultrasound criteria was 100% for abnormal ovarian morphology, 100% for abnormal ovarian volume and 50% for complex abnormal ovarian morphology. The highest specificity (100%) and positive predictive value (100 %) was achieved by using complex abnormal ovarian morphology.CONCLUSION: A variety of ultrasound criteria had achieved high sensitivity, specificity and positive predictive value for ovarian cancer screening in postmenopausal women with an elevated CA 125. Ovarian morphology and ovarian volume used to interpret ultrasound had achieved increased sensitivity for ovarian cancer screening. While complex abnormal ovarian morphology had achieved increased in the specificity and the positive predictive value for ovarian cancer screening.KEYWORDS: ovarian cancer, CA125, transvaginal ultrasound.


Article
Expression of Carbonic Anhydrase IX (CA9) in Human Tumor Tissue of Patients with Ovarian Cancer

Author: Jawdat N. Gaaib, Maisaa G. Jumaa
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 2 Pages: 81-88
Publisher: Baghdad University جامعة بغداد

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Abstract

: The aim of the present study is to determine the expression levels of the CAIX gene in the Paraffin-embedded tissue blocks from 43 patients with different stages of newly diagnosed ovarian cancer as well as 14 tissue samples of patients with benign ovarian tumors were used as a control group. The study performed using a reverse transcription-quantitative polymerase chain reaction (RT-qPCR). According to malignancy status the percentage of patients with CAIX-positive gene expression was significantly higher in compare with benign tumor patients. Depending on the cutoff value, the present study showed that 40(93.02%) of ovarian cancer samples were CAIX positive, which is statistically significant comparing with CAIX-negative samples 3(6.97%). Statistically there was no significant difference in the levels of gene expression with age, menopausal state, and family history. In correlation with histopathologic tumor types and tumor stage, the present study showed significant associations of high level of CAIX gene expression with mucinous histopathologic tumor types( p<0.05), and stage I of ovarian cancer ( p<0. 0.05). The present study concluded that the possibility of using CAIX gene as a useful tool for discriminating malignant ovarian tumors from non-malignant ones. We also demonstrated the diagnostic value of CAIX gene for early diagnosis of ovarian cancer.


Article
Vascular endothelial growth factor gene expression and polymorphism C936T in Iraqi patients with ovarian carcinoma

Author: Maisaa G. Jumaa1 , Khalid K. Tobal2 , Mohammed Ghanim3
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 3 Pages: 137-148
Publisher: Baghdad University جامعة بغداد

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Abstract

The present study aimed to shed light on the association between the angiogenic factor VEGF gene expression and its genetic polymorphism VEGF C936T in the angiogenesis of ovarian cancer. A total of 44 Paraffin-embedded tissue blocks from patients with different stages of newly diagnosed ovarian cancer were provided by certain Iraqi hospitals as well as 14 samples of patients with benign ovarian tumors tissues as a control group were used in this study. The results detected that VEGF mRNA in ovarian cancer samples was statistically significant compared to benign tumors(p value=0.039<0.05). The samples were divided into high and low mRNA-expression depending on the mean value of VEGF gene expression in benign tumors which used as a cutoff, the results showed that high statistical significant differences (P value = 0.0023<0.01) between high mRNA-expressing samples 30(76.9%), and low mRNA-expressing samples 9(23.07%). Statistical no significant differences were detected in compare with other histopathology tumor types. In correlation with stages, statistically significant difference was found between 31 (79.4%) of samples with stage I which showed the highest level of expression(P=0.0210<0.05). The result of VEGF C936T polymorphism showed that out of 42 ovarian cancer patients 24(57.14%) were homozygous for the C/C genotype, 11(26.2%) were heterozygous C/T and 7(16.66%) were homozygous T/T. High significant prevalence of the VEGF 936C allele was detected in both ovarian cancer patients (P value 0.0019 <0.01) and benign ovarian tumors (P value 0.0026 <0.01) as compared with VEGF 936T allele. The study showed that the average of VEGF gene expression in ovarian cancer patients carrying the +936CT+TT genotypes (1.815116, 2.298769) was significantly lower than that in ovarian cancer patients with the VEGF+936CC genotype (4.395884). Same results were obtained from patients with benign ovarian tumors. In conclusion the present study investigated that the presence of the VEGF+936 T allele is associated with a decreased risk of ovarian cancer since the C936T polymorphism has been reported to be associated with lower VEGF plasma levels.


Article
Clinical and Histopathological Features of Ovarian Cancer in Rizgary Hospital/Erbil City from 2014 to 2017

Author: Marwa Jabbar Hussein, Jangi Shawkat Salai
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2019 Volume: 16 Issue: 2 Pages: 112-118
Publisher: Babylon University جامعة بابل

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Abstract

Background: Ovarian cancer is a heterogeneous disease with variations in clinical behavior and outcome. It is a group of many subtypes withdistinct biological features that lead to differences in response to treatments, recurrence rates, and survival. Objective: The aim of the studyis to describe the epidemiology of the diagnosed cases of ovarian cancer from 2014 to 2017 in Erbil, Iraq. Materials and Methods: This is aretrospective study, 100 ovarian cancer cases were reviewed from Rizgary Oncology Center in Erbil. Then, we compared histological typeswith age groups of the study as premenopausal and postmenopausal, stage of the disease, grade, and with side of tumor. Furthermore, this studyincludes the most important risk factors that may affect the incidence of ovarian cancer which includes menarche age, age of menopause, andwith obesity. Results: The result showed that ovarian cancer occurs in old age groups with a percentage of 64%. The most common type isepithelial ovarian cancer with subtype papillary serous adenocarcinoma in 56%, with all types, the most affected site is the right one in a percentof 57%. Grade 3 is the dominant one at presentation in a percent of 49%. About staging at the time of diagnosis, Stage 4 presented in a percentof 58%, and 16% of patients were obese. Conclusion: Most common type of cancer is epithelial type of papillary serous cystadenocarcinomahistology that occur in old patients and presented in advanced stage at the time of diagnosis with poor differentiation, and the most effectivetreatment is total abdominal hysterectomy + bilateral salpingo‑oophorectomy followed by adjuvant platinum‑based chemotherapy.


Article
Serum lipid peroxidation and trace elements levels in ovarian cancer patients before and after cisplatin and doxorubicin chemotherapy

Authors: Shatha A. Al-Shammaree --- Hathama R. Hasan --- Thikra H. Mathekoor
Journal: Journal of College of Education مجلة كلية التربية ISSN: 18120380 Year: 2011 Issue: 5 Pages: 119-136
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: chemotherapy and radiation therapy are associated with formation of reactive oxygen species and depletion of critical serum and tissue antioxidants. Although the oxidative statues in patients undergoing chemotherapy treatment were investigated in many studies, but their results were heterogeneous.Objective: This is a preliminary study conducted to explore the effects of cisplatin and doxorubicin on serum malondialdehyde levels (MDA) (as an oxidative stress indicator) together with the changes in levels of certain trace elements: Zinc (Zn), Copper (Cu), Manganese (Mn), and Iron (Fe) , Cu/Zn ratio, and protein concentration in ovarian cancer patients.Methodology: This is a case control study conducted in Chemistry Department/ College of Science / University of Baghdad. Six patients having histologically confirmed ovarian cancer(stage III) were participated in this study before and after chemotherapy with cisplatin and doxorubicin. Serum MDA was measured by Hirayama et.al spectrophotometry method, and the levels of certain serum trace elements: Zinc (Zn), Copper (Cu), Manganese (Mn), and Iron (Fe) were estimated by using flame atomic absorption spectrophotometry . Also serum protein concentration was measured in both mentioned groups. Three of our six patients were followed –up after the second course of the same chemotherapy where the same parameters were measured.


Article
Study of Estrogen, Progesterone, Copper, Zinc and some Antioxidants in Sera of Ovarian Cancer Patients
دراسة الاستروجين، البروجستيرون، النحاس، الخارصين وبعض مضادات الأكسدة في مصل دم مريضات سرطان المبيض

Author: Rana T. Ibrahim رنا طالب
Journal: Rafidain journal of science مجلة علوم الرافدين ISSN: 16089391 Year: 2013 Volume: 24 Issue: 5E Pages: 64-71
Publisher: Mosul University جامعة الموصل

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Abstract

The research was concerned with a study of the relationship between patients with ovarian cancer (OCa) and some biochemical parameters.Serum samples were collected from (25) healthy females and (25) females with ovarian cancer. The measured parameters in sera included the level of estrogen, progesterone hormones, some trace elements (copper and zinc), as well as some antioxidants such as glutathione, ceruloplasmin and malondialdehyde (MDA).Total protein was also measured in the present study. Compared with the healthy females, the females ovarian cancer exhibited a significant increase in the level of estrogen and progesterone hormones, ceruloplasmin, MDA and copper. They also exhibited a significant decrease in the level of glutathione, total protein and zinc.

تضمن البحث دراسة علاقة سرطان المبيض مع بعض المتغيرات الكيموحيوية. حيث جمعت نماذج من مصل الدم من (25) إمرأة من النساء السليمات و(25) إمرأة من النساء المصابات بسرطان المبيض. تضمنت دراسة المتغيرات الكيموحيوية على مصل الدم: تقدير مستوى هرموني الأستروجين والبروجستيرون، تقدير مستوى بعض العناصر النادرة (النحاس والخارصين)، قياس بعض مضادات الأكسدة الأخرى مثل الكلوتاثايون والسيريلوبلازمين كذلك التعرف على مستوى مادة المالونالديهايد. فضلا عن تقدير مستوى البروتين الكلي. أظهرت الدراسة وجود ارتفاعا معنويا في مستوى هرموني الأستروجين والبروجستيرون والسيريلوبلازمين والمالونالديهايد والنحاس، وكذلك انخفاضا معنويا في مستوى كل من الكلوتاثايون والبروتين الكلي والخارصين في النساء المصابات مقارنة مع النساء السليمات.


Article
MicroRNA-92gene expression in epithelial ovarian cancer using a novel Real-Time Polymerase change reaction

Author: Shoroq Mohammed AL-Temimi
Journal: Muthanna Medical Journal مجلة المثنى الطبية ISSN: 2226146x Year: 2016 Volume: 3 Issue: 1 Pages: 23-33
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

MiR–92 is one of six miRs encoded by the miR-17-92 cluster and its one of the best-characterized oncogenic miR clusters. A role for miR-92 in the pathogenesis of human cancers has been implicated by the high incidence of amplification in multiple neoplasm. The objective of this study is to estimation the miR-92 gene expression levels in both fresh tissues and serum ofsame ovarian cancer patients by using stem-loop follow by Taq-Man Real Time PCR (RT-PCR) technique and correlate the miR-92 gene expression with stages and lymph node involvement of ovarian cancer patients. Stem-Loop RT-PCR was performed to identify the level of miR-92 gene expression in both fresh tissues and serum of same ovarian cancer patients. The expression levels of miR-92 relative to mRNA of GAPDH were determined by using the livak method. Mean fold change of miR-92 was statistical significantly higher in ovarian cancer from precancerous tissues. Mean fold change of serum miR-92 gene expression was higher statistical significantly difference from healthy control , mean fold change of miR-92 in advance stage( III,IV) was higher statistical significantly difference from that early stage (I,II) and mean fold change of miR‑92 in patient with positive lymph node was higher statistical significantly difference from that of negative lymph node .There was no significant associationbetween gene expression of miR-92 and age of patients. Together we concluded that the over-expression of miR-92 were observed in serum patients with epithelial ovarian cancer compared with healthy controls, and miR-92 gene expression levels increased with lymph node involvement and advance stage of epithelial ovarian cancer.


Article
Expression and Clinical Significance of the Chemokine Receptor CXCR2 in Ovarian Cancer

Author: Maysaa G. Jumaah
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 70-76
Publisher: Baghdad University جامعة بغداد

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Abstract

The present study aimed to shed light on the role the of the Chemokine Receptor CXCR2 in the pathogenesis and progression of ovarian cancer. A total of 23 Paraffin-embedded tissue blocks from patients with different stages of newly diagnosed ovarian cancer were provided by certain Iraqi hospitals as well as 7 samples of patients with benign ovarian tumors tissues as a control group were used in this study. In the present study, the CXCR2expression was assessed by means of an envision immunohistochemistery technique using the NovolinkTM Polymer Detection Systems for both benign and malignant ovarian tumors. The results showed that 6(85.7%) of benign ovarian tumors and 22(95.7%) of ovarian cancer samples were positive for CXCR-2 which showed significant differences (P value0.048<0.5). CXCR2 was not expressed in 1(14.3%) of benign tumors and 1(4.3%) of ovarian cancer sections. Weak (+) CXCR-2 expression was observed in 2(28.5%) of benign tumors and 5(21.7%) of ovarian cancer sections which showed no significant differences. No significant differences were observed in the median CXCR-2 expression (++) between samples of benign tumors 3(42.8)% and samples of ovarian cancer 5(21.73%). The highest vascular signal intensity of CXCR-2 expression (+++) was observed in 1(14.3%) of benign tumors and 12(52.1%) of ovarian cancer sections, which showed high significant differences (P value **<0.01). In correlation with stages the results showed that18(94.44%) of samples with stage I and 4(100%) of samples with stage III were positive for CXCR2, which showed no significant differences (NS) with differences I signal intensity. In conclusion this study investigated that the percentages of sections with positive expression were higher in ovarian cancer tissue sections than the sections of benign ovarian tumors, and the signal intensity of staining was stronger in late stages of ovarian cancer tissue indicate role of CXCR2 expression in ovarian tumor progression, and maybe reveals the diagnostic value of this receptor for early diagnosis of ovarian cancer, and also provide the evidence for the ability of tumor cells to metastasize and then tumor angiogenesis and invasiveness.

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