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Article
Detection viral load of Parvovirus B19 in patients with Chronic Renal Failure

Authors: Sattar G. Ajeel --- Huda D. hathal --- Jawad K. mnate
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2016 Volume: 15 Issue: 3 Pages: 40-44
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background : Because of Parvovirus B19 that occur in patients with chronic renal failure ; Parvovirus B19 was found to be associated with or might be an aggravation factor of renal failure , we tried to find out the rate of occurrence of this virus in dialysis and non dialysis patients. Objective: The aim of the study was to investigate viral load for Parvovirus B19 was play an important role in the etiology of chronic renal failure patients Subjects and methods :A cross sectional study included Serum samples were collected from 50 dialysis patients and 50 without dialysis patients 50 normal subjects as control, and subjected for ELISA technique for detection of parvovirus IgG antibody, and multiplex Real time PCR for detection virus and viral load of this virus Results: Thirteen (26%) out of these 50 dialysis patients were positive by RT-PCR and, 16% (8/50) were positive by ELISA, while none of the controls was positive neither by RT-PCR nor by ELISA. The results of this study showed highly significant differences (p<0.001) on comparing between the median viral load in patients who had dialysis (2.38x106) copies / ml of Parvovirus B19 DNA and those who without dialysis(1.73x104) copies/ml of Parvovirus B19 DNA. Also the results of B19 by real time PCR analysis showed a significant difference (p<0.001) in median viral load in patients who had acute infection(2.87x106) copies/ml of Parvovirus B19 DNA ,than those who had chronic infection (2.29x105) copies/ml of Parvovirus B19 DNA , Conclusions Parvovirus B19 could be an important co-factor that play a role in CKD, which was higher rate in dialysis patients.


Article
تحديد الانواع الوراثية [P] و G لفيروس روتا الانسان في اطفال محافظة بابل باستخدام تقنية تفاعل البلمرة المتسلسل التقليدي conventional – PCR

Authors: يونس عبد الرضا --- حوراء جواد كاظم
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2017 Volume: 25 Issue: 1 Pages: 81-88
Publisher: Babylon University جامعة بابل

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Abstract

Rapid test device technique and conventional - PCR technique was used in this study for detection of rotavirus genotypes in stool samples which were collected from children who were living in different area of Babylon governorate with symptoms of acute gastroenteritis such as diarrhoea , fever and vomition for the period from October 2011 to March of 2012 , Their age range from 0 – 60 months , 470 diarrhoea stools sample from children was tested by using rapid stripe , then 80 sample which gave positive result by rapid test device were used to know G and [P] type by using specific G and [P] primer .After extraction of viral RNA and its convertion to cDNA . The results detected that the prevalent G – genotypes was G1 , G4 , G2 and G8 respectively , whereas P[8] , P[4] and P[6] was the most dispersal P – genotypes .

استعملت في هذه الدراسة تقنية الشريط السريع و تقنية تفاعل البلمرة المتسلسل المعكوس التقليدي (conventional reverse transcriptase – PCR) بغية معرفة الأنواع الوراثية لفيروس الروتا في عينات براز أخذت من أطفال مخمجين من مناطق متفرقة في محافظة بابل , أظهروا بعض الأعراض المُميزة لالتهاب المعدة والأمعاء الحاد مثل الإسهال والحمى والتقيؤ للمدة من تشرين الأول و تشرين الثاني و كانون الأول من العام 2011 وكانون الثاني و شباط و آذار من العام 2012 , تراوحت أعمارهم من 0- 60 شهراً, إذ اختبر 470انموذجا من عينات براز الاطفال باستعمال الشريط السريع , وبعدها اخذ 80 نموذجا اعطى نتيجة موجبة باستعمال الشريط السريع لمعرفة الانواع الوراثية السائدة وباستعمال بوادئ خاصة Specific primers للأنواع الوراثية P]] و G وبعد استخلاص الحامض النووي الريبي RNA لفيروس الروتا من النماذج وتحويله إلى الدنا المتمم complementary DNA وجد أن أكثر الأنواع الوراثية المشخصة من النوع G انتشاراً في المحافظة هي G1 و G4 و G2 و G8 , بينما لوحظ أن الأنواع P[8] و P[4] و P[6] هي الأكثر انتشاراً بالنسبة للنوع الوراثي P]] .

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