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Effect of Trichothececns toxin on stem cells isolated from Umbilical cord blood

Author: Hebat Alla Adel Abdulla AL-Hamdani1 , Mohammed Q.AL-Annie1 , Salah M.A.AL-Kubaisi2
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2018 Volume: 17 Issue: 3 Pages: 100-111
Publisher: Baghdad University جامعة بغداد

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Abstract

The study included the collection of umbilical cords blood samples immediately after delivery . Hematopoietic stem cells were isolated from the umbilical cord blood by the gradient density method. The hematopoietic stem cells (MNCs) were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10 % of fetal calf serum, then subjected to in vitro study to confirm the toxic effects of Trichothecenss extracts with the following concentrations (0.001,0.0039,0.0078, 0.0156, 0.031, 0.062, 0.13, 0.25, and 1 μg/ ml) and to detect the inhibition rate of hematopoietic stem cells by using 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. The cytotoxicity assay (in vitro study), revealed that the inhibition rate increases seriously with the increase of Trichothecens concentration. Hence the concentration 1 μg /ml has given inhibition rate which reached 100%, while the concentration 0.001 μg /ml did not eliminates any type of cells. Trichothecens has a highly toxic effect on human hematopoietic stem cells, and its concentration is high and positively associated with the rate of cell death increases. Furthermore, the concentration 1 μg /ml of Trichothecens extracted from the Trichothecensgenic isolates can kill 100% of hematopoietic stem cell.

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