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Article
Extraction and Evaluation the Activity of Urtica dioica as Bleeding Stop Material

Author: Hashim M. Zehraw , Zynab Muotaz , Muamer Taleb , Bydaa Mukhlif , Saad Abed Al-Jabbar
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2019 Volume: 18 Issue: 2 Pages: 77-87
Publisher: Baghdad University جامعة بغداد

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Abstract

Urtica dioica From the medicinal herbs group, with many uses, the extract of the plant was prepared in order to study effect on the process of stopping hemorrhage of various pathological causes, collection of plants in the pre-blooming phase of the Jadiriyah area, Baghdad, Iraq. Prepare a sample of it by way of alcohol extraction for all parts of the plant. The substances and active groups were identified using chemical analysis. The results showed the presence of the necessary phytol in the blood clotting process as well as the glycosides, tannins, proteins, flavonoids and others. Some antioxidant, anti-inflammatory and anticancer compounds were found using gas chromatography technique. High-performance liquid chromatography was used as the important morphine was identified in the manufacture of a number of vitamins. The trace sample showed a high concentration of sodium and iron and a low concentration of zinc. The tested sample passed the toxicity test carried out on laboratory animals and proved its non toxic in the doses used. The results of the treatment of blood hemorrhage in mice found that the plant extract had an effect on reducing the duration of bleeding cessation to 1.14 seconds compared to the non-treated group (3.21) seconds, about 60% less, the hemoglobin level in rabbits was reduced to a significant level (P <0.01) to 13.2 g / dl compared with the control (14.5) g / dl and decreased packed cell volume (PCV) to 41 ml compared to 46 ml with control. It is noted that the number of white blood cells decreased with a significant value (P <0.01) to 5900 × 10 3 μl compared with control 8200 × 10 3 μl and found a decrease (P <0.01) at the time of bleeding by 50% after treatment with the extract and reduced the coagulation time by one third and with a significant value P <0.01).

Keywords

Urtica dioica --- Hemorrhage --- phytol --- Morin.


Article
EFFECTS OF ( Artemisia herba-alba) AND (Urtica dioica) ON SOME BLOOD PARAMETERS IN BROILER CHICK WHICH INFECTED EXPERIMENTALLY WITH Eimeria tenell
تأثير عشبتي الشيح والقريص على بعض المعايير الدمويه في فروج دجاج اللحم المخمج بطفيلي الايمريا تنيلا

Author: *Dunya A.M. Mohammad **Haider M. Ali *** Amal H. Atyha
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2016 Volume: 15 Issue: 1 Pages: 278-291
Publisher: Basrah University جامعة البصرة

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Abstract

This study was carried out to determine the effect of the herb and Urtica dioica of the vitality of the Eimeria tenella, it conducted on 150 Ross broiler chicks, divided randomly on the seventh days into six groups. The last two groups (five and six) were considered as a positive and negative control groups, the first five groups were experimentally infected by 1000 oocysts/bird of Eimeria tenella at 17 days old directly in to the crop. The first four groups were given Artemisia herba alba and Urtica dioica According to concentrations for each group. Results were indicated a higher significant differences ( P<0.05 ) in the means of PCV for the groups that treated water herbs extracts of Artemisia herba alba and Urtica dioica (38.5±1.3 ,39.5±1.6%) respectively compared to positive and negative control groups (32.0 ±1.0, 33.0±1.13%) ; also , increase in the means of Hb concentration in the groups which treated with the Urtica dioica in food (14.2 ± 1.1 gm/100ml) and water extract (15.4 ± 1.2 gm /100 ml ) respectively compared to the control groups. A higher significant differences (P<0.05) were recorded in means of WBCs of the water extract of both herbs Artemisia herba alba and Urtica dioica (30.8 ± 0.3 ,31.0 ± 0.3 x103cell/ml) respectively compared to control group. While the differential count of WBCs were showed a significant decrease (P<0.05) in the heterophils of both herbs of water extracts of Artemisia herba alba and Urtica dioica (20.4±1.5% , 19.0±1.7%) respectively, especially at 5th week compared to control positive and negative groups. In conclusion used herb Urtica dioica especially aqueous extract enhance immune efficiency as well as the aqueous extract of the Artemisia herba-alba herb. And use the standard doses of 1% or less from Artemisia and Urtica dioica fodder and water are safety doses without mortality or side effects in broiler. Further studies are needed to (re) evaluate effect of Urtica dioica, Artemisia on other parasites.


Article
The Clinical and Hematological changes in rabbits exposed to leaves of Urtica dioica under experimental conditions
التغيرات السريرية والدموية في الأرانب المعرضة على أوراق نبات القريص ( Urtic dioica ) تحت الظروف التجريبية

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Abstract

Abstract: The study was conducted on 20 female local breed rabbits , of 1-2 years age , of 1- 2 kg body weight . In first part of the study , 10 animals were randomly divided into two groups of 5 each. The first group was exposed to leaves of Urtica dioica in green phase at a dose rate of 50 grams / kg b.w daily for 10 days , while those of the second group left without exposure as a control group . In the second part of the study , animals of the first group exposed to leaves in dry phase , at a dose rate of 5 g/kg b.w. daily for three weeks, as a powder mixed with the concentrated food. While those of the second group left as a control group without exposure. The main dependent parameters in the study were ,clinical parameters ( body temperature , heart beat , respiratory rate, body weight , in addition to monitor any abnormal signs appear on the animals) . The main hematological parameters which depended in the study were included , RBC count , WBC count , Hb concentration , PCV percentage , Red cell indices , Bleeding time and Clotting time . The results of the study revealed the following : In first part clinically ; some rabbits showed strong heart beat , in other heart beat decreased ; respiratory rate decreased with noisy vesicular murmurs were heard . Body weight and body temperature were decreased. In the fourth day post exposure to plant one rabbit showed softy feces . In the 16th day post exposure one animal died , followed by another on in 18th day of the experiment . The post mortem findings were pale mucous membranes , stomach impacted with food with ulceration of mucus membranes . severe congestion of liver and gastrointestinal tract . sever swelling of kidney , urinary bladder filled with urine of dark yellow color .Hematologically clotting time prolonged , with decrease PCV , and White blood cells counts ; while Hb concentration did not showed any changes In second part: Heart beats , respiratory rates and body temperature were increased , while body weight decreased . Prolongation of clotting and bleeding time .The hematological pictures revealed that , Hemoglobin level and PCV values were decreased .The total erythrocyte count did not showed any significant changes during the study . The mean corpuscular volume and mean corpuscular hemoglobin ,the mean corpuscular hemoglobin concentration were significantly decreased .The total leucocytes count ,the percentage of heterophils, and monocytes percentage were increase. The lymphocytic and, esinophils percentage were significantly decreased .The basophiles values did not showed any significant changes during the study.

الخلاصة : شملت الدراسة والتي أنجزت على 20 أنثى من الأرانب المحلية ، بعمر 1-2 سنة ، ووزن 1- 2 كغم على جزئين ، 10 أرانب في كل جزء. قسمت في كل جزء إلى مجموعتين ؛ عرضت المجموعة الأولى على أوراق النبات الخضراء وبجرعة 50غم كغم ، يوما لمدة 10 أيام . ، وتركت المجموعة الثانية بدون تعرض كمجموعة سيطرة. في الجزء الثاني عرضت المجموعة الأولى على مسحوق أوراق النبات الجافة بواقع 5 غم كغم ،يوما لمدة ثلاث أسابيع. شملت المعايير الرئيسية المعتمدة على التغيرات السريرية والتي تضمنت حرارة الجسم ، وزن الجسم ، معدل التنفس وضربات القلب . أما المعايير الدموية فتضمنت على عد خلايا الدم الحمر ، وتركيز خضاب الدم ، حجم الخلايا المرصوصة ، معدل حجم الخلية ، معدل خضاب الخلية ، ومعدل تركيز خضاب الخلية . فضلا عن زمن التخثر والنزيف . أظهرت نتائج الدراسة : في الجزء الأول سريريا ؛ أظهرت بعض الحيوانات ضربات قلب قوية ، وفي الأخرى هبط عدد ضربات القلب .معدل التنفس هبط مع سماع أصوات نفحية ضوضائية في البعض, هبوط حرارة الجسم ووزن الجسم . في اليوم الرابع بعد التعرض ظهر براز لين . هلك احد الحيوانات في اليوم السادس عشر وأخر في اليوم الثامن عشر. التغيرات في الصفة التشريحية كانت شحوب الأغشية المخاطية، المعدة متخمة بالغذاء، تقرح الأغشية المخاطية، احتقان شديد للكبد، والقناة الهضمية. تورم شديد للكلى ، المثانة ممتلئة بالبول الأصفر الداكن . شملت التغيرات الدموية على إطالة التخثر ، مع هبوط حجم الخلايا المرصوصة ، وعدد الخلايا البيض : تركيز الخضاب لم تظهر تغير . في الجزء الثاني : زيادة ضربات القلب ، معدل التنفس ، وحرارة الجسم . وهبوط الوزن . إطالة زمن النزف والتخثر . أظهرت التغيرات الدموية أن عدد خلايا الدم البيض ارتفع ، المختلفات ارتفعت ، بينما مستوى الخضاب هبط وحجم الخلايا المرصوصة . أعداد خلايا الدم الحمر لام تظهر تغير معنوي .معدل حجم الكريه ومعدل خضاب الكريه ومعدل تركيز خضاب الكريه هبطت . أعداد خلايا الدم البيض ارتفعت ، ونسبة المختلفات ارتفع الخلايا الأحادية ارتفعت ، نسبة اللمفاوية هبطت ، والحمضات هبطت ، القعدات لم تتغير.


Article
Effect of alcoholic extract of urtica dioica leaves and Aspirin in experimentally induced prostatitis in mice

Author: Huda F. Hasan
Journal: Kufa Journal For Veterinary Medical Sciences مجلة الكوفة للعلوم الطبية البيطرية ISSN: 20779798 Year: 2014 Volume: 5 Issue: 2 Pages: 230-240
Publisher: University of Kufa جامعة الكوفة

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Abstract

Urtica dioica leaves were powdered and extracted with methanol 70 %. 17 beta-estradiol was used to induce prostatitis. Fifty mice were divided into five groups, The first control group (T1) , The second induction group (T2) was treated by distilled water, the third induction group (T3)was treated with crude extract of Urtica dioica leaves 300mg/kg B.W, the fourth induction group (T4) was treated with aspirin 50mg/kg B.W, the fifth induction group (T5)was treated with crude extract of Urtica dioica leaves 150mg/kg B.W and aspirin 25mg/kg B.W. Superoxide dismutase (SOD) and glutathione peroxidase (GPx) for induction groups treated with 17 beta-estradiol showed significant decrease as compared with (T1), while the T3,T4 and T5 showed no significant difference as compared with T1. The result of testosterone hormone of (T3) showed significant increase as compared with (T1) and other groups. The results of body and prostate weight for T3,T4 and T5 revealed no significant difference as compared with T1. The fertility index of (T3) was increased as compared with T1 while the T4 was decreased as compared with T1. The histopathological changes after induction of prostatitis revealed hyperplasia of the prostatic glands and infiltration of inflammatory cells, while the T3 revealed the return of the acini to normal shape, and hydropic degeneration in the epithelium of prostatic glands disappeared. The T4 showed decreased hydropic degeneration, few inflammatory cell and epithelial hyperplasia, while the T5 was characterized by few inflammatory cell infiltration and the acini showed very decreased hydropic degeneration. In this study we concluded that the alcoholic Urtica dioica extract could markedly treat proststitis by its anti-inflammatory effect with reduce side effect of aspirin such as infertility.


Article
Antibacterial effect of nettle (Urtica dioica)
التأثير المضاد للبكتيريا لنبات القريص

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Abstract

The antibacterial activity of aqueous and 95% ethanol extracts of nettle leaf were tested against some Gram-negative and Gram-positive bacteria isolated from hospitalized patients by the agar well diffusion method. Staphylococcus aureus, Escherichia coli, Klebsiella spp., Bacillus Subtillus, Proteus spp. Salmonella spp. and Pseudomonas spp. were used. The results indicate that both extracts showed different antibacterial activities which was in favor of ethanolic extract because of more solubility of active ingredient in ethanol than in water. Staphylococcus aureus, Bacillus Subtillus and Salmonella spp. showed the highest susceptibility to nettle extracts antibacterial effect, while E coli, Pseudomonas and Proteus were less susceptible. The only clear resistant bacteria isolate was Klebsiella spp.

تمت دراسة التأثير المثبط للخلاصة المائية والكحولية (الإيثانول 95%) لنبات القريص مكون من الأوراق ضد نمو أنواع من البكتريا الموجبة والسالبة لصبغة غرام, لعزلات جرثومية من المرضى في المستشفى بطريقة الانتشار في بالحفر. البكتريا المستعملة هي المكورات العنقودية الذهبية و الاي كولاي و زوائف الايروجينوزا و الكليبسيلا والسالمونيلا وباسيلص سبتيلص و بروتيس , أظهرت النتائج بان للخلاصتين تأثيرات مختلفة كمضاد بكتيري ولكن النتائج كانت بأفضلية للمستخلص الكحولي بسبب قابلية الذوبان الأكبر للمكونات العشب في الكحول التي لا تذوب في الماء.الفاعلية التثبيطية لمستخلص العشب كانت اكبر ضد االمكورات العنقودية الذهبية والسالمونيلا وباسيلص سبتيلص بينما كانت الفاعلية التثبيطية لمستخلص العشب ضد الاي كولاي و زوائف الايروجينوز و بروتيس قليلة , الكليبسيلا كانت مقاومة جدا للعشب و لم تظهر اي نتائج متعلقة بالفاعلية التثبيطية لمستخلص العشب


Article
Effect of Artemisia herba alba and Urtica dioica herbs extracts in the biological of Eimeria tenella parasite in broiler chicks.
تأثير مستخلصات عشبتي الشيح Artemisia herba alba والقريص Urtica dioica في مستوى التبوغ لطفيلي Eimeria tenella في المختبر

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Abstract

This study was conducted to determine the biological effects of both Artemisia herba alba and Urtica dioica on the percentage of sporulation of Eimeria tenella parasite in laboratory. The results were revealed that the percentage of the sporulation for oocysts parasite in groups treated with alcoholic and aquatic extracts of Artemisia herba alba concentrations 0.1%, 0.5% and 1% reduced up to 60% 65%, 68%, 64%, 70% and 75%, respectively. Also the alcoholic and aquatic extracts of Urtica dioica were reduced the percentage of sporulation for up to 62%, 64%, 70%, 68%, 72% and 75%, respectively, when compared with the control positive group, which amounted to 90% after 96 hours.

هدفت هذه الدراسة إلى معرفة تأثير مستخلصات عشبتي الشيح ) Artemisia herba alba ( والقريص ) (Urtica dioicaعلى نسبة تبوغ طفيلي ال Eimeria tenella في المختبر فقد انخفضت نسبة التبويغ لأكياس بيض الطفيلي في المجاميع المعاملةبالمستخلصات الكحولية والمائية لعشبة الشيح بالتراكيز %0.1 و %0.5 و %1 لتبلغ %60 و 65 % و %68 و %64 و 70 %و 75 % على التوالي، كذلك أثرت المستخلصات الكحولية والمائية لعشبة القريص في خفض نسبة التبوغ لتصل الى %62 و %64و %70 و %68 و %72 و %75 على التوالي مقارنة مع مجموعة السيطرة والتي بلغت %90 بعد مرور 96 ساعة.


Article
Effect of Urtica Dioica Leaves and Moringa oleifera Leaves and Seeds in Anemia in Males Rats Exposed to Cadmium Chloride and Lead Acetate Poisoning.
تأثير اوراق القريص واوراق وبذور المورنجا في فقر الدم لذكور الجرذان المعرضة للتسمم بكلوريد الكادميوم وخلات الرصاص.

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Abstract

The current study was designed to observe and test the preventive and Therapy effects of Urtica dioica leaves and Moringa oleifera leaves and seeds powder on healthy adult albino rats males and that exposed to cadmium chloride (5 mg/kg of body weight) and lead acetate (50 mg/kg of body weight) and the amount of the transaction over the period (30) days on case of anemia, randomly divided into (18) groups, and each group included (5) animals. Giving the powder of Urtica dioica leaves and Moringa oleifera leaves and seeds showed on significant differences (p˃0.05) in the weight of the organs, which include liver, kidney and spleen,WBCs, RBCs, Hb, PCV, MCV, MCH and MCHC in comparison with the normal control group. The groups that had been given cadmium chloride and lead acetate caused a significant decrease in (P˂0.05) RBCs, Hb, PCV and a significant increase in the weight of the organs(liver, kidney and spleen),WBCs, MCV and MCH, while observe no significant differences in MCHC. The groups of animals which treated with powder of these plants under study before and after of exposed to each of cadmium chloride and lead acetate showed a positive effect on the weight of the organs (liver, kidney, spleen) and showed led to a significant increase in RBCs, Hb, PCV, and a significant decrease in WBCs, MCV and MCHC, all of these were compared with the groups that exposed to cadmium chloride and lead acetate only.

صممت التجربة لملاحظة واختبار التأثيرات الوقائية والعلاجية لمسحوق (اوراق القريصUrtica dioica ، اوراق وبذور المورنجا Moringa oleifera) في ذكور الجرذان البيض السليمة والمعرضة لكلوريد الكادميوم (5 ملغم/كغم من وزن الجسم) وخلات الرصاص (50 ملغم/كغم من وزن الجسم) وطيلة فترة المعاملة البالغة (30) يوماً على حالة فقر الدم، وزعت الحيوانات إلى ثماني عشرة (18) مجموعة ضمت كل مجموعة خمسة (5) حيوانات وبأوزان متقاربة، أن اعطاء مسحوق اوراق القريص واوراق وبذور المورنجا ادى الى عدم حصول أختلاف معنوي P˃0.05)) في وزن الاعضاء التي تشمل كل من الكبد، الكلى والطحال وعدد خلايا الدم البيض(WBCs) ، عدد كريات الدم الحمر RBCs))، تركيز خضاب الدم (Hb)، حجم خلايا الدم المرصوصة PCV))، متوسط حجم الكرية الحمراء(MCV) ، متوسط خضاب الكرية الحمراء(MCH) ، متوسط تركيز خضاب الكرية الحمراء(MCHC) مقارنةً مع مجموعة السيطرة السليمة. وان مجاميع الحيوانات التي اعطيت كلوريد الكادميوم وخلات الرصاص ادى الى حصول انخفاض معنوي في عدد كريات الدم الحمر، تركيز خضاب الدم، حجم خلايا الدم المرصوصة وزيادة معنوية P˂0.05)) في اوزان اعضاء (الكبد، الكلى والطحال)،WBCs، MCV، MCH، بينما لم يلاحظ وجود اختلاف معنوي في MCHC. اما مجاميع الحيوانات المعاملة بمسحوق تلك النباتات قبل وبعد التعرض لكل من كلوريد الكادميوم وخلات الرصاص اظهرت تأثير ايجابي على وزن اعضاء(الكبد، الكلية والطحال) واظهرت زيادة معنوية في RBCs، Hb، PCV وانخفاض معنوي فيWBCs ، MCV، MCH. مقارنة مع المجاميع المعرضة لكلوريد الكادميوم وخلات الرصاص.

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