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Article
P53 expression in colonic carcinoma – immunohistochemical study

Authors: Bedoor AK. Al-Irhayim بدور عبد القادر الارحيم --- Dena A. Jerjees دينا اكرم جرجيس
Journal: Annals of the College of Medicine Mosul مجلة طب الموصل ISSN: 00271446 23096217 Year: 2009 Volume: 35 Issue: 2 Pages: 111-116
Publisher: Mosul University جامعة الموصل

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Abstract

ABSTRACT
Objectives: To evaluate p53 protein expression in colorectal tumors in Mosul city and correlates it with various clinicopathological parameters and to compare the results with other studies.
Patients and methods: This is a pro and retrospective study of 53 samples of adenocarcinoma of colon with age range from 5 to 80 years. There were 38 males, with age range (5 to 80) years and 15 females with age range (18 to 70) years. The samples were collected from Al-Jumhuri Teaching Hospital, Nineveh Private Hospital and private labs during the period from September 2007 through May 2008. P53 protein was detected immunohistochemically by using the primary antibody (monoclonal antibody clone (DO-7¹) and ™ G|2 visualizing system/AP, Rabbit/Mouse (permanent red). Positive and negative controls were included in each run. The interpretation was done by a semi quantitative method.
Results and conclusions: P53 protein expression was found in 49% (26 cases) of colorectal cancer.
There was no correlation of p53 expression with age, sex, site, and size, but was significantly correlated with grade and stage (p value =0.001,0.044, respectively). By stepwise backward multiple logistic regression the grade was the only independent factor (p value =0.033).
Keywords: P53, colonic adenocarcinoma.

الأهداف: لتقييم إظهار بروتين p53 في أورام القولون في مدينة الموصل والربط بينها وبين مختلف الصفات المرضية السريرية للورم ومقارنة هذه النتائج مع نتائج دراسات أخرى.الحالات والطرق: هذه الدراسة مستقبلية و رجعية فيها 53 حالة لسرطان القولون بأعمار تتراوح من 5-80 سنة. لقد كان عدد حالات الذكور 38 وبأعمار تتراوح (5-80 سنة) وكان عدد حالات الإناث 15 وبأعمار تتراوح (18-70) سنة. لقد تم جمع النماذج من مستشفى الجمهوري التعليمي، مستشفى نينوى الأهلي والمختبرات الخاصة خلال فترة امتدت بين شهري أيلول 2007 خلال أيار 2008. لقد تم التحري عن بروتين p53 بطريقة مناعية-نسيجية-كيميائية باستخدام المضاد الأولي (المضاد أحادي النسل نوع دي أو 7^1) ونظام إظهار نوع المعلم تي ام جي2أي ﭙي، أرنب فأر (أحمر دائم). لقد تم استخدام الضوابط الموجبة والسالبة مع كل وجبة وقد تم تحليل النتائج بطريقة شبه كمية.النتائج والاستنتاجات: لقد وجد بروتين p53 في 49% (26 حالة) من حالات سرطان القولون. لم يكن هناك أي علاقة ربط لإظهار بروتين p53مع العمر، الجنس، موقع وحجم الورم. ولكن كانت هناك علاقة معنوية مع مرتبة الورم و مستوى تقدمه (٫001 0 =Р، 0٫044=Р) . بالعلاقة الرجعية متعددة المنطق متدرجة الرجوع كانت مرتبة المرض هي العامل الوحيد المستقل (0٫033 =Р).


Article
Skeletal Metastasis;Distribution by Age, ,Sex,and Histological Classification(Medical city)

Author: Abdul Ridha Kh.Jabbar
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 2 Pages: 192-195
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

BACKGROUND: Skeleton is the one of the commonest sites of secondary cancer, it is seen more frequently than all primary bone tumors together. Vertebrae, pelvis and proximal parts of long bone are moreinvolved by secondary metastasis. Breast cancer is the commonest cause of primary tumorfollowed by Prostatic carcinoma. Despite the advances in diagnosis but still about 10 % ofcases remain as secondary with undetermined primary site.OBJECTIVE: To disclose the distribution of bone metastasis and their primary site among Iraqi patients. PATIENTS AND METHOD: 102 patient were enrolled in a retrospective study conducted in medical city complex inBaghdad/ Iraq from January 1990- July 2000.Patient are either known to have primary malignant tumor and discovered to have secondarymetastasis to bone as part of the routine screen or development of symptoms suggestive ofbone metastasis, the other group of patients are presented with bone metastasis. All patientsare subjected to intensive medical history and proved their secondary metastasis by excisionalbiopsy or fine needle aspiration with histopathological diagnosisRESULTS: The mean age of our patients was 41 years, with range (2-80 years), men were commonlyinvolved than women, the peak incidence of metastasis was in 1991. Vertebral bodies arecommonly involved and lungs are the most common primary siteCONCLUSION: Well differentiated columnar adenocarcinoma is the commonest histopathological type ofmalignant tumor metastases to bones, lungs are the most common primary site. Undeterminedprimary tumors are still consist a diagnostic problem in our daily practice more cooperativeteam work is needed to disclose the final diagnosis


Article
COEXPRESSION OF HER2 AND P53 IN GASTRIC AND ESOPHAGEAL ADENOCARCINOMA
معاينة HER2 و P53 في السرطان الغدي للمعدة والمرئ

Authors: INTISAR SALIM PITY انتصار سالم بتي --- SARDAR HASSAN ARIF سردار حسن عارف --- HAYDER HUSAIN IBRAHIM حيدر حسين ابراهيم --- GINA GAMES GEORGE جينا جمس جورج
Journal: Duhok Medical Journal مجلة دهوك الطبية ISSN: ISSN: 20717334 (online)/ ISSN: 20717326 (Print) Year: 2015 Volume: 9 Issue: 2 Pages: 60-73
Publisher: University of Dohuk جامعة دهوك

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ABSTRACTBackground and objectives: Gastric and esophageal adenocarcinoma remains deadlydiseases with an on rise incidence. The recently discovered cancer-related molecular markers,such as HER2 and p53, help facilitate response to preoperative therapy and improve overallsurvival. This study was aimed to detect the immunoexpression of HER2 and p53 in gastricand esophageal adenocarcinoma and to determine the association of these two markers withclinicopathological parameters.Method: The study was conducted in the Central Laboratory and Directorate of Health,Duhok-Iraq during a period from May 2009 to September 2014 on 101 gastric andesophageal adenocarcinoma cases. Using monoclonal antibodies against HER2 receptors andp53 nuclear protein, slides were stained with the fully automated immunostaining instrument,Ventana Benchmark.Results: Total positive HER2 immunoexpression was demonstrated in 33.7% of cases with asignificantly higher dense HER2 (3+) expression in esophageal adenocarcinoma comparedwith its gastric counterpart. p53 nuclear staining was observed in 62.4% of cases; it wassignificantly higher in gastric cancer than esophageal adenocarcinoma. HER2 was limited tothe intestinal type whereas p53 was found to be expressed in both intestinal and diffuse types.No significant coexpression was demonstrated between HER2 and p53 in any of gastric oresophageal adenocarcinoma.Conclusions: HER2 expression was limited to the intestinal type gastric adenocarcinoma. Nosignificant coexpression of HER2 and p53 was demonstrated in both of gastric andesophageal adenocarcinoma.

معاينة HER2 و P53 في السرطان الغدي للمعدة والمرئ


Article
Photoimmunotherapy by Cortactin monoclonal antibody conjugated with Hematoporphyrin derivative of a subcutaneous murine mammary adenocarcinoma using low power He-Ne laser
العلاج المناعي الحركي الضوئي للخلايا السرطانية باستعمال الاجسام المضادة الاحادية النسيلة المرتبطة بالمحسس الضوئي

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Abstract

Photoimmunotherapy (PIT) is a promising approach which aims to increase the specificity of photosensitisers used in photodynamic therapy through its conjugation to monoclonal antibodies (MAb) directed against tumour antigens. The current study aims to increase and improve the therapeutic selectivity of photodynamic therapy (PDT) by conjugating the HpD to specific (mAb) in order to target one of the specific antigens of mammary adenocarcinoma. a screening has been done to detect specific antigens on mammary adenocarcinoma cells and we found that Cortactin is the unique antigen on tumor tissue in our model, then anti-cortactin mAb was linked to HPD, used for not only improve specificity, but vastly enhances tumor cell killing when they are photo-immunotargeted by HpD-monoclonal antibody conjugates comparing with unconjugated HPD and then exposed to low power He-Ne laser (632 nm). Female mice, which were transplanted with AM3 (mouse mammary adenocarcinoma transplantable tumor line), were randomly divided into five groups of 10 mice each; the first group was treated by PIT using HpD conjugated with Cortactin, in comparison with HpD alone in a second group. The third and fourth were control groups with and without HpD alone respectively. Tumor growth indices and metastasis incidence were calculated. Photo-immunotargeting by HpD-mAb conjugates activated by a low-power He-Ne laser showed significant tumor growth inhibition similar to PDT using unconjugated HpD activated by He-Ne laser, whilst control groups were without any significant effect. The resultssuggest that conjugation was with low quantity of the HPD or not stable enough and this need further studies.

يعد العلاج المناعي الضوئي طريقة واعدة تهدف لزيادة انتقائية المحسسات الضوئية المستعملة في العلاج الحركي الضوئي من خلال الاقتران مع جسم مضاد احادي النسيله والذي يوجه ضد مستضد ورمي. هدفت الدراسة الحالية الى زيادة وتحسين الانتقائية العلاجية للعلاج الحركي الضوئي من خلال اقتران مشتقات الهيماتوبيرفورين مع جسم مضاد احادي النسيله يستهدف مستضد معين خاص بسرطانة الغدة اللبنية. تم اجراء مسح للمستضدات الخاصة بسرطانة الغدة اللبنية ووجد ان الكورتاكتين هو مستضد فريد على النسيج الورمي في نموذجنا المدروس. تم اقتران الجسم المضاد مع مشتقات الهيماتوبيرفورين لتحسين الانتقائية وبالتالي قتل الخلايا السرطانية عندما يتم استهدافها بالجسم المضاد المقترن مع المحسس الضوئي وتعريضها بعد ذلك لليزر ذو القدرة الواطئة. تم غرس فئران اناث بورم الغدة اللبنية الفاري ووزعت عشوائيا على خمس مجاميع من عشرة فئران, تم معالجة الاولى بالمحسس المقترن والليزر في حين لم تعرض المجموعه الثانية لليزر وجرعت المحسس المقترن فقط. المجموعه الثالثة اعطيت فقط محسس والرابعه تركت كمجموعة سيطرة. تم قياس نمو الورم ووجد ان للمحسس المقترن تاثير قوي مشابه لغير المقترن وان لم يكن اكثر تاثيرا وذلك ربما يعزو لقلة المحسس المرتبط او لعدم استقرار الارتباط وهذا يحتاج الى دراسات اكثر.


Article
The Prognostic Role of (CD34) In The Angiogenesis of Endometrial Adenocarcinoma

Author: Lubab Fadhil Talal
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 1 Pages: 77-86
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND: Tumor angiogenesis is essential for tumor growth and metastases. Recently ,studies showed that microvessel density (MVD),a measure of tumor angiogenesis has found to have a prognostic significance for predicting metastases and survival in endometrial adenocarcinoma and identifying patients at high risk for recurrences . The aim of this study is to understand the correlation between the MVD and various clinicopathological factors in endometrial adenocarcinoma in order to evaluate the role of angiogenesis in the assessment of the biological behavior of endometrial adenocarcinoma and to prove that MVD can be used as a valuable prognostic factor and can detect high risk patients for post-operative recurrence who may need adjuvant therapy after surgery . PATIENTS AND METHODS: Forty-one (41) cases were included in this study ; one case is normal endometrium in the proliferative phase , 5 cases with sever endometrial hyperplasia and 35 cases with endometrial adenocarcinoma taken from the Pathology Department at the Medical City and Private Pathology Laboratory between the years 2000-2004 . Paraffin blocks cut in two sections ; one for Haematoxylin & Eosin stain and the other for CD34 immunostaining slides were studied well for tumor grade ,stage ,myometrial invasion and pelvic and paraaortic lymph nodes metastases . The mean MVD was studied by scanning the tumor sections at a low power X40 and X100 then identifying areas of highest neovascularization (hot spots) .Five hot spot areas were selected from each section ,and the MVD were counted at X200 magnification (x10 ocular ,x20 objective) by taking the mean of those microvessels. The correlation between mean MVD and various clinicopathological factors were studied including the age ,tumor grade ,stage ,myometrial invasion and pelvic and paraaortic lymph nodes metastases . RESULTS : CD34 is a reliable and sensitive endothelial cell marker used in the assessment of tumor angiogenesis The study showed that a significant statistical correlation was found between the mean MVD and patients age , tumor grade and myometrial invasion. While there were no statistical correlation significance between the mean MVD and tumor stage and pelvic & paraaortic lymph nodes . CONCLUSION : We conclude that MVD is important in the assessment of the biological behavior of endometrial adenocarcinoma , where it is an indicator for the emergence and growth of tumor and it is important to detect patients at high risk of recurrences and to decide the post-operative adjuvant therapy


Article
PHOTODYNAMIC THERAPY OF SUBCUTANEOUS MURINE MAMMARY ADENOCARCINOMA
العلاج الحركي الضوئي بالحقن داخل ورم سرطان الغدد

Authors: Ahmed M. H. Al-Shammari2 --- Ahmed S. K. Al-Khafaji1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2012 Volume: 11 Issue: 1 Pages: 109-120
Publisher: Baghdad University جامعة بغداد

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ABSTRACTPhotodynamic therapy (PDT) of cancer is a treatment based on the accumulation of aporphyrin-related photosensitizer in tumor cells, and their subsequent destruction based onexposure to light source of specific wave length. Hematoporphyrin derivative has been shown aselective localization in malignant tissues and causing their destruction by generated singletoxygen when it's activated by appropriate wavelength (λ) of irradiation. Singlet oxygen speciesare produced and then caused membranes' and organelles' damage leading to cell death andtumor ablation. In this study, Female mice transplanted with AM3 (mouse mammaryadenocarcinoma transplantable tumor line) randomly divided into four groups of 10 miceeach, the mice of first group were intratumorally injected with 30 mg HPD/kg of body weightand exposed to 10 minutes of irradiation from 20mW He-Ne laser (λ= 632.8 nm). The mice ofsecond group were intratumorally injected with 30 mg HPD/kg of body weight withoutirradiation. Third group mice were received 10 minutes exposure time of He-Ne laserirradiation without HPD injection, while the fourth group was leaved as a control.Photodynamic therapy, which includes HPD and irradiation, has had the most powerful effecton the tumor growth, while the other groups have not showed any significant response. Withmore investigations PDT can be promising anti-breast cancer arsenal

الخلاصةالعلاج الدینامیكي الضوئي للسرطان هو علاج یعتمد على تراكم المحسسات الضوئیة ذات الصلة بالبورفرینات فيخلایا الورم،وتدمیرها لاحقا على أساس التعرض لنوع من الاشعة ذات مواصفات معینة. لقد ظهر إن مشتق الهیماتوبورفرین یتموضع بصورة إنتقائیة في خلایا الانسجة الخبیثة مسبباً تحطیمها بواسطة تولید جذور الأوكسجینالأحادیة وذلك عند تنشیطها بواسطة طول موجي مناسب من الاشعة. إن جذور الاوكسجین الاحادیة المنتجة هيالتي تتسبب في تحطیم الاغشیة والعضیات، مؤدیة الى الموت الخلوي وبالتالي إستئصال الورم. في هذه الدراسة، تمغرس اناث الفئران المختبریة بخلایا الخط الخلوي ام ان 3 المستزرع من سرطانة الغدد اللبنیة للفئران، وقسمت هذهالفئران عشوائیاً الى أربع مجامیع، كل مجموعة تتألف من 10 فئران، فئران المجموعة الاولى حقنت داخل الورمبالمحسس الضوئي المشتق من الهیماتوبورفرین بتركیز 30 ملغم/كغم من وزن الجسم، وتركت في الظلام لمدة 24ساعة، ثم شععت بلیزر هلیوم-نیون ذي قدرة مقدارها 20 ملي واط (الطول الموجي 632,8 نانومتر) بزمن تعریضمقداره 10 دقائق، فئران المجموعة الثانیة حقنت داخل الورم بنفس التركیز من المحسس الضوئي، وتركت في الظلاملمدة 24 ساعة، ولم یتم تشعیعها بلیزر هلیوم- نیون، أما المجموعة الثالثة فتم تعریضها لزمن تشعیع مقداره 10دقائق دون حقنها بالمحسس الضوئي، بینما تركت المجموعة الرابعة كسیطرة دون حقن بالمحسس الضوئي أوتشعیع بلیزر الهلیوم نیون، أظهرت النتائج بأن العلاج الدینامیكي الضوئي الذي تضمن التشعیع اللیزري مقترنابالمحسس الضوئي (مشتق الهیماتوبورفرین) كان الأكثر قوة تثبیطیة لنمو الورم، بینما بقیة المجامیع لم تضهرعلیها أیة فروقات معنویة، ومع المزید من التحریات المستقبلیة ستضاف هذه التقنیة الجدیدة الى ترسانة العلاجاتالمضادة للسرطان خصوصاً سرطان الثدي


Article
Evaluation of Immunohistochemical Staining for Hepatocyte Growth Factor and c-Met in Endometrial Adenocarcinoma

Authors: Lubab Fadhil Talal --- Mohamed Sabaa Challob
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2013 Volume: 12 Issue: 3 Pages: 335-342
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT: BACKGROUND:Endometrial carcinoma is a primary malignant epithelial tumor, usually with glandular differentiation,arising in the endometrium with the potential to invade and metastasize. Hepatocyte growth factor(HGF) and its receptor c- Met have been implicated in uterine development, pregnancy, andendometrial disorders, such as endometriosis and carcinoma. OBJECTIVE:The goal of this study was to evaluate immunohistochemical (IHC) staining patterns of HGF and cMetinendometrialadenocarcinomaandtocorrelatestainingwiththebiologicalbehaviorandoutcomeofendometrialadenocarcinoma.PATIENTSANDMETHOD:Aretrospective study included 45 cases ,who underwent total abdominal hysterectomy and bilateralsalpingo-oopherectomy, between 2005-2010 samples ,were taken from Teaching Laboratories atBaghdad Teaching Hospital/Medical City. Thirty cases were diagnosed as adenocarcinoma of the endometrium, an additional 15 patientsdiagnosed as to have uterine leiomyoma(fibroid) and who had normal endometrium were taken as acontrol group. The patient's age ,tumor grading ,depth of myometrial invasion, presence of pelvic andparaaortic LN metstases, vascular invasion ,and the stage of the disease were noted .The specimens were already fixed in10% formalin , and paraffin embedded. Three sections (4 micron inthickness ) were cut from each paraffin block. One section stained with Haematoxylin and Eosin(H&E) stain , and the other two stained with HGF and c-Met immunostaining antibodies usingpositively charged slides . RESULTS: The mean age of the patients with endometrial adenocarcinoma was 58.5years. There was no statistically significant difference between HGF/c-Met expression and age, tumor grade,stage , myometrial invasion and vascular invasion. There was a statistical significant correlationbetween HGF and c-Met scores with cases showing no pelvic lymph nodes metastases. There is a linear increase in HGF and c-Met expression in both the diseased and control group.Astatistical significant correlation was found in HGF and c-Met scores between the diseased group andthe control group.CONCLUSION:HGF and c-Met staining was significantly different between control group and diseased group. HGF and c-Met Showed Linear increase Expression in both diseased and control group .The c-Met is the high-affinity receptor for hepatocyte growth factor


Article
Immunohistochemical Expression of Her2/Neu Receptor in Human Colorectal Carcinoma (A Clinicopathological Study)

Author: Kifah Hamdan AbdulGhafour
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2014 Volume: 13 Issue: 3 Pages: 424-429
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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ABSTRACT:BACKGROUND: Colorectal adenocarcinoma is the most common type of gastrointestinal cancers in Iraq, and according to the Iraqi cancer registry (ICR) reports, the incidence of colorectal carcinoma was 2.99% of whole body malignancies (ICR 2010). In males, it’s the 5th common cancer while in females it’s the 4th most common cancer. Her2/neu is an important oncogene in breast cancer, but its prevalence and significance in colorectal carcinoma have been documented.OBJECTIVE:To determine the frequency and the pattern of Her2/neu expression in colorectal adenocarcinoma by immunohistochemical technique and to correlate this expression with different clinicopathological parameters.MATERIAL AND METHOD:Twenty five cases of colorectal adenocarcinoma were studied, these cases were diagnosed in private laboratories in Baghdad/Iraq from November 2011 to march 2013. Clinicopathological parameters such as age, gender, pathological diagnosis, including the tumor site, size, lymph nodes status, grade and stage of tumor were taken from patients file. Sections of 4 µm stained by H&E stain and immunohistochemical stained for Her2/neu.Using infiltrative ductal carcinoma of the breast as control positive, evaluation of Her2/neu expression by immunohistochemistry in all cases was performed.RESULTS: Fourteen (56%) of the cases were males, 11 (44%) case were females, with age distribution ranging from (24-89) years, with a mean age of 56.5 years. Tumor size ranges between 2.5-10 cm, with mean of 6.25 cm. Seven (28%) cases were localized in the cecum, 5 (20%) from each rectum, sigmoid and left colon, respectively and 3 (12%) involving more than one segment of the colon. Histologically the tumor grade ranges from moderately differentiated in 23 (92%) cases and poorly differentiated in 2 (8%) cases. Regarding pathological staging (TNM system), 5 (20%) were stage T2, 17 (68%) were stage T3 and 3 (12%) were T4. Lymph node involvement found in 10(40%) of the cases and distant metastasis was found in 2 (12%) cases. Her2/neu expression was present in 4(16%) cases of 25 colorectal adenocarcinoma; there was no correlation with age, sex, histopathological grade, location, lymph nodes status and tumor invasion. CONCLUSION: Concerning data exists about the prevalence of her2/new expression in colorectal adenocarcinoma, there was no significant correlation between her2/neu over expression and tumor size, grade, localization of the primary tumor, lymph nodes status and depth of invasion.


Article
Pathological study of the prostate tumors in Missan governorate

Author: Abbas,Ch. Mraisel
Journal: Misan Journal of Acodemic Studies مجلة ميسان للدراسات الاكاديمية ISSN: 1994697X Year: 2016 Volume: 15 Issue: 29 Pages: 34-48
Publisher: Misan University جامعة ميسان

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Abstract

ABSTRUTProstate cancer is the most frequently diagnosed in males and the second leading cause of cancer-related death in men older than 50 years of age after carcinoma of the lung.The study was performed between (October-2011 to April-2012) the tissue samples were collected of (82) patients aged between( 40- 90 )years was infected with prostate tumors from histopathological laboratory in AL-Sadder hospital in Missan governorate in Iraq .Samples was kept in Buffered Formaldehyde (10%) for period (72) hours after that treated according to histopathological examination and stained with Hematoxylen –Eosin stain The data that were collected from prostate tumors patients was observed high incidence with the ages between (71-80) years represented by 28(34.1%)cases, the ages between (61-70)years represented by 22(26.8 %) cases and the ages between(51-60)years in 18(21.9 %)cases. Histopathological examination of the tissue samples show that the commonest type was adenocarcinmoma represented by (40.2%) ,followed by benign nodular hyperplasia which represented by (34%)cases ,squamousmetaplsia was represented by(15.8%),infract squamous metaplsia represented by(9.7%) of the cases.A higher incidence of prostate cancers was confirmed at ages between (60 - 80)years, the commonest types of prostate tumors that observed in this study were adenocarcinoma and benign nodular hyperplasia, large size of prostate stones may be cause mild to moderate inflammation.


Article
Detection of Human Cytomegalovirus in Colorectal Adenocarcinoma by In Situ Hybridization Technique

Author: Faiza Abdulla Mukhlis
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 / 24108057 Year: 2009 Volume: 51 Issue: 1 Pages: 52-56
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Human Cytomegalovirus (HCMV) infects a wide range of human cells, including colonic epithelial cells that give rise to adenomas and adenocarcinomas. Persistent productive infection of tumor cells is essential for oncomodulation by HCMV.This study aimed to detect HCMV matrix protein using in situ hybridization technique (ISH) in colorectal adenocarcinoma compared to normal colon tissues, and to the presence of cytomegalovirus inclusion bodies in infected colorectal carcinomas.Patients and methods: Twenty six of colorectal adenocarcinomas were obtained in paraffin-blocks compared to 10 normal colon specimens which were age and sex matched as control group. Detection of HCMV was obtained by in situ hybridization technique.Results:The biotinylated probe specific for DNA encoded HCMV-matrix protein showed hybridization with nucleic acid in 20 cases out of (26) of colorectal adenocarcinomas representing (76.9%) compared to normal colon tissue which revealed no hybridization signals. Moderate to high scoring signals were detected in moderate to poorly differentiated groups. Inclusion bodies were detected in 11 (42.3%) cases with positive hybridization signals.Conclusion: HCMV may play a role in the colorectal adenocarcinoma pathogenesis. In situ hybridization test are considered the most sensitive and specific tools for detection of HCMV DNA in tissues. Epidemiological, histopathological identification of cytomegalic inclusion bodies and molecular studies are necessary to confirm the association of HCMV and colorectal tumorogenesis in Iraqi population.

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