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EVALUATION OF SEMINAL FLUID PARAMETERS AFTER IN VITRO SPERM PREPARATION TECHNIQUE IN NON VARICOCELIC AND VARICOCELIC INFERTILE MEN UNDERGOING VARICOCELECTOMY

Authors: Basim Khamees Gootee باسم خميس --- Diyaa Abed Auda ضياء عبد عودة
Journal: Thi-Qar Medical Journal مجلة ذي قار الطبية ISSN: 19929218 Year: 2012 Volume: 6 Issue: 1 Pages: 151-162
Publisher: Thi-Qar University جامعة ذي قار

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Abstract

This study was designed to evaluate and compare the results of semen parameters, sperm membrane potential integrity and viability for non varicocelic and varicocelic infertile men after varicocelectomy. Thirty semen samples (varicocelic) and twenty normozoospermic men (non varicocelic) as a control group were collected by masturbation in the special semen room collection and prepared by simple layering technique. The semen samples were analyzed and prepared by standard semen parameters. Furthermore, sperm concentration, sperm motility, progressive sperm motility, sperm agglutination, normal sperm morphology, sperm HOST, and sperm Eosin stain were evaluated according to standard WHO criteria (1999). However, direct immunobead assay was used to determine the presence of AS-AB bound on sperm surface. For preparation technique, sperm prepared and incubated for 30 minute in 5% CO2 at 37ºC after in vitro sperm processing. The results of the present study indicate a highly significant (P<0.001) differences in all sperm functions parameters of varicocelic infertile men in AS-ABs positive (HOST and Eosin stain; negative) as compare with that noticed in normozoospermic men in AS-AB negative (HOST and Eosin stain; positive). It was concluded that there are a strong positive correlation between varicocele and antisperm antibodies generation and between antisperm antibodies and semen characteristics and HOST-Vitality test. Further studies are recommended to assess the detrimental effect of AS-ABs on DNA damage and embryo quality after in vitro fertilization and embryo transfer in assisted reproductive technologies (IVF-ET-ART).


Article
DETERMINATION SOME OF COMPLEMENT COMPONENTS IN INFERTILITY WOMEN WITH ANTISPERM ANTIBODIES
تحديد مستوى بعض مكونات المتمم في النساء اللواتي يعانين من العقم

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Abstract

Background: Classical activation of complement by antigen and antibody complex leads to formation of membrane attack complex (MAC) that leads to formation holes on the spermatozoa ending in their destruction. Objective: To determine the complements levels and antisperm antibodies in the sera of infertile women of unknown etiology.Patients and methods: Study group consisted of 45 infertile women consulting Kammal El-Sammarei Hospital for Infertility and In Vitro Fertilization from Jun -2008 to June-2009. Twenty-four (53.3%) patients had primary infertility and the rest had secondary infertility. Control group: consisted of thirty fertile women. Blood samples were collected from them and anti sperm antibodies in the serum were detected by indirect immunofluorescence test (EURO IMMUNE –GERMENY). In addition to that serum were tested for complement levels (C3 and C4) using single radial immune diffusions test (BINDARID) KIT BIRMINGHAM .UK.Results: Detections of antisperm antibodies in the serum of infertile women were (64.4%) which is significantly (p<0.05) higher from control group using indirect immunofluorescence test. There was a significant (p=0.000) difference in the complements levels among infertile women who had ASA positive and ASA negative and control group.Conclusions: These higher levels of complement components may be due to activation of classical pathway by ASA that directed against sperm antigens ending in defect in function and motility of the sperms.Key words: Infertility, antisperm antibody, complement.

خلفية الدراسة: المتمم هو عبارة عن سلسلة من الانزيمات البروتينية, الذى يشكل الجزء الاساسي للجهاز المناعي. تحفيز هذا الجهاز يؤدي في النهاية الى تكوين ثقوب على جدار الخلية.هدف الدراسة: تحديد مستوى المتمم في مصل النساء اللواتي يعانين من العقم مع تواجد الاجسام المناعية الذاتية للحيامن .طريقة العمل: تتالف الدراسة من 45 مريضة يعانين من العقم ويراجعن مستشفى كمال السامرائى للعقم للفترة من حزيران-2008 الى حزيران 2009 ومجموعة اخرى تتألف من 30 امرأة من النساء الطبيعيات القادرات على الانجاب بصورة طبيعية. تم سحب الدم من هاتين الفئتين وتم تحديد مستوى المتمم C3,C4 وكذلك تحديد الاجسام المناعية الذاتية الموجهة ضد الحيامن.النتائـج: كان هناك فرق مهم احصائيا بين تلك الفئتين بالنسبة للاجسام المناعية الذاتية للحيامن والمتمم C3,C4.الاستنتاج: احتمال ارتفاع نسبة المتمم نتيجة لارتفاع نسبة تواجد الاجسام المناعية الذاتية للحيامن ممايودي الى خلل في عمل الحيامن. مفتاح الكلمات: عقم, الاجسام المناعية الذاتية للحيامن, المتمم


Article
Relationship between potency of hypo osmotic swelling test and antisperm antibody assay for fertile and infertile men in Thi-Qar governorate
العلاقة بين فعالية فحص كفاءة النطفة البشرية تحت الضغط الازموزي الواطئ مع فحص الضداد المناعية المضادة للنطف للرجال الاسويا والعقيمين في محافظة ذي قار

Author: باسم خميس كوتي الركابي
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2013 Volume: 3 Issue: 5 Pages: 44-48
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background;The sperm plasma membrane activity and absence of antisperm antibodies in infertile patients ejaculate significantly associated with fertilization potential of human spermatozoa. The sperm viability detected by hypoosmotic swelling test (HOST) and autoimmune infertility detected by antisperm antibody assay presented diagnostic and prognostic tools for male infertility factors.Objective:This study was devised to compare the results of hypoosmotic swelling test and antisperm antibody assay for normozoospermic men and infertile patients affected by autoimmune infertilityMethods:Thirty semen samples were collected by masturbation and prepared by direct layering technique. Hypo-osmotic swelling test was performed by mixing 0.1 ml of semen with 0.9 ml of a 150 mOsmI L NaCI as a hypo-osmotic solution and direct immunobead assay were used to determine the presence of Antisperm Antibodies (ASA) bound on sperm surface. However, sperm concentration, sperm motility, and normal sperm morphology were evaluated according to World Health Organization (WHO) criteria and subjected to HOS test and ASA assay. The sperm prepared and incubated for 30 minute in 5% C02 at 37°C after in vitro sperm preparation.Result*:The results of the present study reported that the percentage of HOS test score in antisperm antibodies positive sperm samples (52.30 ± 5.2) was significantly lower than that noticed in antisperm antibodies negative (70.30 ± 3.4) sperm samples.Conclusion:It was concluded that the use of HOS test as a simple and diagnostic and prognostic tools for identify male infertility. Further studies are suggested to assess the effect of ASA on sperm plasma functional integrity after ICSI and in vitro fertilization and embryo transfers (IVF-ET).

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