research centers


Search results: Found 8

Listing 1 - 8 of 8
Sort by

Article
THE PREVALENCE AND CLINICAL SIGNIFICANCE OF PERINUCLEAR ANTINEUTROPHIL CYTOPLASMIC ANTIBODY IN PATIENTS WITH INDETERMINATE COLITIS

Loading...
Loading...
Abstract

Background: Indeterminate colitis (IC), a term originated by pathologists to characterize confounding histopathlogic appearance of resected mucosa, has become catch phrase for cases in which diagnostic criteria at all levels elude classification as Crohn's disease (CD) or ulcerative colitis (UC).OBJECTIVES: evaluate the prevalence of pANCA expression in the sera and its isotypes. Patients and methods: PATIENTS GROUP consisted of 60 patients (40 males and 20 females) with indeterminate colitis and their age range was (19-84 years). CONTROL GROUP consisted of 30 (15 males and 15 females) healthy volunteers and their age range was (20- 66 years). Antineutrophil cytoplasmic ( pANCA and cANCA) testing was performed by an IIF technique on ethanol fixed human EOH granulocytes as substrate (EUROIMMUNE- Germany). Sigmoidoscope and colonoscope examination were done for the patients group and biopsies were taken from the patients for histopathological examination.Results:Serological results of ANCA showed a significant increased frequency of pANCA (63.3%) in indeterminate colitis patients as compared to controls (p=0.000). The highest percentage of this pANCA titer was 1:10 (p=0.000) then 1:100 (p=0.008) and most of them was IgG (53.3%) (p=0.000). Sensitivity of pANCA was 60%, specificity of pANCA was 40%, positive predictive value of pANCA was 61.1% and negative predictive value of pANCA was 66.6%. cANCA did not demonstrated in both groups. Conclusions : pANCA was more prevalent in indeterminate colitis and could be used as a predictive serological marker for the outcome of disease.


Article
Detection of Human Leukocyte Antigen and Celiac Disease Auto Antibodies in serum of Patients with Multiple Sclerosis
التحري عن مستضدات التوافق النسيجي والاضداد الذاتية لحساسية الحنطة في مصول المرضى المصابين بتصلب الاعصاب المتعدد

Authors: Rana S. Aboud رنا سعدي عبود --- May K. Ismael مي خليل اسماعيل --- Haider J.Mohammed حيدر جاسم محمد
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 4A Pages: 1477-1483
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

To determine the important pathogenic role of celiac disease in triggering several autoimmune disease, thirty patients with Multiple Sclerosis of ages (22-55) years have been investigated and compared with 25 healthy individuals. All the studied groups were carried out to measure anti-tissue transglutaminase antibodies IgA IgG by ELISA test, anti-reticulin antibodies IgA and IgG, and anti-endomysial antibodies IgA and IgG by IFAT. There was a significant elevation in the concentration of anti-tissue transglutaminase antibodies IgA and IgG compared to control groups (P≤0.05), there was 4(13.33%) positive results for anti-reticulin antibodies IgA and IgG , 3(10%) positive results for anti-endomysial antibodies IgA and IgG . There were 4 positive results (13.33%) for HLA-DQ8 by using HLA-DQ8 Real-Time PCR test. These results indicated that patients with celiac disease play an important role in pathogenesis of Multiple Sclerosis.

غرض تعيين الدور الامراضي المهم لمرض حساسية الحنطة في قدح العديد من امراض المناعة الذاتية، تم التحري عن (30) شخص مريض يعانون من تصلب الاعصاب المتعدد باعمار تتراوح من (22-55) سنة وتمت المقارنة مع (25) شخص سليم . خضعت جميع عينات الدراسة لقياس مستوى اضداد anti- tissue transglutaminaseAb الصنف ( (IgA,IgG باستخدام تقنية الامتزاز المناعي المرتبط بالانظيم ،وقياس مستوى اضداد reticulinAb-anti الصنف ( IgA,IgG) و anti-endomysial Ab (IgA,IgG) باستخدام تقنية التالق المناعي غيرالمباشر. اظهرت نتائج الدراسة ارتفاعا معنويا0.05) P≤)في تركيزاضداد anti-tissue transglutaminaseAb الصنف (IgA,IgG) مقارنة بمجاميع السيطرة ،كمالوحظ ان هنالك اربعة نتائج موجبة (13.33% ) بالنسبة لاضداد ( IgA,IgG) reticulinAb- antiوثلاثة نتائج موجبة10% بالنسبةلاضداد anti-endomysialAb (IgA, IgG) ، كما أظهرت نتائج الدراسة ان هنالك اربعة نتائج موجبة(13.33%) بالنسبة للHLA-DQ8 باستخدام تقنيةHLA-DQ8 Real time PCR . تشير نتائج الدراسة الحالية بان مرض حساسية الحنطة يلعب دورا مهما في قدح مرض تصلب الاعصاب المتعدد.


Article
Human Leukocyte Antigens class II influence the expression of Glutamic Acid Decarboxylase auto antibodies in Type Diabetic children and their Siblings

Author: Eman Mahdi Saleh ايمان مهدي صالح
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2010 Volume: 6 Issue: 1 Pages: 52-61
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

Background: The immunogenetic predisposition may be considered as an important factor for the development of Type 1 Diabetes Mellitus (T1DM) in association with the HLA antigens. Objective:This study was designed to investigate the role of HLA-class II antigens in the etiology of type T1DM and in prediction of this disease in siblings, and its effect on expression of glutamic acid decarboxylase autoantibodies (GADA). methods:Sixty children who were newly diagnosed type 1 diabetes (diagnosed less than five months) were selected. Their age ranged from 3-17 years. Another 50 healthy siblings were available for this study, their ages range from 3-16 years. Eighty apparently healthy control subjects, matched with age (4-17) years, sex and ethnic backgrounds (Iraqi Arabs) underwent the HLA-typing examination. Finally 50 healthy individuals were selected randomly to undergo GADA test.Results:At HLA-class II region, DR3 and DR4 were significantly increased in patients (53.33 vs.26.25% and 50.0 vs. 12.5% respectively) as compared to controls. In addition to that, T1DM was significantly associatedwith DQ2 (33.33 vs.15%) and DQ3 (40.0 vs.20%) antigens as compared to controls, suggesting that these antigens had a role in disease susceptibility, while the frequency of DR2 and DQ1 antigens were significantly lowered in patients compared to controls (6.66 vs.25% and 6.66 vs.22.5% respectively). These molecules might have protective effect. In siblings a significant increase frequency of DR4 antigen (34.0 vs.12.5%) was observed in comparison to controls, suggesting that it might be much useful for predicting T1DM in affected families.Anti-GAD autoantibodies were present in 50% of Type 1Diabetic children, and in 16% of their siblings. High proportion of GADA was found in the patients carrying HLA-DR3/DR4 heterozygous.conclusion:Both the T1DM patients and their siblings shared the HLA- DQ1 as protective antigens, while DR3 and DR4 were susceptible one, and high proportion of GADA was found in the T1DM patients and siblings carrying HLA-DR3/DR4 heterozygous.


Article
Antithyroid Autoantibody in Unexplained Recurrent Abortion

Authors: Maha M. AL-Bayati --- Asmaa Mohammed Abid --- Haider Fa’ak Abd-EL-Kareem --- Aseel Salim Mousa
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2012 Volume: 11 Issue: 3 Pages: 308-314
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

Loading...
Loading...
Abstract

ABSTRACT:BACKGROUND:Recurrent miscarriage is a common complication of pregnancy and when the cause is unknown; relationship of autoimmune thyroid disease to pregnancy loss has been the object of considerable interest.OBJECTIVE:To estimate whether thyroid auto-antibodies (thyroglobulin and thyroid peroxidase antibodies) could be used as a marker for detection of abortion in cases with unexplained recurrent miscarriage.METHODS:This study was carried out at the department of obstetrics and gynecology in AL- Kadhymia Teaching Hospital from March 2008 through September 2009. Fifty pregnant women in their first trimester (7th – 12th weeks) were followed up till 20th weeks of gestation by the researcher himself (they have three previous unexplained first and second trimester miscarriages).Thyroid peroxidase (TPOAB) measurement by ELISA and thyroglobulin antibodies (TGAB) by slide methods with flourescein were done to the patients, cases with abortions were reported and the status of thyroid antibodies was evaluated. Free T3; free T4, TSH measured by RIA technique.RESULTS:In eighteen patients the antibody titer was positive while thirty-two had negative tests for thyroid auto-antibodies. TGABS (thirty-six) were negative, (thirty-three) continue pregnancy and only (three) aborted. TGABS (fourteen) were positive, (four) cases continue and (ten) aborted. TPOABS (forty-one) were negative (thirty-nine) cases continue pregnancy and only (two) aborted. TPOABS (nine) cases were positive, (seven aborted and only (two) cases continue pregnancy. This study showed a significant association between AB. Positivity and maternal age.CONCLUSION:Thyroid auto antibodies can serve as a useful biochemical marker for cases with unexplained recurrent miscarriage and there is a positive relation with increasing maternal age (thyroid auto antibodies).


Article
The Correlation between the levels of Coxsackie B viruses Ig's and the glutamic decarboxylase auto antibodies in diabetesmellitus type 1 patients

Author: Raghad Al-Suhail
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2012 Volume: 11 Issue: 2 Pages: 49-52
Publisher: Al-Mustansyriah University الجامعة المستنصرية

Loading...
Loading...
Abstract

About 44% of diabetes mellitus type 1 (DM1) patients were positive forone or more Coxsackie B virus IgM Serotypes, compared to zero in thehealthy control subjects, those samples showed a significant strongpositive correlation with glutamic acid decarboxylase auto-antibodies,this may indicate the role of these viruses in the pathogenesis of DMI.This work was done in cooperation between Baghdad University,Baghdad – Iraq and the National Center of diabetes, endocrinology andgenetic, Amman – Jordan, in a sabbatical leave study.


Article
Detection of some auto antibodies of Celiac disease in Sera of patients with Chronic Hepatitis B virus
التحري عن بعض الأضداد الذاتية لداء الزلاقي في مصول المرضى المصابين بالتهاب الكبد الفيروسي المزمن النمط - ب-

Authors: Shiamaa.G.Abid شيماء غني عبد --- Rana.S.Aboud رنا سعدي عبود --- Safa.A.Abudl-Razak صفاء عبد الكريم عبد الرزاق
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 1B Pages: 379-384
Publisher: Baghdad University جامعة بغداد

Loading...
Loading...
Abstract

To determine the relationship between chronic hepatitis B virus and autoimmune celiac disease, seventy five patients with chronic hepatitis B virus of ages (8-70) years have been investigated and compared to 50 healthy individuals. All the studied groups were carried out to measure anti-tissue transglutaminase antibodies IgA and IgG by ELISA test and anti-endomysial antibodies IgA and IgG by IIFT. There was a significant elevation in the concentration of anti-tissue transglutaminase antibodies IgA and IgG compared to control groups (P< 0.01) .The prevalence of antiTtG IgA and IgG was 14.67% and 12.0% respectively.There was a highly significant difference (P<0.01) when compared between studied groups .While the prevalence of anti-endomysial antibodies IgA and IgG was 9.33% and 4.0% respectively. There was a highly significant difference(P< 0.01) when compared between studied groups. These results indicated that infection with chronic hepatitis B virus may play an important role in pathogenesis of celiac disease.

لغرض تعيين العلاقه بين التهاب الكبد الفيروسي المزمن النمط - ب- والداءالزلاقي الذاتي المناعة , تم التحري عن (75) شخص مصاب بالتهاب الكبد الفيروسي المزمن النمط - ب- باعمار تتراوح من (8-70) سنة وتمت المقارنة مع (50) شخص سليم . خضعت جميع عينات الدراسة لقياس مستوى اضدادAbs anti-tissuetransglutaminase الصنف (IgA,IgG)باستخدام تقنية الامتزازالمناعي المرتبط بالانظيم و anti-endomysial Abs (IgA,IgG) باستخدام تقنية التالق المناعي غيرالمباشر. اظهرت نتائج الدراسة ارتفاعا معنويا(P< 0.01) في تركيزاضداد anti-tissue transglutaminase Abs الصنف (IgA,IgG) مقارنة بمجاميع السيطرة.كانت نسب اضداد anti-tissuetransglutaminase Absالصنف ( 14.70% , (9/75) 12.0% ( gA, IgG (11/75)على التوالي وقد كانت هنالك فروق معنوية عاليه (P < 0.01) بين مجاميع الدراسة بينما كانت نسب اضداد (7/75) 9.33% anti-endomysial Abs (IgA,IgG), %0.(3/75)4 على التوالي وهنالك فروق معنوية عاليه (P< 0.01) بين مجاميع الدراسة.تشير الدراسة بان الخمج بالتهاب الكبد الفيروسي المزمن النمط - ب- يمكن أن يلعب دورا مهما في أمراضية حساسية الحنطة.


Article
Detection of anti-Myalin and anti-axonal auto-antibodies in Iraqi patients with multiple sclerosis
دراسة الاجسام المضادة الذاتية للمايلين والمحاور العصبية لمرضى تصلب الاعصاب المتعدد في العراق

Author: Rasha Majid Abdulamir Al-hemiary رشا ماجد عبد الامير الحميري
Journal: Jornal of Biotechnology Research Center مجلة مركز بحوث التقنيات الاحيائية ISSN: 18151140 Year: 2013 Volume: 7 Issue: 1 Pages: 21-28
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Multiple Sclerosis (MS) is characterized by demyelination of different parts of the central nervous system (CNS) leaving scars (sclerosis) which leaves the neuron and the axis highly attenuated. It is one of the most important inflammatory diseases of the CNS that causes deficits with the progression of neurological disease. This study included 65 MS patients (37 relapsing-remitting (RR), 21 secondary-progressive (SP), 7 primary-progressive (PP) and 50 apparently healthy individuals controls. The results showed low level of auto-antibodies to myelin associated glycoprotein (anti-MAG) in MS patients, while anti-axon antibodies had high level. This means that the production of these auto-antibodies in different stages of the disease increase its effect in the diagnosis and the possibility that it had some role in the progress and development of MS, so the use of anti axonal antibodies test in the diagnosis is an important marker.

مرض تصلب الأعصاب المتعدد (MS) يمتاز بفقدان الغمد النخاعيني في مناطق مختلفة من الجهاز العصبي تاركا ندب ، مما يجعل العصبة والمحور العصبي هزيلان وهو من أكثر واهم أمراض التهاب الجهاز العصبي المركزي التي تسبب انتكاسات مع تقدم في العجز العصبي . شملت الدراسة 65عينة مرضــية ، ( PPMS 7,SPMS 21, RRMS 37) تـعاني من مرض (MS) ، كما شملت ايــضا 50 عيــنة ســيـطرة مكونة من افراد اصحاء ظاهرياً . نتائج البحث اظهرت وجود مستويات قليلة من الاجسام المضادة الذاتية للمايلين ((MAG في مصول مرضى الـ MS بينما اظهرت الاضداد الذاتية للمحاور العصبية ( (Anti-axon مستويات عالية في مرضى الـ MS مما يعني انتاج هذه الاضداد في مراحل مختلفة للمرض وزيادة تاثيرها في التشخيص العصبي للمرضى واحتمال لعبها دورا مهما في تقدم شدة المرض وتطوره ، وبالتالي يقترح استخدام اختبار الاضداد الذاتية للمحاور العصبية anti axonal) ) في تشخيص مرض MS .


Article
DETECTION OF AUTO-ANTIBODIES SPECIFIC TO ISLETS CELLS INPATIENTSWITH THYROID DYSFUNCTION BY USING BIOCHIPSTECHNIQUE

Author: Haider J.Muhammad
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2009 Volume: 12 Issue: 3 Pages: 133-138
Publisher: Al-Nahrain University جامعة النهرين

Loading...
Loading...
Abstract

Abstract
The aim of this study was to detect the auto immune Type 1 diabetes among the disorders with
thyroid dysfunction (hyperthyroidism and hypothyroidism).The study included 50 patients suffering
from thyroid dysfunction, their aged was from (1-30) years. They were selected randomly from the
Al-kindy center for infertility and indocrinology. The control group consisted of (20) healthy
subjects comparable for age and sex of study groups. Thyroid function tests (thyroid-stimulating
hormone [TSH], thyroxin, and triiodothyronine) measured in by using miniVIDAS system and
auto-antibodies for Beta cells in pancreas estimated in the same patients by using
immunofluorescent method (IFAT), the method named BIOCHIPS Mosaic. Results of thyroid
function test revealed that 25 patients were evaluated to have hyperthyroidism and 25 patients with
hypothyroidism and the results of BIOCHIPS showed hyperthyroidism group was having autoantibodies
specific to islets cells with 8 percent of all the subjects (two from 25 patients) .While
there was no signs of auto antibodies for islets cells in hypothyroidism group. The study revealed
that the prevalence of thyroid dysfunction was higher in women than men.

الخلاصة إن الهدف من الدراسة هو التحري عن المرض المناعي الذاتي لداء السكر من النوع الأول في المرضى المصابين باعتلال الغدة الدرقية (المفرط والمنقوص). تضمنت الدراسة (50) مريضا يعانون من اعتلال الغدة الدرقية, تتراوح أعمارهم من (1-30) سنة. تم اختيار المرضى بصورة عشوائية من مركز الكندي للغدد الصم والعقم. و تضمنت الدراسة مجموعة من السيطرة (20) شخص من الأصحاء وتمت المقارنة مع العمر والجنس مع بقية مجاميع الدراسة. تضمنت اختبارات الغدة الدرقية (الهرمون المحفز للغدة الدرقية TSH ,وهرمون الثايروكسينT4 وهورهمون الثايرونين T3) ,تم قياسها باستخدام جهاز المنيفايدز وتم قياس الأضداد الذاتية لخلايا بيتا في غدة البنكرياس في نفس المرضى باستعمال تقنية التفلور المناعي غير المباشر بطريقة تدعى تبرقش البايوجبس. أظهرت نتائج وظائف الغدة الدرقية أن (25) مريض مصاب بالغدة الدرقية من النوع المفرط و(25) مريض مصاب بالغدة الدرقية من النوع المنقوص.وكشفت نتائج طريقة البايوجبس أن مجموعة الدراسة للمرضى المصابين بالغدة الدرقية المفرط يمتلكون أضداد ذاتية نوعية لخلايا بيتا (خلايا الجزر) بنسبة 8% (2 من 25) مريض بينما لا توجد علامات عن هذه الأضداد الذاتية لخلايا الجزر في مجموعة الدراسة لمرضى الغدة الدرقية المنقوص. وقد كشفت الدراسة بانتشار اعتلالات الغدة الدرقية في النساء أكثر منه في الرجال.

Listing 1 - 8 of 8
Sort by
Narrow your search

Resource type

article (8)


Language

English (8)


Year
From To Submit

2015 (1)

2014 (1)

2013 (1)

2012 (2)

2011 (1)

More...