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Article
Mitotic activity of cultured human lymphocytes treated with gold nanoparticles

Authors: Sumaiah I. Hussein --- Yahya D. Saihood --- Ayda M. Majeed --- Heba F. Hassan --- et al.
Journal: Iraqi Journal of Cancer and Medical Genetics المجلة العراقية للسرطان والوراثة الطبية ISSN: 20786123 Year: 2017 Volume: 10 Issue: 2 Pages: 212-215
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Potential health and environmental effects of nanoparticles need to be thoroughly assessed before their widespread commercialization.The present investigation was planned with the aims to determine the effects of gold nanoparticles (GNPs) onblast (BI) and mitotic (MI) indices of cultured lymphocytes. The results revealed that BI (50.3 ± 2.3, 30.2 ± 1.9, 10.5 ± 0.7 and0.0%, respectively) and MI (70.1 ± 2.9, 20.4 ± 1.1, 5.3 ± 0.1 and 0.0%, respectively) showed a gradual decreased percentageas the concentration of GNPs was increased from 0.085 to 0.66 μg/mL, and the difference was significant compared to controlculture (81.6 ± 2.5 and 90.2 ± 3.7%, respectively). A maximum inhibition of BI and MI was occurred at the concentration 0.66μg/mL. In conclusion, GNPs can be considered as a growth inhibiting agent, but it is too early to reach a final conclusion inthis regard and further investigations are required to elucidate the biochemical mechanisms involved in inhibiting the mitoticactivity of lymphocytes.


Article
The Cytogenetic and pathological effects of toluene on female Mice

Authors: Bushra I. AL-Kaisie --- Hussein A. Al-Hillali
Journal: Jornal of Al-Muthanna for Agricultural Sciences مجلة المثنى للعلوم الزراعية ISSN: 40862226 Year: 2017 Volume: 5 Issue: 2 Pages: 56-64
Publisher: Al-Muthanna University جامعة المثنى

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Abstract

The study objective was to explore the effects of toluene on cytogenetic status incluin mitotic index, blast index, chromosomal aberration and DNA damaged, one hundred eighty adult female albino mice. They were selected randomly, equally, and then allocated into three bunches each was divided into three groups to represent control group, group 1 (G1) and group 2 (G2) administration from (LD50). These were administrated orally and continuously for 60 days with toluene at doses of 0.1 ml / kg B.W. ,and 0,2 ml / Kg B.W. Control group received olive oil and distilled water, G1 oral administration from LD50 daily for 30 days and G2 oral administration from LD50 daily for 60 days. Fasting blood samples collected by cardiac puncture technique at 0, 30 and 60 days of experimental. Blood drawn by cardiac puncture technique for measuring some parameters related to toluene toxicity on reproductive organ and cytogenetic status at 60 days. The occurrence of toxic effects of toluene manifested by central nervous sings including abnormal gait of animals in cage, tremor, ataxia and convulsion, rapid and shallow respiration, irritation of eye, as well as structural pathological changes in uterus and ovaries tissues sections including enlargement of ovaries with adhesion edematous, sloughing of endometrium, pyometria, necrosis, degeneration of ovaries cells with lymphocytic (infiltration and coughing) around blood vessels and lymphocytic granuloma with peritonitis , the results also showed toxicity effects of toluene on mitotic index , blast index , significant increase in chromosomal aberration mostly fragment and DNA damaged represented by (tail length ,tail intensity and tail movement).

صممت هذه الدراسه لمعرفه الدور السمي للتلوين في اناث الفئران البالغه على الجهاز التناسلي الانثوي ( المبايض والرحم ) والتغيرات الخلوية الوراثية مع دراسه التغيرات المرضيه النسيجيه ( الرحم والمبيض) . تم استخدام ( 120 ) من اناث الفئران البيض البالغه وقسمت عشوائيا وبالتساوي الى ثلاث مجاميع : مجموعه السيطره , المجموعه 1 ( G1 ) , المجموعه 2 (2(Gتجرع من الجرعه النصف قاتله ( LD50 ) جرعت المجموعتين يوميا ولمدة 60 يوميا عن طريق الفم من مادة التلوين وبتركيز (0.2 مل / كلغم من وزن الجسم ) و كالتالي : مجموعه السيطرة جرعت عن طريق الفم وبزيت الزيتون والماء المقطر فقط , في حين جرعت المجموعه 1 ( G1 ) عن طريق الفم لمدة 30 يوما , المجموعه 2( ( G2عن طريق الفم لمدة 60 يوما . جمعت عينات الدم في الايام 0 , 30 , 60 عن طريق تقنيه ثقب القلب لغرض اجراء المعاير التعلقه بسميه التلوين على الجهاز التناسلي الانثوي والتي تشمل الانحرافات الكرومسوميه ( اختبار دالة الانقسام mitotic index , اختبار النوى الصغرى Micronuclei assay , تقدير المذنب ( الهالة ) comet assay ) والتغييرات المرضية للجهاز التناسلي الانثوي. اظهرت نتائج الدراسه الحاليه التغيرات المرضيه العيانيه والمجهريه للرحم والمبيض والتي اهمهاالتضخم مع التصاقات وانسلاخ بطانه الرحم , والرحم القيحي والنخرمع ارتشاح بالخلايا اللمفيه في النسيج والتكفف حول الاوعيه الدمويه , والورم الحبيبي اللمفي والتهاب البيرتون , كما اظهرت الدراسه ارتفاع معنوي في الانحرافات الكروموسوميه معظمها التجزء وتقنيه تقدير المذنب ( الهالة ) . (طول وكثافه وحركة الذيل )

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