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Article
New Methods for Establishment of Lymphoblastoid Cell Lines

Author: Ismail Ibrahim Latif
Journal: Diyala Journal For Pure Science مجلة ديالى للعلوم الصرفة ISSN: 83732222 25189255 Year: 2010 Volume: 6 Issue: 3 Pages: 277-287
Publisher: Diyala University جامعة ديالى

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Abstract

Background: Human lymphocytes can be isolated from whole blood by centrifugation using a commercially available high-density medium. This allows a single step gradient separation of blood, which yields the mononuclear cell ,the lymphocyte can be cultured specifically and will outgrow the others, eventually resulting in highly enriched population.Objective: this series of experiments were done as a trial to establish a new method for preparation of lymphocytes cell line.Methods: the same protocols were applied to all individuals, they were separated in to two groups:- Group 1:- one ml of heparinized blood were cultured in tissue culture flask containing 9 ml of stimulation medium and incubated in tissue culture flasks at 37ºC (4) . The flasks were subcultured at 37 OC in CO2 incubator every 3 days.Group 2:- Mononuclear cells were separated from whole blood, washed, counted, assessed for viability, then 1 ml of 2x106 cells were cultured in tissue culture flask containing stimulation medium and. The flask was incubated at 37 OC in CO2 incubator for 3 days. Another 1ml of mononuclear cells was cultured in tissue culture flask containing 1ml of growth medium, the flasks were incubated at 37 OC in CO2 incubator for 3 days.For both groups the cell cultures were propagated and maintained. Results: After initiation of cell culture for both groups, the mononuclear cell cultures were maintained up for 3 weeks, pure rich mononuclear cells were obtained and seen under inverted microscope.Conclusion: a new method for establishment of lymphoblast cell line were developed by cultivation of lymphocyte from whole blood . pure rich lymphocytes culture were obtained and maintained


Article
Detection of Respiratory Syncytial Virus infection in Clinical Samples Using Immunofluorescence Test
الكشف عن الأصابة بفيروس Respiratory Syncytial Virus في العينات السريرية بواسطة اختبار التألق المناعي

Authors: Raghad G. Al-Suhail رغد غالب السهيل --- Layla F. Ali1 ليلى فؤاد علي --- Iman M .Aufi ايمان مطشر عوفي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 2C Pages: 1612-1616
Publisher: Baghdad University جامعة بغداد

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Abstract

Current study aimed to investigate the Respiratory Syncytial Virus (RSV) in nasal secretion and throat swab samples of hospitalized patients with symptoms of respiratory tract infection using Immunofluorescence test. Previously these samples were tested for Respiratory Syncytial Virus infection by reverse transcriptase-PCR assay. The positive samples were tested by Immunofluorescence assay in monolayer confluent of Hep 2 cell line. The results showed that the positive samples using the RT-PCR test were positive in this test. These results reveal that Immunofluorescence test is sensitive method for detection the infection with RSV.

هدفت الدراسة الحالية للتحري عن الاصابة بفيروسRespiratory Syncytial Virus (RSV) في عينات من إفرازات الأنف و الحلق من المرضى الراقدين في المستشفى والذين يعانون من أعراض عدوى الجهاز التنفسي بواسطة اختبار التألق المناعي. في البداية تم اختبار هذه العينات لعدوى فيروس بواسطةreverse transcriptase - PCR .العينات الموجبة تم فحصها باختبار التألق المناعي في خطوط خلاياHep 2 . أظهرت النتائج ان العينات الموجبة بفحص RT-PCR كانت موجبة باختبار التألق المناعي. هذه النتائج تبين ان اختبار التألق المناعي ذو حساسية عالية لتشخيص الاصابة بفيروس RSV.


Article
Evaluation of clinically suspected pertussis in children attending Rapareen Teaching Hospital in Erbil Governorate
تقييم السعال الديكي المشتبه به سريريًا عند الأطفال الملتحقين بمستشفى رابرين التعليمي في محافظة أربيل

Authors: Shireen A. Dzayee --- Nazdar E. Alkhateeb --- Dlair A. Chlalabi
Journal: Zanco Journal of Medical Sciences مجلة زانكو للعلوم الطبية ISSN: 19955588/19955596 Year: 2015 Volume: 19 Issue: 3 Pages: 1051-1056
Publisher: Hawler Medical Univeristy جامعة هولير الطبية

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Abstract

Background and objective: Pertussis is one of the vaccine preventable diseases. Bordetella pertussis (B. Pertussis) continues to circulate even in countries with good childhood vaccination. The aim of this study was to evaluate patients suspected or confirmed to have pertussis in relation to certain clinical and laboratory data. Methods: A prospective study was conducted in Rapareen Teaching Hospital from June 1st, to September 1st, 2010. The study enrolled 651 inpatients below five years of age with respiratory tract infection with 72 of them having initial suspicion of pertussis. Demographic and clinical data were obtained from them and nasopharyngeal swab were sent for culture for isolation of microorganisms as well as blood samples obtained for haematological assessment (white blood cell differential count and platelet count). Those confirmed to have pertussis and para-pertussis were considered as group A while other culture results were considered group B. Results: Most of patients were below one year of age (56.9%) with large numbers were not properly vaccinated and they had positive family history of paroxysmal cough (68.1% and 58.3%, respectively). Twenty one patients confirmed to have pertussis and parapertussis by culture methods. There was no significant difference between patients with group A when compared to group B regarding gender, vaccination status, history of post-tussive vomiting or whoop and family history of chronic cough. Mean platelet count was significantly different between two groups, with pertussis and parap-pertussis cases had less platelet count.Conclusion: Near half of patients with B. pertussis and parapertussis have a defect in their vaccination program.

Keywords

Pertussis --- Culture --- Cough --- Patients


Article
Assessment of transient bacteremia following various oral and dental interventions

Authors: Mohammad Kh Hasouni --- Wael T Al–Wattar --- Mahmoud YM Taha
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2003 Volume: 3 Issue: 1 Pages: 50-56
Publisher: Mosul University جامعة الموصل

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Abstract

The incidence of bacteremia following various dental interventions of 280 selected patients were studied using blood culture technique with aerobic and anaerobic cultivation. The incidence of bacteremia after multiple teeth extraction was higher (51.72%) than those single tooth extraction (44%). The incidence of bacteremia after local anaesthetic injection differs according to the type of injections significantly. In scaling and root planning the use of local prophylactic solution reduced the incidence of bacteremia from 37.7% to 13.9%. Poor oral hygiene had an effect on incidence of bacteremia at different dental interventions. The role of sex and age in bacteremia was variable. Aerobic bacteria were more than anaerobic and the most common bacteria isolated were Streptococcus viridans (30.5%) and Staphylococcus aureus (19.7%).


Article
Culture and Critical Thinking Skills for Language Learners

Author: Lec.Dr. Muna Mohammed Abbas Alkhateeb
Journal: AL-AMEED JOURNAL مجلة العميد ISSN: 22270345 23119152 Year: 2017 Volume: 6 Issue: 4 Pages: 126-140
Publisher: Shiite Endowment ديوان الوقف الشيعي

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Abstract

Language Learning is a an indispensable part of human culture. Amajor purpose of language is to express thoughts, knowledge, andbeliefs through the processes of teaching and learning. As a result oflearning and functioning in a language, culture is sustained throughgenerations (Salili & Hossain,2007). At the same time, culture alsoinfluences how knowledge, teaching and learning are construed aswell as how teaching and learning are practiced. The knowledge isacquired in a particular cultural context transforms into schemata(mental representation of knowledge based on personal experience)that may not be universally acceptable. A better understandingof the interdependency of language and culture is paramountto the development of appropriate learning strategies to meet theneeds of an increasingly changing world. This paper examines theinfluence of culture on language learning in relation to the teachingand learning of critical thinking in the context of professional education.The increasing emphasis on critical thinking in education atall levels is applicable to any culture that is currently influenced bychanges in the global environment, but how culture influences theincreasing emphasis on critical thinking is not certain. Just as cultureaffects how people construe and practice teaching and learning(Merriam, 2007), culture can also have an important influenceon how critical thinking is perceived and exercised. The ability tothink well does not guarantee one’s ability to communicate effectively.Students are trained with critical thinking skills in order tosolve problems or make decisions; if they do not know how to presenttheir ideas appropriately, the efforts spent in critical thinkingwould be futile. While it is important to consider the language abilityof students, the manners and methods involved in the expressionof their critical thoughts should also be made explicit to others,regardless of their cultural backgrounds.


Article
Religious culture and Iraqi personality - Anthropological study
الثقافة الدينية والشخصية العراقية - دراسة انثروبولوجية

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Abstract

Religious culture is a collective cultural phenomenon in which a large number of individuals participate. They have many causes and factors, including political, economic and social. The researcher has introduced the natural factor as one of the factors influencing the religious culture because of its obvious impact on the Iraqi personality.It is therefore possible to say that the religious culture is the result of cultural and natural factors rooted and rooted in the city of Hilla since its establishment until the present, it clearly affects the population by reflecting on their personalities and behavior daily has tried to employ the direction of the school culture and personality in the subject of the study through the opinions and theories and pioneers This trend also employed the researcher interactive symbolic theory in the subject of the study to show the impact of symbols and religious activities and their spread among them because of the symbolic interaction between them.

الخلاصةتعد الثقافة الدينية ظاهرة جماعية ثقافية يشترك فيها عدد كبير من الافراد ولها أسباب وعوامل عديدة منها سياسي ومنها اقتصادي ومنها بسبب التنشئة الاجتماعية وقد أدخل الباحث العامل الطبيعي كأحد العوامل المؤثرة في الثقافة الدينية وذلك لأثره الواضح في الشخصية العراقية.وعليه يمكن القول ان الثقافة الدينية هي نتيجة لعوامل ثقافية وطبيعية متجذرة ومتأصلة في مدينة الحلة منذ تأسيسها وحتى الآن، فهي تؤثر بشكل واضح في سكانها من خلال انعكاسها على شخصياتهم وسلوكهم اليومي، فقد حاول الباحث توظيف اتجاه مدرسة الثقافة والشخصية في موضوع الدراسة في آراء ونظريات ورواد هذا الاتجاه وكذلك وظف الباحث النظرية التفاعلية الرمزية في موضوع الدراسة لبيان مدى تأثير الرموز والنشاطات الدينية وانتشارها بينهم بسبب التفاعل الرمزي الحاصل بينهم.


Article
Effect of Growth Regulators and Modified MS Medium on the Tissue Culture of Cucurbita pepo Plant
تأثير منظمات النمو ووسط MS المحور على الزراعة النسيجية لنبات قرع الكوسا Cucurbita pepo

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Abstract

Callus induction of the plant parts (leaves,stems,cotyledons, hypocotyls) of Cucurbita pepo on the solid medium enriched with different concentrations of auxins such as (2,4-D , IAA,NAA) and cytokinins such as (kin,BA). The medium (MS+1.0mg/L BA+0.5mg/L NAA)has been selected the best medium used to originate callus with an average estimated at 100%in the plant parts namely leaves ,stems and hypocotyls and the cotyledons respectively with an average 83% of callus ,and this was the highest percentage of the fresh weight amounte to 2.0 gm and stems callus at 1.2 gm.The study also succeded in originating callus of immature embryos with high percentage 85% on the medium (MS 0.5mg/L BA+0.1mg/L NAA) after it has been deduced on medium MS supported with 30mg/L 2,4-D for two weeks.Also the medium MS contained 1.5mg/L BA and 0.1mg/L NAA succeded in producing deduction percentage 100% from the cotyledons similary deduction media achieved a percentage ranged between 83-100% of cotyledons with out embryos.The results of altering the components of the selected MS medium (MS+1.0mg/L BA+0.5mg/L NAA)has shown deduction percentage amounted to 100% for the hypocotyls and 85% for the cotyledons when added 7ml/L of NH4NO3 instead of 4ml/L(MSN medium).Once we increased the amount of KNO3 compound up to 14ml/L instead of 8ml/L(MSK medium)on the same concentration of MS medium,the parts of cotyledons achieved the highest percentage of deduction 87%.Finally,the highest fresh weight of hypocotyls callus was noticed in this medium 1.8gm and the highest fresh weight of the cotyledons callus was noticed in MSN medium which was 2.0gm .Key words:Tissue culture,Cucurbita pepo, MS medium,NAA,BA.

استحدث كالس (الأوراق, السيقان, الأوراق الفلقية, السيقان تحت الفلقية) لنبات قرع الكوسا Cucurbita pepoعلى وسطMS الصلب المدعم بأنواع وتراكيزمختلفة من الاوكسينات (IAA, NAA, 2,4-D) والسايتوكاينينات (Kin, BA) وقد عدّ الوسط (MS + 1.0 ملغم/لتر BA + 0.5 ملغم/لتر NAA) أفضل الأوساط المستخدمة لاستحداث الكالس وبنسبة 100% من قطع الأوراق والسيقان والسيقان تحت الفلقية يليها قطع الأوراق الفلقية بنسبة استحداث 83% للكالس الذي سجل أعلى نسبة وزن طري بلغت 2 غم تلاها كالس السيقان 1.2 غم.كذلك نجحت الدراسة بالحصول على نسبة استحداث عالية 85% لكالس الأجنة غير الناضجة على وسط (MS + 0.5ملغم/لتر BA + 0.1 ملغم/لتر NAA) بعد تحفيز الأجنة على وسط MS المدعم بـ 30 ملغم/لتر 2,4-D لمدة أسبوعين, كما نجح وسط MS الحاوي على 1.5 ملغم/لتر BA + 0.1 ملغم/لتر NAA بإعطاء نسبة استحداث 100% من قطع الأوراق الفلقية وحققت أوساط الاستحداث نسبة 83-100% لقطع الفلق منزوعة الأجنة.أظهرت نتائج تحوير مكونات وسط MS المنتخب (MS + 1.0 ملغم/لترBA + 0.5 ملغم/لتر NAA) بإضافة 7 مل/لتر من NH4NO3 بدلا من 4 مل/لتر (وسط(MSN إعطاء نسبة استحداث للكالس بلغت 100% للسيقان تحت الفلقية و 85% للأوراق الفلقية وعند زيادة مركب KNO3 إلى 14 مل/لتر بدلا من 8 مل/لتر (وسط(MSK على نفس تركيز وسط MS المنتخب حققت قطع الأوراق الفلقية أعلى نسبة استحداث 87%, وقد لوحظ آن أعلى وزن طري كان1.8غم لكالس السيقان تحت الفلقية على هذا الوسط في حين سجل كالس الأوراق الفلقية أعلى وزن طري بلغ 2.0غم على وسط MSN. الكلمات المفتاحية: زراعة الانسجة، قرع الكوسا، وسط MS,NAA،BA .


Article
Evaluation the Response of four Potato Varieties (solanum tuberosum L.) to Growth under Salinity Stress Condition in vitro
تقييم استجابة اربعة اصناف من البطاطا Solanum tuberosum L.)) للنمو تحت ظروف الشد الملحي في خارج الجسم الحي

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Abstract

Abstract: In vitro effect of salinity in response of growth of four Potato varieties(Ambo, Arizona, Lusa and Rivera) were studied .Result showed difference in the response varieties for tissue culture, Where cultivar Lusa surpassed in response to the growth amounted to 100% of the shoot tips developing planted on the medium, Followed by product Arizona 90%while the response of Rivera was50%.Three concentrations of salinity levels used NaCl(8,10,12 dS/m) were used in addition to the treatment control (6 dS/m)to study the growth. The result showed that there was no significant differences among the varieties, but it was appeared significant differences among salt concentrations .The highest length of the branches was (4.90 cm) at 8 dS/m .The average number of branches at 12 dS/m significantly different from the others and gave the lowest rate (1.125 branch/plant).The number of nods at 10 dS/m gave the highest rate reached 4.125 nod/plant . According to the interaction between varieties and salt concentrations, Arizona gave the highest rate of stem length (5.85cm) and branches number (2.4 branch /plant) at 6 dS/m and Rivera variety gave the highest rate of number of nods which reached 5 nod/plant at 10 dS/m NaCl.

الخلاصة: درست استجابة النمو في خارج الجسم الحي لأربعة اصناف من البطاطا (Ambio, Arizona,Lusa,rivera) تحت ظروف الشد الملحي . اظهرت النتائج اختلاف استجابة الاصناف للزراعة النسيجية حيث تفوق الصنف Lusa في الاستجابة للنمو وبلغت 100%من القمم النامية المزروعة على الوسط الغذائي يليه الصنف Arizona90 % في حين كانت استجابة الصنف Rivera متوسطة وبلغت 50%، استخدم ملح كلوريد الصوديوم بثلاثة تراكيز وهي (8، 10، 12 دسي سيمنز/ م) اضافة الى معاملة المقارنة 6 دسي سيمنز / م لدراسة نمو العقل تحت ظروف الشد الملحي .اظهرت النتائج عدم وجود فروقات معنوية بين الاصناف في طول وعدد الافرع وعدد العقد , في حين أظهرت اختلافات معنوية بين التراكيز الملحية واعطى التركيز 8 دسي سيمنز/م اعلى معدل طول بالنسبة للافرع بلغ 4,90 سم اما بالنسبة لصفة معدل عدد الافرع فقد اختلف التركيز 12 معنويا عن التراكيز الباقية بحيث اعطى اقل معدل 1,125 فرعا / نبات , اما بالنسبة لصفة معدل عدد العقد ,فقد تفوق التركيز 10 دسي سيمنز/ م اذ أعطى اعلى معدل لعدد العقد بلغ ( 4,125 عقدة / نبات ) اما بالنسبة للتداخل بين الاصناف والتراكيز الملحية فقد تفوق الصنف Arizona في التركيز 6 دسم / م اذ أعطى اعلى معدل لطول وعدد الافرع وبلغ 5,580 سم و2,400 فرعا / نبات على التوالي, اما بالنسبة للتداخل فقد تفوق الصنف Rivera بالتركيز 10دسي سيمنز / م وأعطى اعلى معدل لعدد العقد بلغ ( 5 عقدة / نبات ) .


Article
RT PCR Detection and Propagation of Respiratory Syncytial Virus in Human Lung Carcinoma Cells (A549) cell line
تشخيص وتنمية فيروس Respiratory Syncytial Virusبتقنية سلسلة تفاعل البلمرة في خطوط خلايا سرطانية لرئة الانسان A549) )

Authors: Layla F. Ali ليلى فؤاد علي --- Raghad G. Al-Suhail رغد غالب السهيل --- Faisal G. Naser فيصل غازي ناصر
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2015 Volume: 56 Issue: 1A Pages: 69-74
Publisher: Baghdad University جامعة بغداد

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Abstract

In this study negative result of real-time reverse transcription-QPCR (RT-PCR) assay tests of Influenza virus of nasal screetion and throat swap samples of Iraqi patients hospitalized with signs and symptoms of an upper respiratory tract infection in Central Republic Health Laboratory in Iraq were tested for Respiratory Syncytial Virus infection by RT PCR .Positive samples was 4 out 0f 20 were used .Viral isolation was done on a monolayer of 70-80% confluent Human Lung Carcinoma Cells (A549) cell line and incubated at 33ºC for 4 days .Syncytia was observed in 3 positive samples

في هذه الدراسة تم اخذ 20 عينة من مسحات من افرازات الانف والحلق لمرضى عراقيين راقدين في المستشفيات واللذين يعانون من اصابات في الجهاز التنفسي والتي كانت نتائج فحص سلسلة تفاعل البلمرة الكمي QPCR RT- لفيروس الانفلونزا سالبة .تم فحص هذه العينات للكشف عن وجود اصابة بفيروس Respiratory Virus Syncytial بفحص سلسلة تفاعل البلمرة RT PCR في مختبر الصحة المركزي .كانت نتائج 4 عينات موجبة من مجوع العينات .تم عزل وتنمية الفيروس في خط خلايا رئة سرطانية للإنسان(A549) وحيث تم اصابة طبقة خلايا بكثافة %(80-70) وبعد فترة حضانة 4 ايام في 33 درجة مئوية تمت ملاحظة التأثيرات المرضية المتمثلة بتكون Synsytia في طبقة الخلايا لثلاث عينات موجبه.

Keywords

RT PCR --- RSV --- Tissue culture>


Article
Performance of VITEK 2 in the routine identification of bacteria from positive blood cultures in Sulaimani pediatrics’ hospital
أداء جهاز VITEK2 في الفحوصات الروتينية لتشخيص البكتريا من مزارع الدم الموجبة في مستشفى الاطفال في السليمانية

Author: Seenaa M. Ali سيناء محمد علي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2017 Volume: 58 Issue: 1C Pages: 435-441
Publisher: Baghdad University جامعة بغداد

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Abstract

Sepsis is a major cause of death worldwide among hospitalized patients, however, an accurate and advanced identification method is associated with improved sepsis patient survival. This Retrospective study carried out in Sulaimani pediatric teaching hospital from January 2014 to July 2015 and aimed to compare the effectiveness of VITEK 2 system with traditional manual procedures for identification of pathogenic bacteria in patients with a serious disease like sepsis. The positive blood cultures were divided into two groups; 138 positive cultures identified by conventional manual methods and 104 positive cultures identified by automated VITEK 2 system. The results showed that VITEK 2 system identified 16 genera and 30 species whereas only nine genera with seven species diagnosed by using routine method. The most important result during this study was the identification of five uncommon bacterial genera Kocuria, Leuconostic, Cedecea, Pantoea and Burkhoderia which have never been diagnosed in the microbiology laboratory of the hospital until the modern automated system VITEK 2 use. In conclusion, using VITEK 2 is required to enhance the performance of hospital’s microbiology laboratory which is essential for accurate diagnosis and prompt effective treatment of blood stream infections.

يعد تسمم الدم سببا رئيسا للوفاة بين المرضى الراقدين في المستشفى وان دقة التشخيص باستعمال طرق حديثة يكون مرتبطا بتحسين فرصة المريض للنجاة. اجريت هذه الدراسة في مستشفى الاطفال في السليمانية للفترة من بداية شهر كانون الثاني 2014 والى نهاية شهر حزيران 2016 .هدف الدراسة هوالمقارنة بين الجهاز الالي VITEK2 و الطرق اليدوية التقليدية المستعملة لتشخيص البكتريا المرضية للمرضى المصابين بتسمم الدم. شملت الدراسة تسجيل نتيجة التشخيص لكل الحالات التي اظهرت نتيجة زرع دم موجبة والتي قسمت الى مجموعتين: الاولى تضمنت 138 عينة شخصت بواسطة الطرق الروتينية والثانية تضمنت 104عينة شخصت بواسطة الجهاز الالي VITEK2. اسفرت نتائج التشخيص باستعمال VITEK2 عن تشخيص 16 جنس بكتيري و 30 نوع بينما لم تشخص الا 9 اجناس و 7 انواع باستعمال الطرق التقليدية. ان اهم نتيجة استحصلت من هذه الدراسة كانت تشخيص 5 اجناس لبكتريا غير شائعة (Kocuria, Leuconostic, Cedecea, Pantoea and Burkhoderia) والتي لم تشخض سابقا في مختبر المايكروبايولوجي ولكن شخصت فقط عندما بدأ العمل بنظام التشخيص الالي. نستنتج من هذه الدراسة ان استعمال جهاز VITEK2 ضروري لتحسين الاداء في قسم المايكروبايولوجي والذي يعد اساسيا للحصول على تشخيص دقيق يؤدي الى معالجة فعالة لحالات تلوث مجرى الدم.

Keywords

Sepsis --- VITEK 2 --- Blood culture

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