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Article
Study of micronuclei frequency in human lymphocyte of local samples in Eshtar village and Al-Tameem region surrounding at Al-Tuwaitha site

Author: Shatha K. K. , Amel J. Muttar , Abdullsahib K. Ali , Nayama H. Z. , Adil, H.E. , Yasser A.H.
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2017 Volume: 16 Issue: 3 Pages: 18-23
Publisher: Baghdad University جامعة بغداد

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Abstract

The present study aimed to use the biological techniques in a genotoxicity assessment of DNA damage in peripheral lymphocytes due to ionizing radiation which include micronuclus assy in peripheral lymphocytes of local samples in Esther Village and Al-Tameem region surrounding at Al-Tuwaitha site. This technique of the most important cellular genetic indicators that depend on the International Atomic Energy Agency (IAEA) for radiation dose assessment. This number included thirty samples from Eshtar village, aged (18 - 55 years), and thirty samples from Al-Tameem region, aged (18 - 50 years), also the control group, which included twenty samples aged (16 - 58 years) from other regions of Baghdad was studied. The results of the average of micronuclei showed no significant differences (p < 0.05) in peripheral blood lymphocytes of local samples in Eshtar Village and Al-Tameem region surrounding at Al-Tuwaitha site as compared with the control group. In conclusion, the recorded results revealed no significant differences in the rate of MN for population living in the Eshtar village, Al-Tameem region compared with the control groups. Also, the level of micronuclei in binucleated lymphocyte cells of the samples within was normal value according the technical report series No.405, IAEA in 2001.


Article
Electrical conductivity dosimetric characteristics of gamma-irradiated food salt

Author: Riyadh.Ch. Abul-Hail
Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2011 Volume: 37 Issue: 4A Pages: 25-29
Publisher: Basrah University جامعة البصرة

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Abstract

The electric conductivity characteristics of sodium chloride solution , have been studied in the present work considering its usage as a high dose dosimeter “accidental dosimeter” in the case of a nuclear fallout . Aqueous solution of gamma-irradiated food salt was investigated for dosimetric purpose using the electrical conductivity method. It was found that the solution containing [10-100]%(w/w) of food salt the most sensitive to radiation . The variation of electrical conductivity of all the solution was found to increase linearly with absorbed dose in the range (3-120) kGy. Reproducibility ,radiation dose response ,dose rate, and stability can have been studied.The results of this preliminary study show that food salt solution may be applied to a simple electrical conductivity detector for dose measurement in the dose range (3 – 120)kGy .

درست خصائص التوصيل الكهربائي لمحلول ملح الطعام بتركيز مختلفة من(10-100)%w/w لاستعماله لقياس الجرعة الإشعاعية العالية من أشعة كاما, حيث لوحظت استجابة جيدة للتوصيل الكهربائي مع الجرعة ضمن المدى3-120)kGy ) , كما درست خصائص التوصيل الكهربائي من ناحية عدم الاعتماد على معدل الجرعة والاسقرارية في درجة حرارة الغرفة . تشير هذه الدراسة إلى إمكانية استخدام محلول ملح الطعام كمقنن إشعاعي للجرعة العالية من kGy (3-120).


Article
Assessment of DNA damage in peripheral blood lymphocytes of workers occupationally exposed to low levels of ionizing radiation in Al-Amal hospital for cancer in Baghdad.

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Abstract

The present study aims to use the cytogenetic analysis as a biomarkers of professionals occupationally exposed to ionizing radiation in Al-amal National Hospital for cancer Management in Baghdad –Iraq, This study was carried out on Thirty Iraqi radiation workers exposed to a low dose of ionizing radiation, included twelve females and eighteen males between the ages of (22-57) years, as well as Twenty apparently healthy individuals collected randomly from population living Baghdad , aged (19 - 55) years which are non-smokers non- alcoholic as control group . The cytogenetic analysis including, chromosomal aberrations in lymphocytes, as fragment, ring and dicentric chromosomes also chromatid aberration , Micronuclei MN frequency, nuclear division index and mitotic index were performed on peripheral blood lymphocytes for workers and control group. The present study showed significant increase (p<0.05) in the chromosomal aberration for the worker as compared with the control group. Also there were found significant increase (p<0.05) in Micronuclei MN frequency in nucleated lymphocytes and nuclear division index for the worker as compared with the control group. While it was no significant differences in nuclear division index between worker and control group. Also there were found a significant increase (p<0.05) in comet tail length and tail moment values in the human lymphocyte in these radiation worker of studies as compared with the control group. The present study showes that the increase of chromosomal aberration and Micronuclei frequency, in hospital workers due to exposed to a low dose of ionizing radiation than in the controls. In conclusion, the results indicated that there is a possibility of using the changes in the chromosome aberration and micronuclei in human lymphocytes are as a useful as biodosimetric markers for the detection of human exposure to ionizing radiation. Also, the results obtained confirmed usefulness of the alkaline comet assay as a sensitive additional biomarker in the regular health screening of workers occupationally exposed to low doses of ionizing radiation. The current results of unstable chromosome aberration within of normal values according of the technical report of International Atomic Energy Agency (IAEA) No. 405, 2001.


Article
Measurement of Radioactivity in Flour and Macaroni Consumed in Basrah Governorate, Iraq and Evaluation of Gamma Dose Rates, Radiological Hazard Indices, Excess Life Time Cancer Risk and Ingestion Effective Dose

Authors: Abdalrahman Alsalihi1, --- Ali A. Abbas --- Riyadh Abualhail
Journal: Journal of Basrah Researches (Sciences) مجلة ابحاث البصرة ( العلميات) ISSN: 18172695 Year: 2017 Volume: 43 Issue: 2A Pages: 58-69
Publisher: Basrah University جامعة البصرة

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Abstract

The radioactivity levels of238U, 232Th, 40K and 137Cs were determined in 17brands offlour (6 brands) and macaroni(11 brands) consumed in Basrah, Iraq.This papershowed a comparison of the gamma absorbed dose rates (D),annual effective dose equivalent (AEDE)and the excess lifetime cancer risk (ELCR)for various types offlour and macaroni measured by SAM940-2G operating with BNC 2ʺx2ʺ gamma-ray NaI(Tl) detector along withthe thermoluminescencetechnique. For flour samples, the minimum specific activity values of 238U, 232Th, 40Kand 137Cs were 0.238±0.002 Bq/kg (at sample F1), 0.117±0.001 Bq/kg(at sample F4), 3.529±0.001 Bq/kg(at sample F4) and 0.040±0.007 Bq/kg(at sample F3) respectively, while the maximum values of the same isotopes were 0.325±0.002 Bq/kg(at sample F3),1.469±0.002 Bq/kg(at sample F5),102.348±0.001 Bq/kg(at sample F6) and0.179±0.003 Bq/kg(at sample F2) respectively. For macaroni samples, the minimum specific activity values of 238U, 232Th, 40Kand 137Cs were 0.195±0.002 Bq/kg(at sample M2),0.029±0.004 Bq/kg(at sample M1),40.390±0.001 Bq/kg(at sample M6)and0.01±0.008 Bq/kg(at sample M11)whereas the maximum values of the same isotopes were 1.430±0.002 Bq/kg(at sample M3),2.629±0.002 Bq/kg(at sample M11),294.495±0.001 Bq/kg(at sample M10) and0.566±0.002 Bq/kg(at sample M4).Various radiation hazard indices including the radium equivalent activity (Raeq), the ingestion effective dose (HT,r), the internal hazard index (Hin), the external hazard index (Hex), the gamma index (Iγ) andthe alpha index (Iα) have been determined for all 17 samples. Allachieved results have been found to be undertheinternationallimit standards. Thus, selected flour and macaronitypes are safe to be consumed in Basrah governorate. The findings of thisstudycould be used as a first step to create radiological baseline data of the hazard radiation in basic foodstuffsconsumed in Basrah/Iraq.


Article
ULTRAVIOLET RADIATION DOSIMETRY BY USING OF CN-85 , CR-39, LR-115 NUCLEAR TRACK DETECTORS
تجريع الأشعة فوق البنفسجية –UV باستخدام كواشف الأثر النووي CN-85 ، CR-39, LR-115

Authors: Firas M. Al-Jomaily فراس محمد علي --- Hussain A. Al-jobouri حسين علي الجبوري --- Ahmed K. Mheemeed احمد خلف محميد
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2012 Volume: 53 Issue: 3 Pages: 544-549
Publisher: Baghdad University جامعة بغداد

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Abstract

Ultraviolet radiation dosimetry was determined by using CN-85 , CR-39, LR-115 nuclear track detectors – NTD with measuring of ; number of track-NT , etching time-TB , nuclear track diameter - DT and etching velocity -VD . By this study appear the increasing in etching time-TB of NTD samples which irradiated by UV-radiation do not pure effect on the number of track - NT , comparing with un-irradiated samples . The increasing in UV-irradiation make decreasing in etching time- Topt for CR-39 , CN-85 , LR-115 nuclear track detectors with percent of 20% , 25% , 50% respectively , comparing with un– irradiated sample at the radiation dose 3.77x103 erg /mm2 . . The increasing of irradiation dose make increasing in nuclear track diameter-DT with increase in etching time-TB at the radiation dose 300x103erg / mm2 for LR-115 detector . This study showing there was increasing in the percent value of etching velocity -VB with increasing in radiation dose for CR-39 , CN-85 . The increasing in nuclear track diameter- DT with increasing in radiation dose was appearing as a resulted of energy of radiation and producted free radicals which interact with chemical etching solution . This study optioned by using nuclear track detectors CN-85 , CR-39 for determination the radiation dosimetry through measuring of etching velocity -VB butter than LR-115 detector . PACS:- 29.40-n,29.70-e,87.60.M

تم تجريع الأشعة فوق البنفسجية –UV باستخدام كواشف الأثر النووي CN-85 , CR-39، LR-115 وإيجاد معاملات الأثر النووي ، عدد الآثار NT - ، زمن القشط - TB , قطر الأثر –DT بالإضافة إلى سرعة نمو قطر الأثر-VD . وجد أن زيادة زمن القشط TB - مع التشعيع بأشعة UV- للكواشف لا يؤثر كثيرا على عدد الآثار مقارنة بالانموذج القياســـــــي غير المشعع . وان زيادة التشعيع تؤدي إلى نقصان الزمن الأمثل لظهور الآثارTopt- بنسبة %50 ،%25،%20 للكواشف CR-39 , CN-85 , LR-115 على التوالي بالمقارنة مع الانموذج غير المشعع و عند الجرعة 3.77x103 erg /mm2 . وتؤدي زيادة التشعيع إلى زيادة في أقطار الآثار- DT مع زيادة زمن القشط بعد الجرعة 300x103erg/mm2 للكاشف LR-115 . وتبين أن هناك زيادة تدريجية لسلوك سرعة نمو قطر الأثرVD- مع زيادة جرعة التشعيع للكواشف CN-85 ، CR-39, وعزيت الزيادة في قطر الأثر- DT مع زيادة التشعيع إلى الطاقة الناتجة من التشعيع ودور الجذور الحرة المتكونة وتفاعلها مع محلول القشط الكيماوي . وتبين إن هناك إمكانية استخدام كواشف الأثر النووي CR-39 , CN-85 أفضل من الكاشف LR-115 من خلال قياس نمو قطر الأثر VD - .

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