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Article
Evaluation of Lipids in Serum and Follicular Fluid on Oocyte and Human Embryo Quality after ICSI

Authors: Mustafa H.O. Hussein --- Qays A. M. Al-Khafaji --- Mufedah Ali Jawad --- Mohammad O. Selman --- et al.
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2017 Volume: 7 Issue: 1 Pages: 52-61
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The estimation of oocyte quality in human in vitro fertilization (IVF) is an important point for the embryologists. Oocyte selection and the identification of the best oocytes may help to limit an overproduction of embryos and to improve the results of oocyte cryopreservation programs.The follicular fluid (FF) can be provided easily during oocyte pick-up and known to represents an optimal source for non-invasive biochemical predictors of oocyte quality. However, till now no substance was found to be used as reliable markers of oocyte competence to fertilization, embryo development and pregnancy.Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with quality of oocyte and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised.


Article
Effect of Ovarian Stimulation Protocol on Embryo Quality in IVF-ICSI

Authors: Mufeda A. Jwad --- Ban A.Abdulmajeed --- Ula M. Al-Kawaz
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 1 Pages: 55-63
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Defects in reproduction that may involve both the male & femalepartners result in the inability to procreate which is known as infertility. Theuse of assisted reproductive technologies (ART’s) has been increasing over thepast three decades. Many efforts have been done to compensate for unsatisfactoryresults in in-vitro fertilization (IVF) procedures, so that patients undergodifferent ovarian stimulation protocols to control retrieval of multiple oocytesin a single cycle. Although ovarian stimulation has an important role in ART’s,it may also have detrimental effects on oocyte quality, embryo quality, and IVFoutcomes.Objectives: To assess the effect of long agonist versus antagonist protocols onoocyte and embryo quality in IVF- Intracytoplasmic sperm injection (ICSI).Patients,Materials and Methods:This comparative study was done on the embryos of seventy one infertile femaleswho were undergoing Intracytoplasmic sperm injection (ICSI) treatmentregardless to the presence or absence of previous trials. These patients weredivided into two groups, 45 of them treated with agonist protocol and other 26patients treated with antagonist protocol. First oocyte retrieved was used forfurther assessment. Of the 71 oocytes, there were eight immature oocytes (3 atgerminal vesicle (GV) stage, 5 at metaphase I (MI) stage) and three abnormaloocytes, and these were excluded from the following ICSI treatment. Assessmentand comparison of oocyte maturity, fertilization rate, cleavage rate and resultingembryo quality were done.Results:The results of the present study showed significant difference (p<0.05)in oocyte number and maturity but no significant (p>0.05) difference betweenthe group treated with long agonist and those on antagonist protocol regardingfertilization rate, cleavage rate, and embryo quality.Conclusions:The type of protocol used in in-vitro fertilization has no detrimentaleffect on oocyte or embryo quality.


Article
Evaluation of Soluble L-Selectin (CD 62L)Level in Embryo Culture Media to Embryo Quality and Implantation Rate

Authors: LubnaA. Al-Anbari --- Haider F. Ghazi --- Muayad S. Abood --- Alaa M. Hameed
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2018 Volume: 8 Issue: 2 Pages: 7-13
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

AbstractBackground:Although progress was made in an in vitro fertilization (IVF) techniques,the majority of transferred embryos fail to implant. Morphology embryoscoring is the standard procedure for most of IVF centers for choosing the bestembryo, but remains limited since even the embryos classified as (top quality)may not implant. Determining molecular mechanisms of human embryo implantationis an extremely challenging task due to the limitation of materials andsignificant differences underlying this process among mammalian species. L-Selectinand its ligand carbohydrate have been proposed as a system that mediatesinitial adhesion of human blastocysts to the uterine epithelia.Objectives:Quantitative evaluation of soluble L-Selectin molecule in an in-vitroculture media used for in vitro fertilization. Determine cut-off value of solubleL-Selectin quantity to be as qualitative predictor of successful implantationPatients and Methods: This prospective study was undertaken in the High Instituteof Infertility diagnosis and assisted Reproductive Technologies/ Al-NahrainUniversity/ Baghdad/ Iraq, during the period from September2017 to April2018.A total of 74 infertile women were underwent controlled ovarian hyperstimulation for intracytoplasmic sperm injection cycle. Flexible antagonist protocolwas used as ovulation induction protocol in all the cases .culture mediaL-Selectin level were measured on the day of embryo transfer by using Enzymelinked immune sorbent assay (ELISA) for all cases . Comparison in culture mediaL-Selectin was done to all cases.Results: There was no significant difference between pregnant and non-pregnantgroups in age , BMI, and level of basal hormone FSH, LH , AMH and E2 in theday of hCG injection, and significant difference in the level of L-Selectin inpregnant compared to non-pregnant groups.Conclusion: Level of L-Selectin concentration in embryo culture media can beused as predictor of ICSI success

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