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THE EFFECT OF CULTURAL AND ENVIRONMENTAL CONDITIONS ONBIODEGRADATION AND BIOSURFACTANT PRODUCTION BYSERRATIA MARCESCENS UTILIZING WEATHERED DIESEL OIL

Authors: Sahar Imad Mohammed --- Wameidh M. Potrus --- Nadem H. Hayder Gumaa
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2010 Volume: 13 Issue: 1 Pages: 112-120
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The capability of a Serratia marcescens isolate to biosurfactant production from spilled weathered diesel oil has been studied in batch culture. Several cultural and environmental conditions were analyzed to optimized condition for growth and biosurfactant production. Results showed that the optimized conditions which for growth was, pH 7, incubation period for 72 h, supplementation of the production medium with (NH4)2 SO4 0.4 % and weathered diesel oil in a concentration of 5 % which yielded 8.6 g/l biomass. Optimized condition for biosurfactant production was, pH 8, incubation period 96 h, (NH4)2 SO4 0.2 % and weathered diesel oil in a concentration of 6 % which yielded 10.5 g/l biosurfactant. Temperature 30 C was optimum for growth and biosurfactant production. Surface active properties of isolate studied during cultivation with weathered diesel oil at the concentration 6 % (w/v) at different incubation period. The isolate has synthesized extra cellular compounds which increase the E 24 % emulsion index of culture medium to 58 % and emulsification activity to 0.9. The presence of these substances (crude form) lower the surface tension of the culture until 43 mN/m. Bacterial cell surface hydrophobicity (BAH) as measured by analyzing cell affinity towards aliphatic, aromatic and mixed hydrocarbons was also determined. The isolate was found to have a surface hydrophobicity (Hydrocarbon affinity) in the following order: aliphatic, mixed and monoaromatic hydrocarbons. The present study concludes possibility of using surface active agents (Biosurfactant) produced by Serratiamarcescens mainly in: petroleum industry in enhanced oil recovery and in variety of biotechnological applications, including bioremediation of hydrocarbons contaminated sites.


Article
Crude oil characterization and hydrocarbon affinity of Amarah Oil Field, South Iraq
خصائص النفط الخام وعائديه النفط في حقل نفط العمارة، جنوب العراق

Authors: Abdul Salam Najeh Hasan عبد السلام ناجح حسن --- Salam Ismaeal Al-Dulaimi سلام أسماعيل الدليمي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2017 Volume: 58 Issue: 1A Pages: 103-114
Publisher: Baghdad University جامعة بغداد

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Abstract

Five oil sample of Mashrif and Nahr Umr Formation for Amarah oil field, southern Iraq, were taken and analyzed in Geo Mark laboratory in USA center in order to determine the bulk properties of crude oils and carbon isotopes for these samples in addition to determine biomarker parameters using Gas Chromatography(GC), and Gas Chromatography Mass Spectrometry) GCMS (analytical technique. According to these biomarker analyses of the two formation, it is indicated that they are non-degraded, marine, non-waxy, derived from carbonate source and deposition in anoxic marine environment. This study also showed that the bulk properties (terpanes and steranes) of Amarah oil field are one family, and the source rocks contain marine kerogen type II. All oil samples of Amarah oil field are from source rock refer to Middle Jurassic age of sargelu Formation.

تم اخذ خمسة نماذج نفطية لتكوين المشرف ونهر عمر من حقل نفط العمارة، جنوب العراق وتم تحليلها في مختبر (Geo Mark) في مركز الولايات المتحدة الامريكية من اجل تحديد خصائص المواد النفطية السائبة ونظائر الكاربون لهذه النماذج بالإضافة الى تحديد معايير العلامات البيولوجية باستخدام تقنيه (كروماتوغرافيا الغاز)، وتقنيه (كروماتوغرافيا الغاز- طيف الكتله). ووفقا لتحاليل العلامات البيولوجية للتكوينين فقد تبين انها غير متكتلة وبحرية وغير شمعية متكونة من صخور مصدرية كاربونية ومترسبة في بيئة بحرية غير مؤكسدة. كما اشارت هذه الدراسة الى ان الخصائص السائبة في حقل نفط العمارة تعود الى عائلة واحدة، وان الصخور المصدرية تحوي نوع الكيروجين البحري الثاني. كما ان جميع العينات النفطية هي من الصخور المصدريه التي تعود الى العصر الجوراسي لتكوين الساركلو.

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