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Article
Changes of immunological, Cytogenetic & Hematological Profiles in Chronic Myeloid Leukemia Treated with Imatinib Mesylate

Author: Almotessembellah Abdalruhman
Journal: Iraq Joural of Hematology المجلة العراقية لامراض الدم ISSN: 20728069/25432702 Year: 2014 Volume: 3 Issue: 1 Pages: 35-42
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Chronic myeloid leukemia (CML) (also known as chronic myelogenous leukemia, chronic granulocyticleukemia) is a clonal disease that results from an acquired genetic change in a pluripotential hemopoietic stemcell .Imatinib mesylate, is an inhibitor of the family of ABL kinases, these proteins serves a complex role in cellcycling and is important in lymphopoiesis, and has been shown to have impressive clinical activity in chronicmyeloid leukemiaObjectives: to assess the immunological, cytogenetic, and hematological parameters in patients with chronic myeloidleukemia on imatinib therapyMaterial and methods: Thirty one CML patients treated with imatinib mesylate at standard dosage were enrolled inthis prospective study. All patients were seen in the Department of hematology at the national center ofhematology /Almustansiriya University for six months from February to July 2012.Data on blood cell counts andblood film, serum levels of IgG, IgA ,IgM ,C3 and C4;assessed by radial immundiffusion method,Results: Twenty four out of thirty one CML patients treated with the imatinib regimen (those had done FISH study)reached a complete cytogenetic response. Reduction percent were 19.35% for IgG, 16.13% for IgM and 9.68%for IgA, six patients had IgG inferior to normal laboratory range, five patients presented a reduction of IgM andthree patients had IgA lower than normal range. No significant correlation of immunoglobulin levels compared toduration of treatmentConclusion: Imatinib mesylate can induce complete cytogenetic response in a high percent of CML patients. Cytogeneticresponse correlates well with duration of treatment .there is insignificant reduction of immunoglobulins levelspecially IgM. There is disturbance in the serum level of complement components (C3 &C4).There isinsignificant reduction in the absolute count of lymphocytes in correlation with duration of treatment


Article
Biochemical and immunological study of saliva in relation to oral health status in thalassemia major patients in Mosul

Authors: Mahmoud Y.M. Taha --- Raja H. Al-Jubori --- Ahmed S. Khudhur
Journal: Al-Rafidain Dental Journal مجلة الرافدين لطب الأسنان ISSN: 18121217 Year: 2011 Volume: 11 Issue: 3 Pages: 265-276
Publisher: Mosul University جامعة الموصل

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Abstract

Aims: The present study aimed to investigate some biochemical (Lysozyme and Peroxidase enzymes) and immunological (S-IgA) changes in saliva of thalassemia major patients and the correlation of these changes with the oral health status measured by the dmft/DMFT (decay, missing, filling tooth index for deciduous and permanent dentition), plaque and gingival indices.Materials and Methods: The study was carried out in Ibn Al-Atheer Teaching Hospital in Mosul and involved (91) subjects. The study group (70 thalassemia major patients) and the control group (21 normal non-thalassemic subjects). The study group was divided into two subgroups (35 each) according to the history of disease. Data was collected from each patient including medical status and oral health status indices and saliva samples were collected from each patient and stored at -20 oC to be analyzed for salivary Lysozyme by lysoplate method, salivary Peroxidase activity and salivary secretary S-IgA by ELISA.Results: Showed that the dmft/DMFT plaque and gingival indices in thalassemia major patients were significantly higher (P < 0.001) than normal subjects. The salivary lysozyme in thalassemia major patients was significantly lower (P < 0.001) than normal subjects. The salivary peroxidase activity was nearly higher in thalassemic patients than normal subjects. The salivary S-IgA in thalassemic patients was significantly lower (P < 0.001) than normal subjects. Conclusions: The study showed a significant correlation between the changes in some of the salivary constituents and the high prevalence of dental caries, plaque and gingivitis in thalassemia major patients


Article
Immunological Effect of Polysaccharide Extracted from Pseudomonas aeruginosa against Leishmania donovani in mice

Author: Hasna H. Jumah
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2006 Volume: 5 Issue: 3 Pages: 265-278
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Visceral leishmaniasis is a serious disease with high pathoogenicity. It was noticed that Polysaccharide extracted from Pseudomonas aeruginosa had the ability to induce both cellular & humoral immunity response against bacteria, fungi & parasites.OBJECTIVE:The aim of this study was to know the immunological effects of polysaccharide extracted from Pseudomonas aeruginosa before & after the infection of BALB/c mice with Leishmania donovani in trial to provide a method for prevention of the disease in human.METHODS:174 male BALB/c mice were divided into control & experimental groups with each group consisting of six animals. The experimental groups injected intraperitoneally (i.p) with 0.2 ml phosphate buffer saline (PBS) containing 1×108 parasite. Cerdain groups were injected intraperitoneally by polysaccharide (PS) at doses of 100, 150, 250 μg on day 3, 6, and daily for 6 days before infection with Leishmania donovani. These animals were sacrified after 8 weeks. Other groups were injected intraperitoneally with polysaccharides on day 7 post infection at the doses of 100, 150, 250, μg, 6 animals were sacrified after 2, 4, 6, 8 weeks. Total & differential count of leukocytes, measurement of spleen & liver weight and index, measurement of cellular immune response, histopathological changes liver and spleen were carried out.RESULTS:This study showed an increase in the total count of leukocyte accompanied by an increase in lymphocyte with decrease in monocyte percent in mice treated with polysaccharide before and after infection with Leishmania donovani which were statistically significant. Reduction in liver & spleen weights & their index in all treated groups was noticed before & after infection. The cellular immune response represented by delayed hypersensitivity has been enhanced in the mice treated with polysaccharide before &after infection. In treated groups, histopathological studies of mice showed hepatocyte hypertrophy, infiltration of the inflammatory cells in liver before & after infection. In spleen showed an increase in lymphocytes & monocytes in red pulp, while the infected mice (non treated) showed a necrotic foci, loss of architecture of both liver & spleen & obvious granulomatous inflammation in liver.CONCLUSION:Polysaccharidde extracted from Pseudomonas aeruginosa induced immunological activity by activation of cell mediated responses, stimulated an increase in WBC count & lymphocytes with minor histoptholgical changes in liver and spleen.


Article
Immune Effect of Viable and Heat Killed Lactobacillus acidophilus in Mice Infected with Salmonella typhimurium

Author: Zahraa K. Zedan
Journal: Al-Nahrain Journal of Science مجلة النهرين للعلوم ISSN: (print)26635453,(online)26635461 Year: 2012 Volume: 15 Issue: 4 Pages: 156-160
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

This study was designed to evaluate the immunological effect of viable and heat killed Lactobacillus acidophilus in mice infected with Salmonella typhimurium. The immunological parameters were total and absolute counts of leucocytes and phagocytic index of peritoneal cells. Twelve mice were divided into four groups. Each group consists of 3 mice. Group 1 was used as a negative control. Group 2 was infected with Salmonella typhimurium and use as positive control. Group 3 was fed with viable Lactobacillus acidophilus culture, and infected with culture of Salmonella typhimurium. Group4 was fed with heat killed Lactobacillus acidophilus culture, and infected with Salmonella typhimurium. Results indicated that treatment mice with viable Lactobacillus acidophilus were effective in enhancing the immune responses against Salmonella typhimurium as compared to negative (distilled water) and positive (Salmonella typhimurium) controls. While, mice fed with heat killed Lactobacillus acidophilus showed no enhancing immune effect on mice infected with Salmonella typhimurium

اجريت الدراسة لمعرفة التأثير المناعي لبكتريا acidophilus Lactobacillus المقتولة بالحرارة على الفئران المصابة ببكتريا السالمونيلاSalmonella typhimurium. درس التأثير المعزز المناعي الناتج من بكترياacidophilus Lb الحية والمقتولة بالحرارة من خلال دراسة التعداد الكلي والتفريقي لكريات الدم البيضاء وعملية البلعمة داخل الجسم الحي على 12 فأرة بيضاء والتي تم تقسيمها عشوائيا الى اربعة مجاميع 1 و2 و3 و4 وقد تضمنت كل مجموعة على ثلاثة فئران متساوية بالاعمار والاحجام. عدت المجموعة (1) التي تم تغذيتها على العليقة الاساس (مجموعة سيطرة سلبية)، اما المجموعة (2) ، ثم اصابتها بـ 0.1 مل من بكتريا S.typhimurium بعدد خلايا (2.5×10 7cfu/ml) ، بينما تم تغذية مجموعة (3) بـ 0.1 مل من بكترياacidophilus Lb الحية بعدد خلايا (1×10 9 cfu/ml )، وتم اصابتها بـ 0.1 مل من بكتريا S.typhimurium بعدد خلايا (2.5×10 7 cfu/ml) اما المجموعة (4) فقد تم تغذيتها بـ 0.1 مل من بكتريا acidophilus Lb المقتولة بالحرارة بعدد خلايا (1×10 9 cfu/ml)، ثم اصابتها بـ 0.1مل من بكتريا S.typhimurium بعدد خلايا (2.5×107 cfu/ml). اظهرت النتائج ما يأتي: التأثير المعزز المناعي في الفئران التي جرعت ببكترياacidophilus Lb الحية كان فعالا مقارنة مع مجموعة السيطرة بينما اظهرت الفئران المعاملة ببكترياacidophilus Lb المقتولة بالحرارة انخفاضا معنويا في التعداد الكلي والتفريقي لكريات الدم البيضاء وعملية البلعمة بالمقارنة مع مجاميع السيطرة.


Article
Micronucleus formation assay and phagocytic index in mice administered water leaf extracts of Camellia sinensis

Authors: Bushra H. Saleh بشرى صالح --- Rukaya M. Ebraheem رقية محمد ابراهيم --- Shahlaa M. Salih شهلاء صالح
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 / 24108057 Year: 2010 Volume: 52 Issue: 3 Pages: 355-358
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Green tea Camellia sinensis is non fermented tea. The tea is an infusion of flavorful leaves that has been consumed for centuries as a beverage and is valued for its medicinal properties. Over the last few decades green tea has been subjected to many scientific and medical studies to determine the extent of its long-purported health benefits, with some evidence suggesting regular green tea drinkers may have lower chances of heart disease and developing certain types of cancer. Green tea has also been claimed useful for weight loss management.Methods: Three doses of the hot water extract were used (19.2, 38.4 and 57.6 mg/kg) to investigate micronucleus formation and phagocytic index. Mice were divided into two groups. Group one; control and group two; mice were administered with three doses of the green tea extract orally as a single dose (0.1 ml) per day for 7 days. Then the mice were sacrificed in day 8 for immunological and cytogenetic assessments.Results: Results revealed that the first and third doses of green tea hot water extract were significantly effective in enhancing the values of phagocytic index and reducing micronucleus formation.Conclusions: Hot water may be a good solvent to extract active component from Camellia sinensis especially catechin, polyphenols, polysaccharides, flavonoids and vitamins. The high dose of hot water extracts of Camellia sinensis showed an excellent enhancing effects on the function of immune system of mice, moreover, reduction in micronucleus formation was recorded at such dose.Key words: Green tea, Cytogenetic, Immunological effect


Article
The Effect of Carrageenan on Lymphoma Patients under Chemotherapy (A case series study)

Authors: Israa A. Al-Mamory --- Fawaz D. Al-Aswad
Journal: Journal of Oral and Dental Research مجلة طب الفم والاسنان ISSN: 23106417 Year: 2017 Volume: 4 Issue: 2 Pages: 158-168
Publisher: Iraqi Association for Oral Research الجمعية العراقية لبحوث طب الفم

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Abstract

Background There are three types of carrageenan available (iota, kappa, and lambda) which differ in composition and degree of sulfation in polymeric structure. A malignant lymphocytes that accumulate in lymph nodes causes a group of diseases called Lymphomas causing the characteristics lymphadenopathy. These may pelt over into the blood or infltrate organs outside the lymphoid tissue. They are Hodgkin›s lymphoma and non–Hodgkin›s lymphoma and this is based on the histologic presence of Reed-Sternberg cells in Hodgkin›s lymphoma. Objectives The present study designated to determine the oral manifestations after application of carrageenan in patients with Hodgkin›s& Non-Hodgkin›s lymphoma receiving chemotherapy and to determine the effect of topical carrageenan on the levels of salivary immunoglobulin A, interleuekin-6 and tumor necrosis factor-α in Hodgkin›s & non-Hodgkin›s lymphoma patients. Results The oral manifestations was in Non-Hodgkin›s lymphoma patients (64%) and in Hodgkin›s lymphoma patients (36%) taste alteration, followed by burning mouth syndrome, dry mouth and lastly candidiasis with signifcant differences after application of carrageenan p>0.05.The levels of salivary immunoglobulin A, interleuekin-6 and tumor necrosis factor-α were slightly decreased but not signifcantly differences after carrageenan application. Conclusions The carrageenan effects on oral lesion may allow the use of Κ –carrageenan as a base or primary compound in dental practice besides the other compounds like chlorhexidine. The salivary immunological markers level slightly decreased in lymphoma patients after receiving carrageenan which indicate that there is no absorption from oral mucosa.


Article
STUDYING SOME IMMUNOLOGICAL PARAMETERS IN PREGNANT WOMEN WITH THYROID GLAND PROBLEMS
دراسة بعض المتغيرات المناعية عند النساء الحوامل المصابات باضطرابات الغدة الدرقية

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Abstract

This study was carried out to determine some hormonal and immunological parameters in women who attended Maternity and Children teaching hospital in Ramadi city , from 1st of August 2014 to 1st of April 2015. Ages of patients ranged from 16 - 49 years . The study included 358 blood samples from pregnant and non-pregnant women . sixty five samples were found to be abnormal thyroid function . The remaining 293 samples from healthy pregnant and not pregnan-pregnant women were used as a controls . Women under study were divided into three groups: Pregnant Women with thyroid disorder( Group I ) , Pregnant Women without thyroid disorder (Group II ,control 1) , and non- Pregnant Women without thyroid disorder (Group III , control 2). The study showed that there was no significant differences in T3 ,T4and FTI levels in patient compared with control 1 ( p-value >0.05) while it showed significant difference between patient and control 2 with p-value (<0.05). the means of T3,T4 and FTI in patient were 4.08 ng/ml, 91.09 μg/dl, 69.55 respectively. While there was no significant difference in level of TSH in cases and control 1 and control 2 with p-value >0.05. The mean level of TSH of patient was 18.65 μIU/ml . A comparison in Immunological parameters between the three groups with each other showed that there was significant increase in levels of Anti thyroxin peroxidase abs and Anti thyroglobulin abs in cases compared with control 2 with p-value <0.05,but didn’t show significant difference between cases and control 1 with p-value >0.05.The mean levels of patients for Anti TPO abs was 302.10 IU/ml and for Anti TG abs was 143.21 IU/ml.

تضمنت الدراسة تحديد بعض التغيرات الهورمونية والمناعية عند الحوامل المراجعات الى مستشفى الرمادي التعليمي للنسائية والاطفال للفترة من 1/8/ 2014 الى 1/4/2015 0 شملت الدراسة 358 نموذج دم من نساء حوامل وغير حوامل 0 وجد ان 65 امرأة حامل من بين العدد الكلي يعانين من بعض الاضطرابات بالغدة الدرقية بينما وجد ان العدد المتبقي لا يعانين من اي اضطراب 0 تراوحت اعمار النساء بين 16 – 49 سنة 0 شملت الدراسة ايضا العدد المتبقي من النساء اللائي لا يعانين من مشاكل بالغدة الدرقية والبالغ عددهن 293 0 قسم العدد الكلي للنساء قيد الدراسة الى ثلاث مجاميع : المجموعة الاولى شملت الحوامل اللائي ثبت معاناتهن من اضطراب الغدة الدرقية , والمجموعة الثانية شملت النساء الحوامل اللائي ثبت عدم معاناتهن من اضطراب الغدة كسيطرة اولى , اما المجموعة الثالثة فقد شملت النساء غير الحوامل ولا يعانين من مشاكل بالغدة الدرقية كسيطرة ثانية 0بينت الدراسة عدم وجود فروق معنوية في مستويات T3 و T4 و FTI عند مقارنة المجموعة الاولى بالثانية بينما تبين وجود فروق معنوية واضحه عند مقارنه المجموعة الاولى بالثالثةوجد ان معدلات مستويات T3 و T4 و FTI للمجموعة الاولى 4.08 ng/mlو 91.09 μg/dlو 69.55 ng/ml على التوالي , بينما تبين عدم وجود فروق معنوية في مستويات TSH بين المجموعة الاولى والثانية اذ بلغ معدل هذا الهورمون لدى المجموعة الاولى 18.65 μIU/ml 0عند مقارنة المتغيرات المناعية بين المجاميع الثلاثة تبين وجود فروق معنوية بمستويات الاجسام المضادة Anti thyroxin peroxidase و Anti thyroglobulin بين المجموعة الاولى والمجموعة الثالثة بينما لا يوجد فرق معنوي بين المجموعة الاولى والثانية , كما تبين ان مستويات هذين النوعين من الاجسام المضادة 302.10 IU/ml و 143.21 IU/ml. على التوالي 0


Article
Effect of Coriander seeds (Coriandrum Sativum) on some Haematological and Immunological Parameters of Awassi Ewes
تأثير إضافة بذور نبات الكزبرة Coriander Seeds للعليقة في بعض المعايير الدمية والمناعية للنعاج العواسية

Author: D. H. Y. Al-Zwein ضرغام حمزة يوسف آل زوين
Journal: Al-Anbar Journal of Veterinary Sciences مجلة الانبار للعلوم البيطرية ISSN: 19996527/27070603 Year: 2011 Volume: 4 Issue: 1 Pages: 92-102
Publisher: University of Fallujah جامعة الفلوجة

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Abstract

This study was conducted to find out the effect of coriander seeds as feed additive on some hematological and immunological parameters of Awassi ewes, This experiment was carried out in Animal farm- college of veterinary medicine Baghdad university from 1-3-2010 to 31-5-2010. Eighteen Awassi ewes at the age of 2-3 years were randomly divided in equal number into three groups (6 each)”body weight was considered”.Ewes in the first group kept as control and daily fed on concentrate diet (350 g/head) for three months, while the second group was fed the same concentrate diet contain (2.5%) coriander seeds, the third group was fed the same diet contain (5%) of coriander seeds. Also all animals were allowed to graze for 3-6 h/day. Blood samples were taken from jugular vein biweekly to determine, and white blood cells counts, differential Leukocytes counts, and the ratio of neutrophils /lymphocytes (N/L).Total serum protein, albumin and globulin concentrations were calculated). The result revealed that the second and third groups were significantly (P<0.05) recorded higher values in their white blood cells counts and lymphocytes counts also lymphocyte and Eosinophiles ratios, Total protein, globulin, compared with the control group. While the second and third groups were also recorded significantly (P<0.05) lower values in their neutrophils and monocytes ratios and neutrophile lymphocyte ratio (N/L) and albumen concentration compared with the control group. It was concluded from this study that coriander seeds could be used as feed additives with diet for improving animal health and some haematological and immunological parameters).

هدفت الدراسة معرفة تأثير إضافة مستويين من بذور نبات الكزبرة Coriander Seeds كإضافات علفية في رفع الاستجابة المناعية الدمية للنعاج العواسية. أجريت هذه الدراسة في الحقل الحيواني - كلية الطب البيطري/ جامعة بغداد، ابتدأت من 1/3/2010 ولغاية 31/5/2010 لقياس الاستجابة المناعية الدمية كل أسبوعين، أستخدمت ثلاث مجاميع من النعاج العواسية الناضجة(2-3) سنة والتي قسمت عشوائيا (بمعدل 6 نعاج لكل مجموعة) مع الأخذ بنظر الاعتبار وزن الجسم الحي، عوملت النعاج كالآتي المجموعة الأولى (سيطرة) غذيت على 350 غم/ رأس عليقة مركزة يوميا، أما المجموعة الثانية أضيفت لنفس العليقة 2.5% بذور الكزبرة والمجموعة الثالثة أضيفت لنفس العليقة 5.0% بذور الكزبرة مع الرعي الحر لكل المجاميع جمعت عينات الدم من الوريد الودجي للنعاج وعزلت المصول لأجراء الفحوصات الدمية، (عد كريات الدم البيض الكلي، عد كريات الدم البيض التفريقي، نسبة الخلايا العدلة/ اللمفاوية N/L، قياس مستوى البروتين الكلي، الألبومين، الكلوبيولين).أظهرت نتائج الدراسة تفوق المجموعتين المغذاة على أضافات الكزبرة معنويا )0.05 (P<على مجموعة السيطرة في معدلات أعداد كريات الدم البيض، الخلايا اللمفاوية، نسبة الخلايا اللمفاوية، الحمضة (عد كريات الدم البيض التفريقي) تركيز البروتين الكلي، الكلوبيولين. وأشرت المجموعتين المغذاة على إضافات الكزبرة انخفاض معنوي على مجموعة السيطرة في معدلات (نسبة الخلايا العدلة، الاحادية (عد كريات الدم البيض التفريقي) نسبة الخلايا العدلة/ اللمفاوية، تركيز الالبومين. يستنتج من هذه الدراسة أمكانية أستخدام بذور نبات الكزبرة مع عليقة الاغنام كإضافات علفية والذي أدى الى تحسن حالة الحيوان الصحية وزيادة كريات الدم البيض وتركيز البروتين الكي والكلوبيولين


Article
EXPERIMENTAL Trichophytone Mentogrophytes Var Mentogrophytes INFECTION IN RABBITS: CORRELATION BETWEEN IMMUNOLOGICAL AND CLINICAL OBSERVATION.
الإصابة التجريبية لفطر Trichophyton mentogrophytes var mentogrophytesفي الأرانب: دراسة العلاقة بين المناعة والعلامات السريرية.

Author: Nawzat Abuzaid Issa نوزت ابوزيد عيسى
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2009 Volume: 8 Issue: 2 Pages: 173-180
Publisher: Basrah University جامعة البصرة

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Abstract

To questing the severity and courses of experimental infection with Trichophyton mentogrophytes var mentogrophytes in four groups of eight rabbits, prepared HIS against the fungus was used in different routs and doses in two groups (second and third group). 0.2ml subcutaneously in 2nd group and0.1 ml interadermally in 3rd group. The shorter course of the infection was recorded in 1st group previously infected and left with out treatment with HIS, while complete healing was recoded after 5 weeks and 8 weeks in 2nd and 3rd group respectively in compared to 4th group ( control group) after 10 weeks.

استهدفت هذا الدراسة لمعرفة الضراوة و فترة العدوى التجريبيةِ لفطر Trichophyton mentogrophytes var mentogrophytes في ثمانية أرانبِ مقسمة الى اربعة مجاميع، استخدمت المصل المنيع المهيء ضدّ الفطرِ بجرعة 0.2 مليلتر تحت الجلد في المجموعةِ الثانيةِ و بجرعة 0.1 مليلترِ في الادمة في المجموعةِ الثالثةِ. اما المجموعة الاولى كانت مصابة سابقا بنفس عترة الفطرو شفيت تلقائيا بدون اعطاء المصل المنيع, اما المجموعة الرابعة استخدمت كمجموعة السيطرة.حيث لوحظ شفاء فترة الاصابة قصيرة جدا فى المجموة الاولى اما في الحيوانات المجموعة الثانية بعد 5 اسابيع و 8 اسابيع في المجموعة الثانية و الثالثة على التوالي مقارنة مع المجموعة السيطرة بعد 10 اسابيع.


Article
Efficacy of immunological tests in diagnosis of acute human brucellosis

Authors: Ali A. Najum Al-Zhaidy*; --- Jawad K.Tarrad Al-Khafaji --- Abdul Kareem S. Al-Yasari
Journal: Karbala Journal of Medicine مجلة كربلاء الطبية ISSN: 19905483 Year: 2006 Volume: 1 no. 1 Pages: 81-89
Publisher: Kerbala University جامعة كربلاء

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Abstract

The study was carried out in Al-Hashimyia hospital, Babylon governorate for theperiod from May, 2005 to May ,2006 to evaluate the results of some immunologicaltests in diagnosis of acute phase of brucellosis in human populations. A total of 410patients with suspected brucellosis were admitted to the hospital with age-groupranged from 18 to 65 years. 150 patients were studied, as documented cases withacute brucellosis , and 150 healthy persons were involved as control group. Numerousimmunological tests like ELISA test , Rose- Bengal test , standard tube agglutinationtest and E-rosette test were used to demonstrate cellular and humoral immuneresponse of infected host . ELISA test was the best and accurate test in diagnosis ofthe acute infection, followed by standard tube agglutination test . All cases of acutebrucellosis showed a high number of active rosette –forming cells.

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