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Article
Isolation of Some Bacteria from Chronic Otitis Media

Author: Samira Kgeer Jreemich سميرة غجير جريمخ
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2014 Volume: 10 Issue: 18 Pages: 159-163
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Chronic Otitis media is a very common disease in people of Diwania due to several factors , microbiological , envirnmental and social . Swab samples and data were collected from private surgeries of specialized doctors ( ENT ) . 75 samples were collected , 51 cases were positive ( 68%) and 24 cases were negative ( 32%). Number and percent of infected males 28 ( 54.9%) , while number and percent of infected females 23 ( 45.1%) .The main results showed high percents of infection were in ages between 20-29 years old and 30-39 years old( 31.4 % and 25.5% ) respectively . Six types of bacteria were isolated from the patients , two of them were gram positive ( Staphylococcus aureus and Streptococcus pyogens ) while gram negative were four ( Pseudomonas aeruginosa , Proteus vulgaris , E. coli and Enterobacter cloacae ) . The highest percent of gram positive was S. aureus 90.5% , and the highest percent of gram negative was P.aeruginosa 56.6% ) .Sensitivity test was done to know the best antibiotic can be used as treatment for otitis media . The most effective antibiotics for most causative agents were cefotaxin , co-trimaxozole , gentamycin , tetracycline.

أن مرض ألتهاب الأذن الوسطى المزمن من الأمراض الشائعه بين الناس وذلك بسبب عوامل متعدده منها أحيائيه , بيئيه وأجتماعيه . جمعت نماذج من الأذن المريضه للمراجعين للعيادات المتخصصه بالأذن والأنف والحنجره عدد الحالات 75 حاله منها 51 حاله موجبة لألتهاب الأذن الوسطى (68%) و 24 حاله سالبه ( 32%) . عدد الحالات الموجبة من الذكور 28 ( 54.9%) والحالات الموجبة من الأناث 23 (45.1%) . بينت النتائج الرئيسيه نسب عالية من الأصابه في الأعمار بين 20 – 29 سنه و 30- 39 سنه ( 31.4 % و 25.5 % ) على التوالي , فضلا ً عن نسب عالية سجلت في الذكور ( 54.9 % ) أكثر من النساء . تم عزل ستة أنواع من البكتريا من المرضى , أثنين منها كانت موجبة لصبغة كراموهي ( Staphlococcus aureus , Streptococci pyogens ) بينما البكتريا السالبة لصبغة كرام كان عددها أربعة وهي ( Pseudomonas aeruginosa , Proteus vulgaris , E. coli and Enterobacter cloacae ) . أعلى نسبة بين البكتريا الموجبة لصبغة كرام كانت S. aureus ( 90.5 % ) بينما أعلى نسبة بين البكتريا السالبة لصبغة كرام كانت P. aeruginosa ( 56.6% ) .تم أجراء أختبار الحساسية للمضادات الحيوية وذلك لمعرفة أفضل هذه المضادات لتستعمل كعلاج لألتهاب الأذن الوسطى . أغلب المضادات التي كانت ذو تأثير جيد على البكتريا هي ( Cefotaxin , Co-trimaxozole , Gentamycin, Tetracyclin ).


Article
Re-evaluation of lunation of the activity of somantibacterial drugs against clinical isolates of Escherichia coli in al- najaf al- ashref governorate

Authors: Adel H. Sheeh عادل شيح --- Saad B. Nashtar سعد نشتار
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2010 Volume: 6 Issue: 1 Pages: 21-27
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Escherichia coli is one of the most important bacterial pathogen that can cause several disease to human being . In our study we try to investigate the sensitivity resistance pattern of Escherichia coli against three antibiotics ( Amikacin, Nalidixic acid and Cephalexin). Methods: For this purpose we collected 51 clinical isolates of Escherichia coli from stool and urine of outpatient and inpatient patients from different wards of AL-SADER Teaching Hospital in AL-NAJAF AL-ASHRAf, IRAQ, and tested by culture and sensitivity test . Results: The results appeared that Amikacin show the highest percentage of sensitivity ( 66.66 % ) , while Cephalexin show the lowest percentage of sensitivity ( 6.34 % ) .Conclusions: we conclude that Amikacin is the most sensitive antibacterial drug for Escherichia Coli as compared with Nalidixic acid and Cephalexin, while Cephalexin is the most resistant antibacterial drug for Escherichia Coli as compared with Amikacin and Nalidixic acid.


Article
Biodegradation Effect of some Bacterial Isolates on some Endocrine Disruptors (EDCS)

Author: Amna M.A. Al-Hashimi
Journal: Al-Mustansiriyah Journal of Science مجلة علوم المستنصرية ISSN: 1814635X Year: 2018 Volume: 29 Issue: 2 Pages: 43-49
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Endocrine disruptors [EDCs] raised a certain concern for living health began since last century, via interfere with natural hormone functions and produce reversible or irreversible biological effects. Bisphenol (BPA) is an organic compounds that causing human health risks. Different bacterial spp. has biodegradation ability for wide range of EDC. Twenty water samples were collected from different area around Baghdad city. Four bacterial isolates were isolated included [Pseudomonas aeruginosa, Pseudomonas luteola, Proteus penneri, and Escherichia coli]. All isolates were characterized morphologically and biochemically. The effect of substrate tolerate bisphenol (BPA) [5 mg/ ml] using well diffusion method were investigated. The biodegradation effect of bacterial isolates on breakdown BPA and its derivatives using UV vis spectrophotometer were studied and comparing in various incubation time and temperatures to assess the effect of physical conditions on bacterial ability of BPA degradation. P. penneri showed a significant ability to resist para-phenol and meta-phenol, while highly sensitive to ortho-aminophenol and paracresol. P. aeroginosa was sensitive to para and meta- aminophenol, while resist to degraded phenol compounds ortho aminophenol and para cresol]. P. luteola was resistance for all phenolic compounds, while E.coli showed sensitivity for para cresol only. Biodegradation effect data showed a significant effect for P. luteola after 15 days of incubation followed by P. penorri and E.coli. to degrade phenolic compounds. Data demonstrated that P. luteola has an obvious degradation effect for BPA after 15 days of incubation. However, P. aeruginosa showed an absolutely different behavior toward BPA which showed an raising absorbance after 15 days of incubation. The aim of this study is to identify the ability of different local bacterial isolates to breakdown the phenol compounds and its derivatives in surface water. This has certain impact on the water purification and industry to provide safe water for consumers.

أثارت المواد الكيميائية المسببة اختلال الغدد الصماء مخاوف معينة بشأن صحه الانسان والتي بدأت منذ القرن الماضي،تأثر هذه المواد على وظائف الهرمون الطبيعي ومسببه آثار بيولوجية قابله للالنعكاس على صحه الإنسان والكائنات الحيه] . مركب البسفينول هو مركب عضوي يسبب مخاطر على صحة الإنسان. جمعت 20عينه من الماء من مناطق مختلفه من بغداد. تم اختياراربع عزلات بكتيريه لأجراء اختبارات االقدره على تحليل المركبات الفينوليه [ Pseudomonas aeruginosa, Pseudomonas luteola, Proteus penneri, and Escherichia coli ]. شخصت جميع العزلات البكتريه على الصفات المظهريه و الفحوصات البايو كيمائيه . تمت دراسة تأثير البسفينول [5 ملغم / مل] باستخدام طريقة النشر على العزلات البكتريه. كما وتم دراسة تأثير التحليل الحيوي للعزلات البكتيرية على البيسفينول ومشتقاته باستخدام جهاز المطياف الضوئي للأشعة فوق البنفسجية ومقارنت النتائج في ظروف مختلفه [ مده الحضن ودرجة الحرارة] لتقييم تأثير الظروف الفيزيائية على قابليه العزلات البكتيريه المنتخبه على تحليل البيسفينول. أظهرت بكتريا P. penneriقدرة كبيرة على مقاومة para-phenol and meta-phenol، في حين كانت حساسه للغاية ل ortho- aminophenol and para- cresol. بينما كانت بكتريا P. aeruginosaحساسه لل وpara aminophenol meta-phenol بينما كانت مقاومه لمركبات الفينول الاخرى ortho aminophenol, and para cresol.. في حين اظهرت بكتريا Ps. Luteola مقاومه عاليه لكل مركبات الفينول. بينما اظهرت بكتريا E.coli حساسيه للpara- cresol فقط. وأظهرت بيانات تأثير التحلل البيولوجي تأثير كبير لبكتريا Ps. luteolaبعد 15 يوما من الحضانة تليه اProteus penneri و E.coli. اهميه الدراسه هي امكانيه تطبيقها واستخدامها في مجالات صناعه وتنقيه المياه والتقنيات الحيويه لتوفير حياه افضل للانسان. الهدف من هذه الدراسة هو التعرف على قدرة انواع مختلف من البكتيريا المعزولة محليا على تحليل مركبات الفينول ومشتقاته المثبطه لعمل الغدد الصماء.


Article
PREPARATION OF VACCINE FOR DIABETIC FOOT PATHOGENIC BACTERIA USING LOW LEVEL DIODE LASER
تحضير لقاح مضاد للبكتريا المرضية المسببة لإلتهاب القذم السكري بإستخذام التشعيع بالليزر

Author: Zainab Awad Radi زينب عواد راضي
Journal: Basrah Journal of Veterinary Research. مجلة البصرة للابحاث البيطرية ISSN: Print:18138497 E; 24108456 Year: 2012 Volume: 11 Issue: 1 Pages: 189-196
Publisher: Basrah University جامعة البصرة

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Abstract

Since the invention of laser in 1960, lasers have been developed and approved in many fields. Lasers can now be regarded as practical tools with unique properties that have been utilized effectively in several applications in fields of medical and biological sciences. six bacterial isolates were isolated from human samples of diabetic foot infections, which used for preparation of vaccines. The experiment was conducted on fifteen adult male rabbits, they were divided into three groups with 5 rabbits each. After irradiation of the bacterial suspensions with the diode laser for different exposure times and different frequencies, and the wavelengths used were (660, 820, 915 ) nm, the growth of bacterial isolates decreased until killed of bacteria at ( 40) min. The aim of the current study was to preparation of vaccines (live attenuated and killed) by irradiation of the bacteria by the low level diode laser. Wavelength (660) nm was more effective in killing the bacteria, and the variations were not significant between the live attenuated and the killed vaccine


Article
Preparation of Vaccine against Diabetic Foot Pathogenic Bacteria Using Low Level Diode Laser

Author: Zainab Awad Radi (M.Sc) a Ihsan F. Rostum (M.Sc, PhD) b *Sadiq Abbas Al-Mukhtar (FICMS) c
Journal: Al-Kindy College Medical Journal مجلة كلية الطب الكندي ISSN: 18109543 Year: 2013 Volume: 9 Issue: 2 Pages: 29-31
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Since the invention of laser in 1960, lasers have been developed and approved in many fields. Lasers can now be regarded as practical tools with unique properties that have been utilized effectively in several applications in fields of medical and biological sciences.Objectives: The aim of the current study was to preparation of vaccines (live attenuated and killed) by irradiation of the bacteria by the low level diode laser.Methods: six bacterial isolates were isolated from human samples of diabetic foot infections, which used for preparation of vaccines. The experiment was conducted on fifteen adult male rabbits; they were divided into three groups with 5 rabbits each. Blood samples were collected from the marginal ear vein of the rabbits after one month of the vaccination, for the purpose of measuring the concentration of the immunoglobulins which present in their serum, using Radial Immunodiffusion (RID) method by specialized kits (LTA-Italy).Results: After irradiation of the bacterial suspensions with the diode laser for different exposure times and different frequencies, and the wavelengths used were (660, 820, 915) nm, the growth of bacterial isolates decreased until killed of bacteria at 40 min. The results of IgA concentrations for the three groups were highly significant (P < 0.01) when comparing the attenuated with control group, while were significant (P < 0.05) between the killed vaccine and control groups, and not significant variations between the attenuated and killed groups,Conclusions: Wavelength (660) nm was more effective in killing the bacteria, and the variations were not significant between the live attenuated and the killed vaccine.


Article
Association of Pathogenic Bacterial Isolates in Burn Wound Infections

Author: Abbas Atiya Hammoudi
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2014 Volume: 11 Issue: 1 Pages: 52-57
Publisher: Babylon University جامعة بابل

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Abstract

Burn wound infection is a major medical problem in all areas of the world. One hundred fifty burn wound swabs were studied from 100 patients admitted to Al-Karama teaching hospital in Baghdad from 1/ 11/ 2007 to 1/ 4/ 2008.Fourty two ( 42%) of patients were males and fifty eight ( 58 %) were females. The age group of patients rang was from one year to 50 years. The high incidence of burn patient was 36 ( 36 % ) at the age group 10-19 years in both sex and the low incidence was 2 ( 2% ) at the age group 50 years in both sex. According to the causes of burn ; 50 (50 % ) were due to flame , 41 (41 % ) were due to scald, 7 (7% ) were due to electrical burns and 2 ( 2 % ) cases were due to chemical burns. Out of 195 (100 %) bacterial isolated, 103 (52.8 %) were isolated in pure culture while 92 (47.2%) were mixed culture .The most dominant bacteria were Klebsiella SPP 36 (18.5%) followed by Pseudomous aeuginosa 19 (9.7%),Staphylococcus aureus 15(7.7%), Candida albicans 11(5.6%), Escherichia coli 8(4.1%), Proteus mirabilis 6(3.1%), Staphylococcus epidermidis 4(2.1%) ,Enterococcus faecalis 2(1%) and Streptococcus pyogens 2(1%)

تعد اصابات الجروح احد المشاكل الطبية الكبيرة في العالم.تم اخذ 150 مسحة من 100 مريض في مستشفى الكرامة في بغداد للفترة من 1/11/2007 الى 1/4/2008 توزعت عينة الدراسة بواقع 42 من الذكور و58 من الإناث بمدى عمر من 1-50 سنة اخضعت عينات المسحات الى الزرع البكتيري وكانت النتائج ان البكتيريا الاكثر ظهورا كانت الكلبسيلا بنسبة 18.5 % تليها الزوائف الزنجارية والعنقوديات الذهبية.


Article
EFFECT OF PURE LIGNAN FROM FLAXSEEDS (LINUM USITATISSIMUM) ON INDUCED AND ANTIMUTAGENIC ON BACTERIAL SYSTEM
دراسة تاثير مركب اللكنان المنقى من بذور نبات الكتان على احداث ومنع الطفرات الوراثية في النظام البكتيري

Author: عصام فاضل الجميلي1
Journal: Iraqi Journal of Biotechnology المجلة العراقية للتقانات الحياتية ISSN: 18154794 Year: 2010 Volume: 9 Issue: 3 Pages: 564-577
Publisher: Baghdad University جامعة بغداد

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Abstract

The study aimed to compare pure lignan compound with methotraxete (MTX) drug on three bacterial isolates (Bacillus ssp-.(G3), Arthrobacter spp-(G12) and Brevibacterium spp. (G27)). In addition, mutagen (MTX) was compared a standard mutagen which called (NTG). The mutagen MTX has higher mutation effect than NTG on the bacterial isolates. The lignan compound showed no effect at 50 and 75 g/ml concentrations but at 100 g/ml of lignan was caused the mutagen resistance to streptomycin for treated isolates 383.3, 166.6 and 166.6 mutation/ml for G3 , G12 and G3 isolates respectively. This means the lignan concentrations have the inhibition effect on bacterial isolates which result in dead ratio ranged between 1.9– 54 % on the G3 isolates.

يهدف البحث إلى مقارنة مركب اللكنان المنقى من بذور نبات الكتان Linum usitatissimum مع المطفر MTX في حث الطفرات على العزلات البكتيرية Bacillus spp.(G3)،Arthrobacter spp.(G12) وBrevibacterium spp.(G27) كما وتمت مقارنة للمطفر MTX مع أحد المطفرات والتي تستعمل كنموذج دراسي في التطفير NTG وللعزلات البكتيرية قيد الدراسة، إذ وجد إن المطفر MTX قد فاق تاثير المطفر NTGفي تطفير الخلايا للعزلات البكتيرية قيد الدراسة، أما مقارنة تأثير مركب اللكنان مع المطفر MTX فقد وجد إن التراكيز 50 و 75 مايكروغرام/مليلتر لم يكن لها أي تأثير تطفيري في الخلايا البكتيرية، بينما التركيز 100 مايكروغرام/مليلتر سبب ظهور طفرات مقاومة للستربتومايسين وللعزلات الثلاث إذ بلغت 383.3 و 166.6 و 166.6 طفرة/مليلتر للعزلات G3 و G12 و G27 على التوالي. كانت تراكيز مركب اللكنان لها تأثير تثبيطي نوعاً ما في الخلايا البكتيرية إذ سبب نسب قتل متفاوتة تراوحت بين 1.9-54% سيما في العزلة G3 .


Article
Hydatidosis of slaughtered sheep in Baghdad City; bacteriological study of infected hydatid cyst fluid

Author: Waleed AT Al-Ani
Journal: Mustansiriya Medical Journal مجلة المستنصرية الطبية ISSN: 20701128 22274081 Year: 2012 Volume: 11 Issue: 2 Pages: 45-48
Publisher: Al-Mustansyriah University الجامعة المستنصرية

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Abstract

Background: Hydatid cyst is an infection caused by cestode species ofEchinococcus granulosus and often localized in viscera of animals andhumans. Hydatid cyst is typically filled with a clear fluid (hydatid fluid)that is sterile bacteriologically. Bacterial infection of hydatid fluid issometimes present, but not always and this leads to sterilizing the cystand prevents producing protoscolicies.Aims: The present work aims to study the prevalence of hydatidosis insheep slaughtered in Al-Shuala and Al-Karkh abattoirs in Baghdad cityand isolation of the possible bacteria that infect hydatid fluid of hydatidcyst.Materials and Methods: A 6-months period observation of two slaughterslabs of sheep (condemned livers), inspection was done in the sameslaughter slabs to assess the hydatid cyst and application ofbacteriological examination with culture on the present hydatid cystfluid.Results: The study showed that the prevalence of hydatidosis was 124(9.9%) of 1258 examined livers. Of the 124 hydatid livers, 32 (25.8%) werefertile and the rest were sterile and calcified. A bacteriological study offertile hydatid cysts has be done under sterile conditions to isolation ofbacteria , of the thirty two fertile cyst cultured in this study, 18 (56.3%)were infected with different types of bacterial isolates includingEscherichia coli (38.9%), Klebsiella spp. (27.8%), Staphylococcus spp.(25.0%), Proteus spp. (11.1%) & Pseudomonas spp. (8.2%).Conclusions: the prevalence of hydatidosis in addition to bacterialinfection in liver isolated from sheep seriously affects the keeping qualityof liver as an important food production and important to the health ofthe consumers.


Article
Efficiency of the bacteria Bacillus thuringiensis isolate from different local soil as bio insecticides on different insects in Iraq
تقييم كفاءة عزلات بكتريا Bacillus thuringiensis المعزولة من ترب محلية على بعض الافات الحشرية في العراق

Authors: Hussam Aldin A.M. Salih حسام الدين عبدالله حمد --- Jasser M. Jamil جاسر محمد جميل
Journal: Tikrit Journal for Agricultural Sciences مجلة تكريت للعلوم الزراعية ISSN: 18131646 Year: 2014 Volume: 14 Issue: 2 Pages: 214-221
Publisher: Tikrit University جامعة تكريت

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Abstract

Soil survey was conducted during the years 2005 and 2006 for seven Iraqi Governorate representing different climatic and soil stricter for the presence of Bacillus thuringiensis . The results indicated that the best isolation method was the inhibitor with thermal treatment 51.2% of total presence bacterial. The total average of bacterial presence is representing Iraqi soil was 34.5%. The highest presence of bacteria was 50% for Diala and Baghdad. Mortality eate of the bioassay isolation test showed higher 56.7% for Anbar , Baghdad for Tribolium confusum concentration 1.2 x 106 spor / ml. The result of bioassay of Bacillus thuringiensis isolates against Culex pipiems showed mortality 96.9% in Sulimaiyah and Anbar with 1.2 x 105 spor / ml concentration . The bioassay isolates testing spodoptera littoralis showed mortality rate of 96.6% in Diala and Anbar with 1.2 x 106 spore / ml.

اجريت مسوحات ميدانية عام 2005-2006 لترب سبع محافظات عراقية مثلت التباين المناخي والاختلاف في نسجة التربة حيث شملت ارض جبلية في الشمال وسهلية في الوسط والجنوب وديمية بالغرب وشاطئية وحدائق بالوسط لتحري عن البكتريا الممرضة للحشرات Bacillus thuringiensis . اظهرت نتائج العزل ان الطريقة الثانية المتضمنة التحفيز بوجود المثبط ثم المعاملة الحرارية هي الافضل . وجدت البكتريا Bacillus thuringiensis في 20 نموذج من مجموع 39 نموذجاً فحصت اي بنسبة 51.2% ، بينما الطريقة الاولى كانت بالمرتبة الثانية فقد وجدت البكتريا Bacillus thuringiensis في 13 نموذجاً من مجموع 39 نموذجاً فحصت وبنسبة 33.3% . اما الطريقة الثالثة فلم نحصل على بكتريا Bacillus thuringiensis من خلالها الا من ستة نماذج فقط من مجموع 39 نموذجاً اي بنسبة 15.3%. التقييم الحيوي للعزلات اظهر تفوق عزلة الانبار وبغداد وذي قار بالتركيز 1.2 × 106 بوغ / مل حيث بلغت نسبة القتل 56.7% على حشرة الخنفساء الصدئية المحيرة Tribolium confusum . واما تقييم كفاءة العزلات على البعوض Cluex pipiens pipiens فلقد تفوقت عزلات محافظتي الانبار والسليمانية حيث بلغت نسبة القتل 96.6% وبالتركيز 1.2 × 105 بوغ / مل. وتقييم العزلات على دودة القطن Spodoptera littoralis فقد تفوقت عزلات الانبار وديالى اذ بلغت نسبة القتل 96.6% بالتركيز 1.2 × 106 بوغ / مل.


Article
Chlamydial Antibodies , Pro-Inflammatory Cytokines and Bacterial Significance among Infertile Women

Authors: Abd-Alnabi Jouiad Abid --- Afrah Jawad Al-Zwaid
Journal: Medical Journal of Babylon مجلة بابل الطبية ISSN: 1812156X 23126760 Year: 2015 Volume: 12 Issue: 4 Pages: 943 -947
Publisher: Babylon University جامعة بابل

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Abstract

The current study was done to investigate the relation between the bacteria different kindsandinfertility in women, the study include 108 specimen from infertile and fertilewomen with ages range (20-40years) these specimens collected from infertility and gynecology center of Maternity and Pediatrics Teaching Hospital in Babylon province. Urine, vaginal swabs and blood samples were used in this study. Bacteriological diagnosis for urine and vaginal swabs accomplished and showed variation in types and number of isolates.The isolates can be distributed as follows:Gram positive bacterial isolates include Staphylococcus aureus 48 (30%) 18 isolates urine and 30vaginal swabs, Escherichia coli24urine and 19 vaginal swabs43(26.8%). Female infertility divided according to their causesinto different types and the detection by anti-Chlamydia trachomatis antibodies reveals high concentration of IgG (46%) in the women with Polycystic ovary syndrome group,while the second high concentration (36%) in women with primary infertility . Immunological test of Cytokine IL-10 in patient show decreased in their concentration 11.5pg/ml compared with healthy at P< 0.05 especially at polycystic ovary syndrome group.

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