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Article
Oocyte Quality and Embryonic Development after Oral Administration of Speramax® in Female Mice as Experimental Model for Mammals

Authors: Saad S. Al-Dujaily --- Hiba S. Hamza
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2014 Volume: 4 Issue: 2 Pages: 6-13
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

Background:Speramax® was found to play an important role in sperm function characters andmales reproductive performance with no studies on its effects on the oocytematuration and embryonic development in females.Objective:The goal of the present work was to examine the effect of Speramax ® onoocyte maturation ,ova quality , embryonic development and live birth usingmice as a model for mammals .Materials and Methods:In this study, Speramax ® was administrated orally for 1, 2 and 4 weeks . Onehundred and ten female mice were randomly divided into four groups , the firstgroup was superovulated(SUO) while the second group was treated by Speramax®with SUO and the third group was spontaneously ovulated (SPO) and treated withSperamax ® and the forth group was spontaneously ovulated (SPO ) and served ascontrol group .Another forty- eight female mice were used for the determination ofthe number of live births .Results: The results indicated that treatment with Speramax® showed a positiveeffect on oocytes maturation in vivo. There was a highly significance ( p˂ 0.0001)improvement in the number of mature oocytes following treatment withSperamax® in SPO and SUO mice compared with SPO and SUO mice not treatedwith Speramax® . The percentage of embryonic development after 24 and 48 hoursof mating in treated groups with Speramax® was significantly ( p˂ 0.05) higher thanSPO and SUO mice .The study showed that the quantity and quality of embryosobtained from the treated groups were superior to that of the untreated group .Conclusions:It was concluded that the Speramax® greatly improved oocyte maturation,early embryonic development and embryo grading quality embryos with anincrease in the numbers of mice live births.


Article
Evaluation of Lipids in Serum and Follicular Fluid on Oocyte and Human Embryo Quality after ICSI

Authors: Mustafa H.O. Hussein --- Qays A. M. Al-Khafaji --- Mufedah Ali Jawad --- Mohammad O. Selman --- et al.
Journal: Iraqi Journal of Embryos and Infertility Researches المجلة العراقية لبحوث الأجنة والعقم ISSN: eISSN: 26166984 / pISSN: 22180265 Year: 2017 Volume: 7 Issue: 1 Pages: 52-61
Publisher: Al-Nahrain University جامعة النهرين

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Abstract

The estimation of oocyte quality in human in vitro fertilization (IVF) is an important point for the embryologists. Oocyte selection and the identification of the best oocytes may help to limit an overproduction of embryos and to improve the results of oocyte cryopreservation programs.The follicular fluid (FF) can be provided easily during oocyte pick-up and known to represents an optimal source for non-invasive biochemical predictors of oocyte quality. However, till now no substance was found to be used as reliable markers of oocyte competence to fertilization, embryo development and pregnancy.Metabolism and ATP levels within the oocyte and adjacent cumulus cells are associated with quality of oocyte and optimal development of a healthy embryo. Lipid metabolism provides a potent source of energy and its importance during oocyte maturation is being increasingly recognised.


Article
Low oocyte quality related with the aging ewes
تقدم العمر للاغنام وعلاقته بالانخفاض النوعي للبيوض

Authors: Abbas Musa Ali عباس موسى علي --- Saad Akram Hatif سعد اكرم هاتف
Journal: The Iraqi Journal of Veterinary Medicine المجلة الطبية البيطرية العراقية ISSN: 16095693 Year: 2013 Volume: 37 Issue: 2 Pages: 261-265
Publisher: Baghdad University جامعة بغداد

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Abstract

This study was conducted to know the effect of ewe age on oocyte quality as well as the relations between oocyte viability and normal uterine condition. Eighty three (83) reproductive systems of non-pregnant ewes were collected from Al-shulla abattoir. The Total oocytes were aspirated from right ovaries reached 61.45% and 38.55% from left ovaries. Immediately after aspiration, the oocytes were examined by light microscopic and conceded as mature if surrounded completely with cumulus oopherus. While the stained oocytes by trypan blue were conceded as dead oocytes and excluded. According to ewes age the oocytes were classified into (3) groups, the first group ranged between 1-2 years, second group 3-6 years and the third group over 6 years. The total oocyte collection from these groups was 20, 23, 40 oocyte. The results indicated that 14 oocytes (70%), 17(73.91%) and 10(25%) from groups 1, 2, and 3 with cumulus cells, respectively. While the total live oocyte reached to 60. Normal endometrium was observed in 90%, 95% and 80%for 1,2and 3 groups respectively. It was concluded from this study that aged ewes showed low quality oocyte with infertile endometrium.

صممت هذه التجربه لدراسة تاثير عمر النعاج على نوعية البيوض و كذلك العلاقة ما بين الرحم وحيوية البيوض .جمعت 83 عينة من الجهاز التناسلي الانثوي من مجزرة الشعلة . كانت نسبة البيوض المجمعة من المبايض اليمنى 61.45 % , اليسرى38.55% قسمت البيوض اعتمادا على عمر النعاج الى ثلاثة مجايع عمرية , تراوحت اعمار المجموعة الاولى 1-2 سنة , والمجموعة الثانية3-6 سنوات و المجموعة الثالثة اكثر من 6 سنوات. اما عدد البيوض فكان 20 , 23 و40وعلى التوالي بويضة بتسلسل المجاميع . عند الفحص بلغ عدد البيوض المحاطة كاملا بخلايا الركمة المبيضية 14 في المجموعة الاولى و17 في المجموعة الثانية و10 بيوض في المجموعة الثالثة. بلغ عدد البيوض الحية الكلي (60) اما نسبة الارحام الطبيعية فكانت في المجموعة الاولى 90% و95% في المجموعة الثانية و80% في المجموعة الثالثة . وقد استنتج من الدراسه ان افضل عمر لجمع البيوض من النعاج مابين 3-6 سنوات كما بين الفحص المجهري لطبقات الرحم ان الاصابات الرحميه تزداد بعد السنه السادسه من عمر النعجه فضلا عن عدم وجود فرق احصائي بمستوى (P>0.05) في عدد الخلايا الحية والميتة بينما كان هناك فرق احصائي (P<0.01) في البيوض المحاطة بشكل كامل بالخلايا الركمة في المجموعة 1و2 مقارنه المجموعه 3. نستنتج من هذه الدراسة ان الاغنام المسنة تكون بيوضها اقل نوعية وبطانة رحم قليلة الخصوبة

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