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Article
The association between acne vulgaris and Tumor Necrosis Factor- alpha gene promoter polymorphism at position-308

Author: Hamadi Hadi Abttan Al-Hilali حمادي عبطان الهلالي
Journal: Al-Qadisiyah Medical Journal مجلة القادسية الطبية ISSN: 18170153 Year: 2014 Volume: 10 Issue: 17 Pages: 86-95
Publisher: Al-Qadisiyah University جامعة القادسية

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Abstract

Results; genotyping of TNF-α revealed three genotypes; the wild homozygous GG type, the heterozygous GA & the mutant homozygous AA type, the genotype frequency for these three types in acne patients was (29%, 67% & 4%) respectively, in control group, however, it was (58%, 30% & 12%) respectively. The frequency for GA type was found statistically significantly increased in acne patients compared to the healthy controls (P<0.001), significant association was found between the minor A allele and females patients(40.6%) compared to healthy females(28.8%) ,P value 0.001,also all the female that had severe and very severe acne carry the GA genotype. Background ;This study was carried out on (100) patients (64 females & 36 males) with different severity of acne vulgaris with an age range(11-40 )year, seen in AL-Diwaniya Teaching Hospital/ Department of Dermatology, from December 2011 to February 2012, another (50) apparently healthy subjects were taken as a control group. Materials; Blood samples were collected from both groups, genomic DNA was extracted from peripheral blood leukocytes for further molecular study, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used .

أجريت هذه الدراسة على (100) مريض من مرضى حب الشباب بمختلف درجات الشدة (64 إناث و 36 ذكور) من الفئة العمرية (11-40 سنه) من مراجعي قسم الأمراض الجلدية في مستشفى الديوانية التعليمي للفترة من كانون الأول 2011 حتى شباط 2012, إضافة إلى (50) شخص سالم ظاهريا كمجموعة تحكم.تم جمع عينات من الدم لاستخراج الحمض النووي من الكريات البيض لكلتا ا لمجموعتين للكشف عن وجود أي ارتباط بين تعدد الأشكال الجينية لعامل نخر الورم-ألفا في الموقع 308 من المنطقة المروجة والاستعداد للاصابة بحب الشباب باستخدام تفاعلات البلمره المتسلسلة وتقنية الأجزاء المتكسرة المتعدد باستخدام إنزيم NCOI والتي تعطي أجزاء ذات حجوم جزيئيه مختلفة تعبر عن الأصناف الجينية المختلفة .أظهرت تقنية الأجزاء المتكسرة المتعدد ثلاثة اصناف جينية لعامل نخر الورم-الفا في الموقع 308 وهي: صنف متماثل اللواقح GG, والصنف المتخالف GA, ونوع متحول متماثل اللواقح AA وكان تردد هذه الأصناف الجينية الثلاثة عند المرضى كالاتي :(29% , 67% و 4%) على التوالي, إما في مجموعة الاصحاء فقد كانت كالاتي :(58%, 30% و 15%) على التوالي.وجدت الدراسه ان هناك فرق احصائي مهم بين الصنف الجيني من نوع GA بين مرضى حب الشباب مقارنة مع الاصحاء P < 0.001)). اضافة الى العثور على ارتباط مهم بين الاليل A عند الاناث المرضى(40.6%) مقارنة بالاناث الاصحا(28.8%) اضافة الى ان جميع النساء المصابات بحب الشباب الشديد وجدن انهن حاملات للنوع الجيني GA.نتيجة هذه الدراسه تشير الى ان تعدد الاشكال الجينيه لعامل نخر الورم من الممكن ان تسهم في زيادة الاستعداد للاصابه بحب الشباب مع ملاحظة وجود ارتباط مهم بين الاليل A الطفيف وشدة المرض عند الاناث.


Article
Detection of Toxoplasma Antibodies and TNF-α in Rheumatoid Arthritis Patients Treated with Methotrexate
التحري عن الاجسام المضادة للمقوسات الكونيدية و عامل التنخر الورمي في مرضى التهاب المفاصل الرثياني المعالجين بالميثوتريكسيت

Authors: Rasha H. Kuba رشا حسين كبة --- Khawla H. Zghair خوله حوري زغير --- Mohammed H. Alosami محمد هادي العصامي
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2014 Volume: 55 Issue: 4A Pages: 1535-1540
Publisher: Baghdad University جامعة بغداد

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Abstract

This study was conducted to evaluate the prevalence rate of toxoplasmosis among 294 rheumatoid arthritis (RA) patients treated with methotrexate (MTX), 50 RA patients without treatment and 50 samples as healthy control. Blood samples were collected and the presence of T.gondii IgG and IgM antibodies was determined by using Enzyme linked immunosorbent assay (ELISA). Tumor necrosis factor alpha (TNF-α) was also estimated in serum of all subjects by using ELISA method too. The seroprevalence of toxoplasmosis IgM and IgG in RA+MTX was 60(20.408%), and 98(33.33%), in RA patients 4(8%), and 18(36%) while, it was 2(24%), 6(12%) in healthy group. Tumor necrosis factor alpha (TNF-α) was also estimated in serum of all subjects by using ELISA method too. The mean levels of TNF-α in seropositive anti-Toxoplasma IgM and IgG of RA+MTX patients were 3.781 pg/ml ± 0.571) and (36.98 pg/ml ± 0.58), in RA patients (25.404 pg/ml ± 1.748) and (40.12 pg/ml ± 1.7) while, they were (5.04 pg/ml ± 0.643) and (10.7 pg/ml ± 1.7) in healthy group. The results showed significant difference (P<0.05) was found between treated and untreated patients.

أجريت هذه الدراسة لتقييم معدل انتشار داء المقوسات الكونيدية في294 مريض بمرض التهاب المفاصل الرثياني المعالجين بالميثوتركسيت, و50 مريض بمرض المقاصل التهاب الرثياني غير معالجين و50 عينة كعينات سيطرة. تم جمع العينات وتحديد وجود الأجسام المضادة لطفيلي المقوسات الكونيدية من النوع (ج) و (م) باستخدام تقنية الاليزا. كذلك تم قياس عامل التنخر الورمي بتقنية الاليزا أيضا. وكانت الأجسام المضادة لطفيلي المقوسات الكونيدية من النوع (ج) و (م) في مجموعة التهاب المفاصل الرثياني المعالجين بالميثوتركسيت 60(20.408%)و 98(33.33%), ولمرضى التهاب المفاصل الرثياني غير المعالجين 4(8%)و 18(63%) أما في مجموعة السيطرة 2(24%)و6(12%). وكان معدل مستوى عامل التنخر الورمي في الأشخاص المصابين بطفيلي المقوسات الكونيدية من النوع (ج) و (م) في مجموعة التهاب المفاصل الرثياني المعالجين بالميثوتركسيت ( (3.871pg/ml±0.571و (36.98pg/ml±0.58), ولمرضى التهاب المفاصل الرثياني غير المعالجين (25.404 pg/ml ±1.748), (40.12 pg/ml ± 1.7) وفي مجموعة السيطرة 0.643) (5.04 pg/ml ±, و (10.7 pg/ml ± 1.7). أوضحت النتائج وجود فروق معنوية (P<0.05 ) بين المرضى المعالجين وغير المعالجين .


Article
Effect of the Biological Drug Etanercept on Tumor necrosis factor-α Levels in Psoriatic Patients

Authors: Basman Medhat Fadheel --- Ban Noori Al-Qadhi --- Rasha Hussain Kuba
Journal: Iraqi Journal of Science المجلة العراقية للعلوم ISSN: 00672904/23121637 Year: 2018 Volume: 59 Issue: 2C Pages: 998-1005
Publisher: Baghdad University جامعة بغداد

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Abstract

Psoriasis is a common, chronic, immune mediated disorder.The disease is arising as a result of dysregulated interactions of the innate and adaptive immune system in the context of skin epithelium and connective tissue. The biological drug Etanercept(ETN) approved for use in treated psoriasis. ETN is tumor necrosis factor- α (TNF-α) inhibitor. In this study, 48 psoriatic patients were taken before and after treatment who attended to the Dermatology and Venereology Department in Baghdad Teaching Hospital during the period from December 2016 to September 2017and 50 samples were used as healthy control group. The results showed that most psoriatic patients 52.08 % were within the second and third decades 20-35 year, and the majority of psoriatic patients were males 62.5% and the ratio of male to female is 1.67:1. Moreover, the results demonstrated that the males were more expected psoriasis compare with females. Blood samples were collected and TNF- α was estimated in sera of all subjects by using Enzyme Linked Immunosorbent Assay (ELISA). The TNF- α mean levels in psoriatic patients before treatment was 189.5±26.0 ng/ml, and after treatment was 223.6±41.1 ng/ml compar with the healthy control group 93.5±2.4 ng/ml. The results showed significant differences between the studied groups


Article
The Role of IL-1 and Tumor Necrosis Factor-α in Pathogenesis of Rheumatoid Arthritis

Authors: Eman Sh.Al-Obeidy --- Shatha F. Abdullah
Journal: Iraqi Academic Scientific Journal المجلة العراقية للاختصاصات الطبية ISSN: 16088360 Year: 2011 Volume: 10 Issue: 2 Pages: 161-165
Publisher: The Iraqi Borad for Medical Specialization المجلس العراقي للاختصاصات الطبية

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Abstract

ABSTRACT:BACKGROUND:Rheumatoid Arthritis (RA) is a common chronic inflammatory and destructive arthropathy that cannot be cured.Interlukin-1 (IL-1), IL-6 and tumor necrosis factor (TNF- α) are the key cytokines that drive inflammation in the disease.OBJECTIVE:The study was established to shed light on the possible role of IL-1 and TNF- α in pathogenesis of RA.PATIENTS AND METHODS:Rheumatoid Factors (RF),TNF-α and IL-1 were detected by Enzyme-Linked Immunosorbent Assay (ELISA) .C-reactive protein(CRP) on the other hand has been detected by using latex agglutination kit in the serum of 50 Iraqi patients with RA in comparison with 50 healthy individuals.RESULTS:Significant high levels of IL-1and TNF- α were observed in RA patients sera with P-value ( 0.04 and 0.001) respectively compared to healthy control group, in addition a positive linear association was found between the concentration of IL-1and TNF- α and CRP with P-value ( 0.05 and 0.001) respectively.CONCLUSION:IL-1and TNF- α play a role in pathogenesis of RA


Article
THE PROTECTIVE EFFECTS OF FELODIPINE ON METHOTREXATE-INDUCED HEPATIC TOXICITY IN RABBITS

Authors: Noor H. Swayeh نور حسون صويح --- Ahmed R. Abu-Raghif احمد رحمه ابو رغيف --- Ban J. Qasim بان جمعة قاسم
Journal: IRAQI JOURNAL OF MEDICAL SCIENCES المجلة العراقية للعلوم الطبية ISSN: P16816579,E22244719 Year: 2016 Volume: 14 Issue: 2 Pages: 166-173
Publisher: Al-Nahrain University جامعة النهرين

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Background:Methotrexate is folic acid antagonist, used in the therapy of various types of diseases. Oxidative stress and inflammation have the major role in methotrexate toxicity.Objective:To study the protective effects of felodipine against methotrexate-induced hepatotoxicity in rabbits.Methods:Twenty four rabbits divided randomly into three groups. Group I was left without treatment, group II received a dose of 20 mg/kg methotrexate (MTX) intraperitoneally from 3rd day for three successive days and group III received a dose of 0.5 mg/kg felodipine orally for 7 days in addition to MTX therapy similar to group (2). On 8th day the following parameters (liver enzymes, liver tissue homogenate of glutathione, malondialdehyde and tumor necrosis factor-α) were monitored. As well as assessment of histological changes on liver tissue sections after scarification.Results:Administration of felodipine significantly decreased the elevated levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), malondialdehyde and tumor necrosis factor-alpha. It also elevated glutathione levels significantly, with improvement of histological features related to MTX exposure in group III compared with group II.Conclusion: Felodipine can protect hepatic tissue against MTX-induced hepatotoxicity.Keywords: Hepatotoxicity, Methotrexate, Felodipine, Glutathione, Malondialdehyde, Tumor Necrosis Factor-alpha.


Article
Cytokine Profile in Patients with Rheumatoid Arthritis

Author: Falah S. Manhal* PhD د. فلاح سالم منهل
Journal: Journal of the Faculty of Medicine مجلة كلية الطب ISSN: 00419419 Year: 2009 Volume: 51 Issue: 4 Pages: 433-436
Publisher: Baghdad University جامعة بغداد

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Abstract

Summary:Background: Cytokines produced by inflammatory cells play a pivotal role in synovial inflammation and joint destruction in rheumatoid arthritis.Patients and Methods: The cytokine serum levels were measured by EASIA (Enzyme amplified sensitivity immunoassay) in sera from 50 RA patients, and 40 healthy donors. Cytokine levels were compared in different RA subpopulations (positive or negative rheumatoid factor (RF), long term or recent onset disease, high or low disease activity). In addition, the possible association with other demographic and clinical parameters (gender, age, etc) was also analyzed. Results: It was demonstrated that IL-2, IL-6 and IFN-δ levels were elevated in serum samples of RA patients as compared with apparently healthy controls. Maximum elevation of TNF-α was recorded in a few number of patient's sera. There were non significant differences between control and RA patient groups in serum TNF-α level. Conclusions: Assessing the serum IL-2, IL-6, IFN-δ and TNF-α levels may be helpful in the confirmation of the RA activity. Due to the chronic course of this disease, other inflammatory markers must be identified in order to provide early therapeutic strategies to these patients.Key words: Rheumatoid Arthritis, Interleukine-2, Interleukine-6, Tumor Necrosis Factor- α, Interferon- δ.


Article
The effect of intracrevicular injection of fucose on serum interlukine -1beta and tumor necrosis factor alpha

Authors: Chenar A. Mohammad --- Khlood A. Al-Safi خلود الصافي --- Bakhtiar M. Ahmed
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 2 Pages: 125-129
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: α-L-Fucose is a methyl pentose sugar, had the ability to kill bacteria, controlling infection and normalizeimmune function. The objective of this study is to determine the effect of sulcular injection of fucose on rabbitsperiodontium , throughout measuring the level of some proinflammatory cytokine ; interlukine 1beta ( IL-1beta) andtumor necrosis factor ( TNF-alpha) in sera of rabbits before fucose injection and at 3 days after fucose injection.Materials and Methods: The study was carried on using ( 20 ) male rabbits of the same species weighted (1-1.5 kg ) ,the blood samples were collected from hearts of 20 rabbits before fucose injection and consider as( non injectedgroup) , and after 3 days of fucose injection and consider as (fucose injected group) and analyzed fordetermination of the concentration of IL-1ß and TNF-α.Results: The results showed a highly significant decrease in the mean concentration of TNF-α in sera of fucoseinjected group(19.05± 1.166) pg ⁄ml when compared to its mean concentration in non injected group (27.25±7.371)pg ⁄ml ,also a highly significant decrease in the mean concentration of IL-1ß in sera of fucose injected group(34.19 ±3.1)pg ⁄ml as compared to its mean concentration in sera of non injected group (38.86 ±4.565)pg⁄ml.Conclusion: both proinflammatory cytokines were influenced and inhibited by local fucose injection


Article
Effect of orthodontic tooth movement on salivary levels of Interleukin-1beta, Tumor Necrosis Factor-alpha, and Creactive protein

Authors: Noor Saadi --- Nidhal H. Ghaib نضال حسين غايب
Journal: Journal of baghdad college of dentistry مجلة كلية طب الاسنان بغداد ISSN: 16800087 Year: 2013 Volume: 25 Issue: 4 Pages: 120-125
Publisher: Baghdad University جامعة بغداد

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Abstract

Background: Orthodontic force is considered to stimulate cells in the periodontium to release many mediators suchas cytokines which play a responsible role for periodontal and alveolar bone remodeling, bone resorption and newbone deposition. Aim of this study was carried out to estimate changes of the (interleukin-one beta, tumor necrosisfactor – alpha and C-reactive protein) levels in unstimulated whole saliva during the leveling stage of orthodontictooth movement.Materials and methods: The sample consisted of thirty adult patients (12 males and 18 females) with ages ranges (19-23) years. Each sample had Class I and Class II malocclusion dental classification and required bilateral extraction oftheir maxillary first premolars, underwent a session of professional oral hygiene and received oral hygiene instructionsbefore and during the period of study, one month later fixed orthodontic appliance were placed in the upper archby using 0.014 nickel titanium arch wire. The unstimulated whole saliva was taken from each sample immediatelybefore placement of the appliance (baseline), and at (after1hour, after one week and after two week) followingplacement of the fixed orthodontic appliance. In addition the plaque index and gingival index were recordedduring the interval periods of this study to assess oral cleanliness. The interleukin – one beta and tumor necrosis factoralphawere determined by enzyme linked immunosorbent assay, while the C-reactive protein was determined bylatex agglutination.Results: The results of the present study found the mean value of both salivary (interleukin-one beta and tumornecrosis factor -alpha) were highest at (after1hour) followed by at (after one week) then at (after two week) thanthe baseline with highly statistical significant differences (P< 0.01) among males, females and total samples, inaddition there were no statistical significant differences between males and females (P>0.05). While the mean valuesof C-reactive protein were higher at (after 1hour) only with highly significant differences (P< 0.01) among femalesand total samples while only significant difference (P<0.05) for males, in addition there were no statistical significantdifferences between males and females. Regarding the correlation between salivary (interleukin – one beta andtumor necrosis factor -alpha), there were positive correlation between them at all periods of study. Moreover therewere positive correlation between salivary (interleukin – one beta and tumor necrosis factor -alpha) and salivary Creactiveprotein. On the other hand there were no association between the salivary (interleukin – one beta andtumor necrosis factor -alpha and C-reactive protein) and clinical parameter (plaque index and gingival index).Conclusion: From this clinical study we conclude that orthodontic force induces increasing the levels of (interleukin –one beta, tumor necrosis factor -alpha, C-reactive protein) in unstimulated whole saliva during orthodontic toothmovement.


Article
Clinical Correlation and Immunological Study about Th1 Cytokines Profile in Patients with Alopecia Areata

Author: Ifad Kerim Abd Alshibly
Journal: Journal of University of Babylon مجلة جامعة بابل ISSN: 19920652 23128135 Year: 2016 Volume: 24 Issue: 7 Pages: 1916-1924
Publisher: Babylon University جامعة بابل

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Abstract

Several studies have shown that within the cascade of pathogenesis of alopecia areata, cytokines and other molecules that coordinate cyclical hair growth play a crucial role. In this research, we attempt to review the immunological role of some of Th1 cytokines namely γ-INF and TNF-α, in the pathogenesis of alopecia areata, alongside with the exploration of their clinical correlation to disease severity. This is a case controlled study conducted upon fifty four patients (twenty nine males and twenty five females, mean age 32 years) complaining from alopecia areata, and thirty apparently healthy subjects (who are age and sex-matched with the patients group). All patients and the control subjects were investigated for serum concentrations of γ-IFN, and TNF-α through a solid phase sandwich enzyme amplified sensitivity immunoassay (EASIA) . Results about the serum concentrations of γ-INF and TNF-α evaluated in this study shows that there is a significant difference in the concentrations of these two cytokines between patients of alopecia areata and control group(P<0.05). Serum concentrations of γ-INF and TNF-α were also found to be logically clinically correlated to the size of affected area of hair loss among patients with severe type of alopecia areata.

أظهرت عدة دراسات أن السيتوكينات وجزيئات أخرى تنسق نمو الشعر الدوري وتلعب دوراً حاسما داخل تتالي الحالة المرضية من الحاصة البقعية. في هذا البحث، نحاول استعراض الدور المناعي لبعض سيتوكينات Th1 هي γ-INF و TNF-α في الآلية المرضية الثعلبة، جنبا إلى جنب مع استكشاف ارتباطها السريري لشدة المرض . هذه دراسة للحالة والحالة المسيطرة أجريت على أربعة وخمسين مريضا (الذكور تسعة والعشرين و الإناث خمسة وعشرين ، متوسط عمر 32 عاماً) يشكون من الحاصة البقعية، ومجموع ثلاثين حالة سيطرة (الذين هم العمر والجنس متطابق مع مجموعة المرضى). تم التحليل المختبري في جميع المرضى و السيطرة الاصحاء لتركيزات المصل من γ-IFN و TNF-α عن طريق الاختبار المناعي (EASIA) وكانت النتائج عن تركيزات مصل γ-INF و TNF-α التي تم تقييمها في هذه الدراسة تبين أن هناك فرق كبير في تركيزات هذه السيتوكينات بين المرضى من الحاصة البقعية ومجموعة السيطرة (P<0.05). كما وجدت تركيزات مصل γ-INF و TNF-α تكون مرتبطة ارتباطاً منطقياً سريرياً بحجم المنطقة المتأثرة من تساقط الشعر بين المرضى مع النوع الشديد من الحاصة البقعية.


Article
High sensitivity C-reactive protein in preeclamptic (PE) women

Author: Sahar B. Aziz سحر عزيز
Journal: The Medical Journal of Tikrit مجلة تكريت الطبية ISSN: 16831813 Year: 2015 Volume: 20 Issue: 1 Pages: 55-62
Publisher: Tikrit University جامعة تكريت

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